A lateral flow protein microarray for rapid determination of contagious bovine pleuropneumonia status in bovine serum

Gantelius, Jesper; Hamsten, Carl; Neiman, Maja; Schwenk, Jochen M.; Persson, Anja Bondke; Andersson-Svahn, Helene

doi:10.1016/j.mimet.2010.03.007

Cited by 29 publications

(19 citation statements)

References 23 publications

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“…The benefits of LFAs include cost‐effectiveness and quick response time at POC settings . The simplicity of the lateral flow readout has been demonstrated to be favorable; however, portable, user‐friendly POC platforms require higher sensitivity, accuracy, and multiplexing capability . Multiplexing capability of microarray‐based LFAs requires accurate positioning for flow between upstream and downstream spots .…”

Section: Introductionmentioning

confidence: 99%

Lateral and Vertical Flow Assays for Point‐of‐Care Diagnostics

Jiang

Ahmed

Damayantharan

et al. 2019

Adv Healthcare Materials

138

117

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Lateral flow assays (LFAs) have been the pillar of rapid point‐of‐care (POC) diagnostics due to their simplicity, rapid process, and low cost. Recent advances in sensitivity, selectivity, and chemical stability enhancement have ensured the foothold of LFAs in commercial POC diagnostics. This paper reviews recent developments in labeling strategies and detection methods of LFAs. Moreover, vertical flow assays (VFAs) have emerged as an alternate paper‐based assay due to faster detection time and unique multiplexing capabilities. Smartphones as LFA readers have been transformed into a universal integrated platform for imaging, data processing, and storage, providing quantitative results in low‐resource settings. Commercial LFAs and VFAs products are evaluated with regards to their performance, market trends, and regulatory issues. The future outlook of the flow‐based assays for POC diagnostics is also discussed.

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Section: Introductionmentioning

confidence: 99%

Lateral and Vertical Flow Assays for Point‐of‐Care Diagnostics

Jiang

Ahmed

Damayantharan

et al. 2019

Adv Healthcare Materials

138

117

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“…The VFM concept overcomes a limitation of multiplexed lateral flow assays in which downstream binding surfaces may be affected by upstream ones (13 ); all spots are separate and independent of each other. The VFM assay format consists of a syringe filter commonly used in other applications to filter large volumes of liquids on laboratory scale.…”

Section: Design and Optimization Of The Vfm Assaymentioning

confidence: 99%

“…However, paper-based assays have typically had low sensitivity and high variability as well as a lack of multiplexing ability. Furthermore, in multiplexed lateral flow assays such as lateral flow microarrays, microspots often need to be positioned with care so that upstream spots do not alter the sample in a way that affects the detection on a downstream spot (13 ). To address these concerns, researchers have recently developed flow-through assays in which reagents are applied vertically (14 -16 ).…”

mentioning

confidence: 99%

Point-of-Care Vertical Flow Allergen Microarray Assay: Proof of Concept

et al. 2014

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BACKGROUND: Sophisticated equipment, lengthy protocols, and skilled operators are required to perform protein microarray-based affinity assays. Consequently, novel tools are needed to bring biomarkers and biomarker panels into clinical use in different settings. Here, we describe a novel paper-based vertical flow microarray (VFM) system with a multiplexing capacity of at least 1480 microspot binding sites, colorimetric readout, high sensitivity, and assay time of Ͻ10 min before imaging and data analysis.

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“…These challenges are largely avoided in immunochromatographic/lateral flow-based tests, and to this end we previously demonstrated the feasibility of combining a lateral flow/dipstick assay framework commonly used in simplex/low-plex rapid tests with protein microarray patterning for rapid and accurate determination of levels of bovine IgG raised towards mycoplasma infection [7] using a cell phone digital camera or consumer table top scanner for array imaging.…”

Section: Introductionmentioning

confidence: 99%

A Lateral Flow Protein Microarray for Rapid and Sensitive Antibody Assays

Gantelius

Bass

Sjöberg

et al. 2011

IJMS

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Protein microarrays are useful tools for highly multiplexed determination of presence or levels of clinically relevant biomarkers in human tissues and biofluids. However, such tools have thus far been restricted to laboratory environments. Here, we present a novel 384-plexed easy to use lateral flow protein microarray device capable of sensitive (<30 ng/mL) determination of antigen-specific antibodies in ten minutes of total assay time. Results were developed with gold nanobeads and could be recorded by a cell-phone camera or table top scanner. Excellent accuracy with an area under curve (AUC of 98% was achieved in comparison with an established glass microarray assay for 26 antigen-specific antibodies. We propose that the presented framework could find use in convenient and cost-efficient quality control of antibody production, as well as in providing a platform for multiplexed affinity-based assays in low-resource or mobile settings.

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A lateral flow protein microarray for rapid determination of contagious bovine pleuropneumonia status in bovine serum

Cited by 29 publications

References 23 publications

Lateral and Vertical Flow Assays for Point‐of‐Care Diagnostics

Lateral and Vertical Flow Assays for Point‐of‐Care Diagnostics

Point-of-Care Vertical Flow Allergen Microarray Assay: Proof of Concept

A Lateral Flow Protein Microarray for Rapid and Sensitive Antibody Assays

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