A Lipase‐Responsive Vehicle Using Amphipathic Polymer Synthesized with the Lipase as Catalyst

Abstract

We describe an enzyme-responsive polymeric vehicle, which is of great interest in controlled drug delivery, biosensing, and other related areas. The polymer synthesized using lipase as catalyst in DMSO has a favorable molecular structure that is quickly hydrolyzed by lipase in aqueous phase, and allows a fast release of encapsulated molecules.

Uniform mPEG‐b‐PMETAC enables pH‐responsive delivery of insulin

“…To achieve these objectives, increased efforts in the fabrication of nanostructured drug delivery systems such as liposomes, 2 microspheres, 3 nanogels, 4 and polymeric micelles have been observed. 5,6 Kataoka and Harada 7 first reported that polyelectrolyte could reversibly form an assembly with proteins and DNAs through electrostatic interactions. Amphiphilic and neutral-ionic block co-polymers can also spontaneously form an assembly with counter-charged macromolecules in aqueous solution, forming a core-shell or core-corona structure.…”

“…Targeted release is also expected for a full display of the function of protein drugs. To achieve these objectives, increased efforts in the fabrication of nanostructured drug delivery systems such as liposomes, microspheres, nanogels, and polymeric micelles have been observed . Kataoka and Harada first reported that polyelectrolyte could reversibly form an assembly with proteins and DNAs through electrostatic interactions.…”

Functional protein–organic/inorganic hybrid nanomaterials

“…The substitution degree of dextran reached 23% and the enzyme nanogel had remarkable regioselectivity by discriminating between the three available secondary hydroxyl groups in the glucopyranoside unit. The product of hydrophobic group‐modified dextran could be used as a biocompatible, enzyme‐responsive material for preparing smart nanoparticles for drug delivery …”

Stabilization of activity of cellulase and hemicellulase enzymes by covering with polyacrylamide layer

“…The polymer layer around individual enzyme molecules could be a) spatial polymer (e.g. inorganic/organic hybrid polymer [12][13][14][15][16][17][18] or acrylamide-bisacrylamide random copolymer [19]); (b) dendron [20]; (c) hyperbranched polymer [21]; or (d) smart or responsive polymer [22,23].…”