A light scattering model for total internal reflection microscopy of geometrically anisotropic particles

Doicu, Adrian; Vasilyeva, A. A.; Efremenko, Dmitry; Wirth, Christopher L.; Wriedt, Thomas

doi:10.1080/09500340.2019.1605005

Cited by 12 publications

(10 citation statements)

References 20 publications

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“…One would expect the morphology intensity to be the main reporter of separation distance for SMR-TIRM, in particular, at a fixed orientation. 31 Herein, we now proceed to evaluate how orientation affects the scattered light intensity by the ellipsoids in an evanescent field.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In addition to the measurement of angular position, measurement of fluctuations in height is also an essential part of a TIRM experiment. One would expect the morphology intensity to be the main reporter of separation distance for SMR-TIRM, in particular, at a fixed orientation . Herein, we now proceed to evaluate how orientation affects the scattered light intensity by the ellipsoids in an evanescent field.…”

Section: Resultsmentioning

confidence: 99%

“…We systematically mapped scattered light from particles of known aspect ratio, orientation, and position. This strategy was carried out with both experiments and simulation 31 , allowing us to determine the dependence of morphology at these various conditions. We found that both the intensity and morphology of light scattered by an ellipsoid were dependent upon particle orientation in both the azimuthal and polar angles.…”

Section: Herein We Summarize the Initial Development Of Scattering Morphology Resolved Totalmentioning

confidence: 99%

“…In addition to the measurement of angular position, measurement of fluctuations in height are also an essential part of a TIRM experiment. One would expect the morphology intensity to be the main reporter of separation distance for SMR-TIRM, in particular at a fixed orientation 31 .…”

Section: 𝑎mentioning

confidence: 99%

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Developing Scattering Morphology Resolved Total Internal Reflection Microscopy (SMR-TIRM) for Orientation Detection of Colloidal Ellipsoids

et al. 2020

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Micrometer scale colloidal particles experiencing ∼kT scale interactions and suspended in a fluid are relevant to a broad spectrum of applications. Often, colloidal particles are anisotropic, either by design or by nature. Yet, there are few techniques by which ∼kT scale interactions of anisotropic particles can be measured. Herein, we present the initial development of scattering morphology resolved total internal reflection microscopy (SMR-TIRM). The hypothesis of this work is that the morphology of light scattered by an anisotropic particle from an evanescent wave is a sensitive function of particle orientation. This hypothesis was tested with experiments and simulations mapping the scattered light from colloidal ellipsoids at systemically varied orientations. Scattering morphologies were first fitted with a two-dimensional (2D) Gaussian surface. The fitted morphology was parameterized by the morphology’s orientation angle M ϕ and aspect ratio M AR. Data from both experiments and simulations show M ϕ to be a function of the particle azimuthal angle, while M AR was a sensitive function of the polar angle. This analysis shows that both azimuthal and polar angles of a colloidal ellipsoid could be resolved from scattering morphology as well or better than using bright-field microscopy. The integrated scattering intensity, which will be used for determining the separation distance, was also found to be a sensitive function of particle orientation. A procedure for interpreting these confounding effects was developed that in principle would uniquely determine the separation distance, the azimuthal angle, and the polar angle. Tracking these three quantities is necessary for calculating the potential energy landscape sampled by a colloidal ellipsoid.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Herein We Summarize the Initial Development Of Scattering Morphology Resolved Totalmentioning

confidence: 99%

Section: 𝑎mentioning

confidence: 99%

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Developing Scattering Morphology Resolved Total Internal Reflection Microscopy (SMR-TIRM) for Orientation Detection of Colloidal Ellipsoids

et al. 2020

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“…The approximate range of distribution parameters following RR distribution was

, which was inferred from the particle size range of

. The inversion error was defined as follows ( Doicu et al, 2019 ; Shcherbakov et al, 2019 ; Zhang et al, 2019 ):

…”

Section: Results and Analysismentioning

confidence: 99%

Inversion method of particle size distribution of milk fat based on improved MPGA

Ding

Zhou

et al. 2022

Front. Bioeng. Biotechnol.

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Milk fat’s particle size and distribution not only affect product quality, but also have great impacts on food safety in the economy and society. Based on total light scattering method, this paper has studied the inversion method of particle size distribution under dependent mode condition by combining multi-population genetic algorithm (MPGA) with Tikhonov smooth function. It has minimized the influence from light-absorb medium to improve the inversion accuracy. The approach introduces Tikhonov smooth function and apparent optical parameters to build an objective fitness function and weaken the ill condition of the particle size inversion equation. It also introduces multi-population genetic algorithm to solve the premature convergence of genetic algorithms. The results show that the relative error of the milk fat simulation solution with a nominal diameter is -3.52%, which meets the national standard of ±8% and better than the relative error of -5.01% of the standard genetic algorithm. Thus, the improved MPGA can reconstruct particle size distribution, with a good reliability and stability.

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Light Scattering, Absorption, Extinction, and Propagation in the Terrestrial Atmosphere

Efremenko

Kokhanovsky²

2021

Foundations of Atmospheric Remote Sensing

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A light scattering model for total internal reflection microscopy of geometrically anisotropic particles

Cited by 12 publications

References 20 publications

Developing Scattering Morphology Resolved Total Internal Reflection Microscopy (SMR-TIRM) for Orientation Detection of Colloidal Ellipsoids

Developing Scattering Morphology Resolved Total Internal Reflection Microscopy (SMR-TIRM) for Orientation Detection of Colloidal Ellipsoids

Inversion method of particle size distribution of milk fat based on improved MPGA

Light Scattering, Absorption, Extinction, and Propagation in the Terrestrial Atmosphere

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