A Linear Pentapeptide Is a Quorum-Sensing Factor Required for
            <i>mazEF</i>
            -Mediated Cell Death in
            <i>Escherichia coli</i>

Kolodkin‐Gal, Ilana; Hazan, Ronen; Gaathon, Ariel; Carmeli, Shmuel; Engelberg‐Kulka, Hanna

doi:10.1126/science.1147248

Cited by 230 publications

(274 citation statements)

References 25 publications

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“…The quorum sensing peptide designated Extracellular Death Factor (EDF), originally described by Kolodkin-Gal et al, is responsible for the mazEF and chpBIK-mediated cell death in Escherichia coli. This intestinal commensal bacterium synthesizes this EDF peptide during logarithmic growth stages when stress-situations are induced [23,24]. Our study now enlarges the role of EDF to mammalian cells.…”

Section: Epithelial To Mesenchymal (Emt)-like Transition and Relatedsupporting

confidence: 52%

Crosstalk between the microbiome and cancer cells by quorum sensing peptides

et al. 2015

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Highlights• Some quorum sensing peptides promote colon cancer cell invasion and angiogenesis.• Quorum sensing peptide Phr0662 influences tumor progression by EGFR targeting.• Cytokine profiles confirm the peptide's stimulatory effect on metastasis in vitro.• The microbiome-mammals crosstalk may explain the microbiome's effect on health. ABSTRACTTo date, the precise role of the human microbiome in health and disease states remains largely

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Section: Epithelial To Mesenchymal (Emt)-like Transition and Relatedsupporting

confidence: 52%

Crosstalk between the microbiome and cancer cells by quorum sensing peptides

et al. 2015

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“…A recent paper showed that bacterial communication, through indole signaling in stationary phase, also induces an increase in which might benefit the surrounding clonal population. 20 This is dependent on cell density via a quorum sensing-like signal (extracellular death factor/EDF 51 ). An interesting recent report demonstrated that MazF cleaves to remove the 3' end of 16S rRNA containing the anti-Shine-Dalgarno sequence, thus committing a fraction of the ribosomes to the translation of leaderless mRNAs.…”

Section: Stochastic or Deterministic Generation Of Persisters?mentioning

confidence: 99%

The toxin-antitoxin systemtisB-istR1

Wagner

Unoson

2012

RNA Biology

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“…We deduced 23 new sequences from the zwf alignment of EDF. In addition to these sequences, we synthesized three amidated forms that might be present in vivo as was described for the original EDF (Kolodkin-Gal et al 2007). Together with the known EDF peptide from E. coli NNWDN and its in vivo amidated form NNWNN, this resulted in a library of in total 29 EDF peptide variants (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Bacillus globigii cell size is influenced by variants of the quorum sensing peptide extracellular death factor

Sijbrandij

Kaman

Ligtenberg

et al. 2013

Antonie van Leeuwenhoek

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Toxin-antitoxin modules are necessary for the mode of action of several antibiotics. One of the most studied toxin-antitoxin modules is the quorum sensing-dependent MazEF system in Escherichia coli. The quorum sensing factor in this system is called the extracellular death factor (EDF), a linear pentapeptide with the sequence NNWNN. In spite of the extensive research on the mazEF system and the involvement of the quorum sensing factor EDF, the effect of EDF itself on bacteria has not yet been studied. In this research, we determined the effect of EDF and variants on cell growth in the Gram-negative bacterium E. coli and the Gram-positive Bacillus globigii. By aligning the zwf gene (from where EDF originates) of different bacterial species, we found 27 new theoretical variants of the peptide. By evaluating growth curves and light microscopy we found that three EDF variants reduced bacterial cell size in B. globigii, but not in E. coli. The D-peptides did not affect cell size, indicating that the effect is stereospecific. Peptides wherein tryptophan was substituted by alanine also did not affect cell size, which indicates that the effect seen is mediated by an intracellular target.

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A Linear Pentapeptide Is a Quorum-Sensing Factor Required for mazEF -Mediated Cell Death in Escherichia coli

Cited by 230 publications

References 25 publications

Crosstalk between the microbiome and cancer cells by quorum sensing peptides

Crosstalk between the microbiome and cancer cells by quorum sensing peptides

The toxin-antitoxin systemtisB-istR1

Bacillus globigii cell size is influenced by variants of the quorum sensing peptide extracellular death factor

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