2022
DOI: 10.1039/d2cb00030j
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A library of Rhodamine6G-based pH-sensitive fluorescent probes with versatile in vivo and in vitro applications

Abstract: Acidic pH is critical to the function of the gastrointestinal system, bone-resorbing osteoclasts, and the endolysosomal compartment of nearly every cell in the body. Non-invasive, real-time fluorescence imaging of acidic...

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Cited by 7 publications
(4 citation statements)
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“…Compared with that in an acidic environment (1 < pH < 6), the fluorescence intensity in a neutral environment will be slightly reduced. 29,30 The detection result will be a little higher than the real value without regulating pH. Considering that alcoholic beverages are generally acidic, to avoid the PL fluctuation influenced by pH, a slight adjustment to neutral pH was carried out with NaOH solution before detection.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Compared with that in an acidic environment (1 < pH < 6), the fluorescence intensity in a neutral environment will be slightly reduced. 29,30 The detection result will be a little higher than the real value without regulating pH. Considering that alcoholic beverages are generally acidic, to avoid the PL fluctuation influenced by pH, a slight adjustment to neutral pH was carried out with NaOH solution before detection.…”
Section: Resultsmentioning
confidence: 99%
“…Compared with that in an acidic environment (1 < pH < 6), the uorescence intensity in a neutral environment will be slightly reduced. 29,30 The detection result will be a little higher than the real value without regulating pH.…”
Section: Analytical Methods Papermentioning
confidence: 96%
“…These fluorescent dyes were selected because their fluorescence was shown to be independent of the pH value in a range from pH 3 to pH 10, and because both R-6G and sRB are highly water-soluble. 74,75 The fluorescent dyes were dissolved in either formic acid buffer (pH 3), water or CAPS buffer (pH 11) and mixed with microgels to enable electrostatically-driven uptake. Subsequently, excess dye was removed by allowing the microgels to sediment and replacing the supernatant several times with the respective fresh solvent.…”
Section: (C) and (D))mentioning
confidence: 99%
“…[31] This approach augments past efforts that mostly focused on solution-based bi-functional macromers for stabilizing SPNPs. [24,30,31,[41][42][43] After EHD jetting, solid CAT-SPNPs were treated with a GA solution (20 v/v% in Ultrapure water) for 30 min. To assess potential changes in nanoparticle morphology after various GA treatment times, SEM micrographs were captured and compared to nanoparticles without GA exposure (Figure S2A, Supporting Information).…”
Section: Electrohydrodynamic Jetting Produces Cat-spnpsmentioning
confidence: 99%