A Leeuwenhoek-Type Mobile Microscope for Histology Education

Maeda, Masao; Usuda, Nobuteru; Kokubo, Masahiro; Shirane, Sumito; Fukasawa, Motoaki; Nagayama, Kuniaki

doi:10.1017/s155192952000108x

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…In addition, it can support various situations such as the requirement of high-sensitivity, time-lapse shooting, stream acquisition, and long exposure. Because of these strong capabilities, several smartphone-based methods for analyzing biological phenomena have been reported [ 3 , 4 , 5 , 6 , 7 ]. Fluorescent tags are the tools to perform imaging in combination with smartphones, and various fluorescently labeled targets have been detected by a smartphone camera [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Smartphone-Based Portable Bioluminescence Imaging System Enabling Observation at Various Scales from Whole Mouse Body to Organelle

Hattori

Shirane²,

Matsuda

et al. 2020

Sensors

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Current smartphones equipped with high-sensitivity and high-resolution sensors in the camera can respond to the needs of low-light imaging, streaming acquisition, targets of various scales, etc. Therefore, a smartphone has great potential as an imaging device even in the scientific field and has already been introduced into biomolecular imaging using fluorescence tags. However, owing to the necessity of an excitation light source, fluorescence methods impair its mobility. Bioluminescence does not require illumination; therefore, imaging with a smartphone camera is compact and requires minimal devices, thus making it suitable for personal and portable imaging devices. Here, we report smartphone-based methods to observe biological targets in various scales using bioluminescence. In particular, we demonstrate, for the first time, that bioluminescence can be observed in an organelle in a single living cell using a smartphone camera by attaching a detachable objective lens. Through capturing color changes with the camera, changes in the amount of target molecules was detected using bioluminescent indicators. The combination of bioluminescence and a mobile phone makes possible a compact imaging system without an external light source and expands the potential of portable devices.

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Section: Introductionmentioning

confidence: 99%

Smartphone-Based Portable Bioluminescence Imaging System Enabling Observation at Various Scales from Whole Mouse Body to Organelle

Hattori

Shirane²,

Matsuda

et al. 2020

Sensors

Self Cite

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The extrafloral nectary traits of woody plants in Brazil’s Caatinga: describing an ecological spectrum

Cavalcante,

Câmara,

Falcão

et al. 2024

Biological Journal of the Linnean Society

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Plants use extrafloral nectaries (EFNs) as indirect defence mechanisms against herbivores. These structures contain nectar that is offered to ants in exchange for their protection. EFNs display pronounced functional variation, but it is unknown how this variation comes together in phenotypes. Here, we characterized the main functional traits of EFNs and investigated the associations among them. This work was conducted at a study site in the Caatinga that hosts 14 species of woody plants. We characterized the following functional traits: EFN type, position, arrangement, size, and reducing-sugar level. We observed a marked degree of trait variation (~30%) that was manifested in species-specific trait combinations, giving rise to an ‘ecological spectrum’. At one end were Fabaceae species with large, vascularized EFNs that occur individually on the leaf petiole and/or rachis and that produce high levels of reducing sugars. At the other end were Euphorbiaceae species with small, nonvascularized EFNs that are generally grouped on the leaf blade and that produce low levels of reducing sugars. Despite its limited geographical and phylogenetic scale, this study represents an important first step in describing an ecological spectrum that can inform our understanding of the ecological interactions and evolutionary history of this functionally relevant group of plants.

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The XyloPhone: toward democratizing access to high-quality macroscopic imaging for wood and other substrates

Wiedenhoeft

2020

IAWA J.

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One rate-limiting factor in the fight against illegal logging is the lack of powerful, affordable, scalable wood identification tools for field screening. Computer vision wood identification using smartphones fitted with customized imaging peripherals offers a potential solution, but to date, such peripherals suffer from one or more weaknesses: low image quality, lack of lighting control, uncontrolled magnification, unknown distortion, and spherical aberration, and/or no access to or publication of the system design. To address cost, optical concerns, and open access to designs and parameters, I present the XyloPhone, a 3D printed research quality macroscopic imaging attachment adaptable to virtually any smartphone. It provides a fixed focal distance, exclusion of ambient light, selection of visible light or UV illumination, uses the lens from a commercially available loupe, is powered by a rechargeable external battery, is fully open-sourced, at a price point of less than USD 110 is a highly affordable tool for the laboratory or the field, and can serve as the foundational hardware for a scalable field-deployable computer vision wood identification system.

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A Leeuwenhoek-Type Mobile Microscope for Histology Education

Abstract: Abstract:

Cited by 3 publications

References 10 publications

Smartphone-Based Portable Bioluminescence Imaging System Enabling Observation at Various Scales from Whole Mouse Body to Organelle

Smartphone-Based Portable Bioluminescence Imaging System Enabling Observation at Various Scales from Whole Mouse Body to Organelle

The extrafloral nectary traits of woody plants in Brazil’s Caatinga: describing an ecological spectrum

The XyloPhone: toward democratizing access to high-quality macroscopic imaging for wood and other substrates

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