A Light‐Driven DNA Nanomachine for the Efficient Photoswitching of RNA Digestion

Zhou, Mengguang; Liang, Xingguo; Mochizuki, Toshio; Asanuma, Hiroyuki

doi:10.1002/ange.200907082

Cited by 140 publications

(50 citation statements)

References 37 publications

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“…42 Importantly, this strategy offers significant advantages because it is synthetically facile and compatible with 2-photon and visible irradiation, in contrast to the recently developed azo-benzene and caged-nucleotide based approaches that require excitation at UV wavelengths. 43,44 …”

Section: Resultsmentioning

confidence: 99%

Catalytic Deoxyribozyme-Modified Nanoparticles for RNAi-Independent Gene Regulation

et al. 2012

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DNAzymes are catalytic oligonucleotides with important applications in gene regulation, DNA computing, responsive soft materials, and ultrasensitive metal-ion sensing. The most significant challenge for using DNAzymes in vivo pertains to non-toxic delivery and maintaining function inside cells. We synthesized multivalent deoxyribozyme ‘10–23’ gold nanoparticle (DzNP) conjugates, varying DNA density, linker length, enzyme orientation, and linker composition in order to study the role of the steric environment and gold surface chemistry on catalysis. DNAzyme catalytic efficiency was modulated by steric packing and proximity of the active loop to the gold surface. Importantly, the 10–23 DNAzyme was asymmetrically sensitive to the gold surface, and when anchored through the 5′ terminus was inhibited 32-fold. This property was used to generate DNAzymes whose catalytic activity is triggered by thiol displacement reactions or by photoexcitation at λ = 532 nm. Importantly, cell studies revealed that DzNPs are less susceptible to nuclease degradation, readily enter mammalian cells, and catalytically down-regulate GDF15 gene expression levels in breast cancer cells, thus addressing some of the key limitations in the adoption of DNAzymes for in vivo work.

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Section: Resultsmentioning

confidence: 99%

Catalytic Deoxyribozyme-Modified Nanoparticles for RNAi-Independent Gene Regulation

et al. 2012

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“…Photocontrol of nucleotide hybridization or binding properties can be achieved by introducing a photochromic group into site on the nucleotide [25,26,[91][92][93][94]. The photochromic species could be incorporated into the strand backbone in place of a nucleobase or ribose moiety or also by appending a photoswitch to an existing base pair.…”

Section: Photoswitchable Nucleotidesmentioning

confidence: 99%

Photochromic Materials in Biochemistry

Wilson

Branda

2016

Photochromic Materials: Preparation, Properties and Applications

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“…DNA can also be employed as DNA photoinduced enzyme for RNA 10 digestion. 68 However, the individual molecules of such devices are too small to be analysed at molecular resolution using microscopy techniques. On the other hand, DNA origami, having sizes from hundreds of nanometers up to microscale, can be visualised by atomic force microscopy or by electron microscopy.…”

Section: Biosensor Devices: From Single Molecule Sensing To Dnaamplifmentioning

confidence: 99%

Hybrid, multiplexed, functional DNA nanotechnology for bioanalysis

Wang

Arrabito

2015

Analyst

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We herein aim to report on the fabrication of DNA nano-heterostructures usable as a robust multi-functional analytical system to obtain multiple and complex data in parallel format from a single sample with unprecedented analytical performances. The ability of chemical information contained in the sequences of programmed DNA structures to organize matter made DNA become a unique material in "the nanoworld". Such carefully designed DNA nanostructures can then be functionalized/templated with different biomolecules/nanomaterials as different as nanoparticles, nanowires, organic molecules, peptides, and proteins with controlled spacing on the nanometer scale (<10 nm). In this way, it is possible to combine the properties of both DNA and nanomaterials for exposing the designed functionality and customizable geometrical hetero-nanostructures. By coupling automated on-chip high yield DNA synthesis with low cost detection methods, DNA-nanotechnology can enable the realization of high-sensitivity, multiplexed bioanalytical assays for many different applications like diagnostics, drug screening, toxicology, immunology and biosensors.

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A Light‐Driven DNA Nanomachine for the Efficient Photoswitching of RNA Digestion

Cited by 140 publications

References 37 publications

Catalytic Deoxyribozyme-Modified Nanoparticles for RNAi-Independent Gene Regulation

Catalytic Deoxyribozyme-Modified Nanoparticles for RNAi-Independent Gene Regulation

Photochromic Materials in Biochemistry

Hybrid, multiplexed, functional DNA nanotechnology for bioanalysis

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