A Light and Electron Microscopic Study on Pulpal Nerve Fibers in the Lower Incisor of the Mouse.

Ishizuka, Hiroshi; Hiura, Akio

doi:10.1679/aohc.55.167

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…The mouse incisor is innervated by sensory and sympathetic nerves (Ishizuka and Hiura, 1992; Johansson et al, 1992; Tabata et al, 1998; Zhang et al, 1998) but is devoid of parasympathetic nerves (Olgart, 1996; Sasano et al, 1995). Nerve fibers accompanying the arteries are located within the periarterial region, which is similar to the tunica adventitia of free arteries.…”

Section: Discussionmentioning

confidence: 99%

“…Nerve fibers accompanying the arteries are located within the periarterial region, which is similar to the tunica adventitia of free arteries. These nerves are unmyelinated fibers surrounded by S100+ glial cells (Ishizuka and Hiura, 1992; Zhang et al, 1998). We show here that Shh produced by sensory neurons of the TGG is transported through the IAN and activates Gli1 expression in adjacent periarterial mesenchymal cells (Figure 7A).…”

Section: Discussionmentioning

confidence: 99%

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Secretion of Shh by a Neurovascular Bundle Niche Supports Mesenchymal Stem Cell Homeostasis in the Adult Mouse Incisor

et al. 2014

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Mesenchymal stem cells (MSCs) are typically defined by their in vitro characteristics, and as a consequence the in vivo identity of MSCs and their niches are poorly understood. To address this issue, we used lineage tracing in a mouse incisor model and identified the neurovascular bundle (NVB) as an MSC niche. We found that NVB sensory nerves secrete Shh protein, which activates Gli1 expression in periarterial cells that contribute to all mesenchymal derivatives. These periarterial cells do not express classical MSC markers used to define MSCs in vitro. In contrast, NG2+ pericytes represent an MSC subpopulation derived from Gli1+ cells; they express classical MSC markers and contribute little to homeostasis but are actively involved in injury repair. Likewise, incisor Gli1+ cells but not NG2+ cells exhibit typical MSC characteristics in vitro. Collectively, we demonstrate that MSCs originate from periarterial cells and are regulated by Shh secretion from a NVB.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Secretion of Shh by a Neurovascular Bundle Niche Supports Mesenchymal Stem Cell Homeostasis in the Adult Mouse Incisor

et al. 2014

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“…It was suggested to participate in neuronal pathfinding and branching during development and regeneration, by integrating plasma membrane and cytoskeletal responses to extracellular signals [36]. Staining for GAP-43 allows the visualization of growth cone, at the tip of axons [37,38]. More surprisingly, GAP-43 is also expressed by Schwann cells in rat molars [39].…”

Section: Innervation In the Odontoblast Layermentioning

confidence: 99%

Experimental Design for the Innervation of Tooth Forming from Implanted Cell Re-associations

Kökten¹,

Lesot²,

Kuchler‐Bopp³

2014

Cells and Biomaterials in Regenerative Medicine

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“…Although these specific structures have not been searched for in implanted cell re-associations, growth cones have been described in the developing teeth in rodents [30], [31]. Other studies have shown that CsA stimulated axonal growth and induced GAP-43 expression [32].…”

Section: Discussionmentioning

confidence: 99%

Immunomodulation Stimulates the Innervation of Engineered Tooth Organ

et al. 2014

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The sensory innervation of the dental mesenchyme is essential for tooth function and protection. Sensory innervation of the dental pulp is mediated by axons originating from the trigeminal ganglia and is strictly regulated in time. Teeth can develop from cultured re-associations between dissociated dental epithelial and mesenchymal cells from Embryonic Day 14 mouse molars, after implantation under the skin of adult ICR mice. In these conditions however, the innervation of the dental mesenchyme did not occur spontaneously. In order to go further with this question, complementary experimental approaches were designed. Cultured cell re-associations were implanted together with trigeminal ganglia for one or two weeks. Although axonal growth was regularly observed extending from the trigeminal ganglia to all around the forming teeth, the presence of axons in the dental mesenchyme was detected in less than 2.5% of samples after two weeks, demonstrating a specific impairment of their entering the dental mesenchyme. In clinical context, immunosuppressive therapy using cyclosporin A was found to accelerate the innervation of transplanted tissues. Indeed, when cultured cell re-associations and trigeminal ganglia were co-implanted in cyclosporin A-treated ICR mice, nerve fibers were detected in the dental pulp, even reaching odontoblasts after one week. However, cyclosporin A shows multiple effects, including direct ones on nerve growth. To test whether there may be a direct functional relationship between immunomodulation and innervation, cell re-associations and trigeminal ganglia were co-implanted in immunocompromised Nude mice. In these conditions as well, the innervation of the dental mesenchyme was observed already after one week of implantation, but axons reached the odontoblast layer after two weeks only. This study demonstrated that immunodepression per se does stimulate the innervation of the dental mesenchyme.

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A Light and Electron Microscopic Study on Pulpal Nerve Fibers in the Lower Incisor of the Mouse.

Cited by 7 publications

References 18 publications

Secretion of Shh by a Neurovascular Bundle Niche Supports Mesenchymal Stem Cell Homeostasis in the Adult Mouse Incisor

Secretion of Shh by a Neurovascular Bundle Niche Supports Mesenchymal Stem Cell Homeostasis in the Adult Mouse Incisor

Experimental Design for the Innervation of Tooth Forming from Implanted Cell Re-associations

Immunomodulation Stimulates the Innervation of Engineered Tooth Organ

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