A Lipidomic Analysis of Docosahexaenoic Acid (22:6, ω3) Mediated Attenuation of Western Diet Induced Nonalcoholic Steatohepatitis in Male Ldlr -/- Mice

García-Jaramillo, Manuel; Lytle, Kelli A.; Spooner, Melinda H.; Jump, Donald Β.

doi:10.3390/metabo9110252

Cited by 24 publications

(42 citation statements)

References 88 publications

(138 reference statements)

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“…In comparing the REG + Air and REG + WF groups, the WF-exposed animals demonstrated a significantly higher abundance of lyso-PI (18:0) than the REG + Air group. It has been shown that increased levels of lyso-PI (18:0) in liver tissue is an indicator of hepatic inflammation [ 28 ]. The significantly higher abundance of this lipid in the livers of REG + WF exposed rats compared to all other treatments indicated the inhalation exposure to WF likely resulted in hepatic inflammation.…”

Section: Discussionmentioning

confidence: 99%

Welding fume inhalation exposure and high-fat diet change lipid homeostasis in rat liver

et al. 2020

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It is estimated that greater than 1 million workers are exposed to welding fume (WF) by inhalation daily. The potentially toxic metals found in WF are known to cause multiple adverse pulmonary and systemic effects, including cardiovascular disease, and these metals have also been shown to translocate to the liver. This occupational exposure combined with a high fat (HF) Western diet, which has been shown to cause hyperlipidemia and non-alcoholic fatty liver disease (NAFLD), has the potential to cause significant mixed exposure metabolic changes in the liver. The goal of this study was to use matrix assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) to analyze the spatial distribution and abundance changes of lipid species in Sprague Dawley rat liver maintained on a HF diet combined with WF inhalation. The results of the MALDI-IMS analysis revealed unique hepatic lipid profiles for each treatment group. The HF diet group had significantly increased abundance of triglycerides and phosphatidylinositol lipids, as well as decreased lysophosphatidic lipids and cardiolipin. Ceramide-1-phosphate was found at higher abundance in the regular (REG) diet WF-exposed group which has been shown to regulate the eicosanoid pathway involved in pro-inflammatory response. The results of this study showed that the combined effects of WF inhalation and a HF diet significantly altered the hepatic lipidome. Additionally, pulmonary exposure to WF alone increased lipid markers of inflammation.

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Section: Discussionmentioning

confidence: 99%

Welding fume inhalation exposure and high-fat diet change lipid homeostasis in rat liver

et al. 2020

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“…The high-performance liquid chromatography (HPLC) and mass spectrometry methods used for the analysis of plasma oxylipins was based on methods previously described for the analysis of oxylipins in liver ( 24 ). The analysis was performed using a Shimadzu Prominence HPLC system (Shimadzu, Columbia, MD) coupled to an Applied Biosystems 4000 QTRAP (AB SCIEX, Framingham, MA).…”

Section: Methodsmentioning

confidence: 99%

Plasma Oxylipins: A Potential Risk Assessment Tool in Atherosclerotic Coronary Artery Disease

García-Jaramillo

Bobe

et al. 2021

Front. Cardiovasc. Med.

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Background: While oxylipins have been linked to coronary artery disease (CAD), little is known about their diagnostic and prognostic potential.Objective: We tested whether plasma concentration of specific oxylipins may discriminate among number of diseased coronary arteries and predict median 5-year outcomes in symptomatic adults.Methods: Using a combination of high-performance liquid chromatography (HPLC) and quantitative tandem mass spectrometry, we conducted a targeted analysis of 39 oxylipins in plasma samples of 23 asymptomatic adults with low CAD risk and 74 symptomatic adults (≥70% stenosis), aged 38–87 from the Greater Portland, Oregon area. Concentrations of 22 oxylipins were above the lower limit of quantification in >98% of adults and were compared, individually and in groups based on precursors and biosynthetic pathways, in symptomatic adults to number of diseased coronary arteries [(1) n = 31; (2) n = 23; (3) n = 20], and outcomes during a median 5-year follow-up (no surgery: n = 7; coronary stent placement: n = 24; coronary artery bypass graft surgery: n = 26; death: n = 7).Results: Plasma levels of six quantified oxylipins decreased with the number of diseased arteries; a panel of five oxylipins diagnosed three diseased arteries with 100% sensitivity and 70% specificity. Concentrations of five oxylipins were lower and one oxylipin was higher with survival; a panel of two oxylipins predicted survival during follow-up with 86% sensitivity and 91% specificity.Conclusions: Quantification of plasma oxylipins may assist in CAD diagnosis and prognosis in combination with standard risk assessment tools.

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“…Methyl tert-butyl ether (MTBE) extraction, which has been popular recently, is undergoing various modifications and shows very good efficiency over classical methods [ 69 ]. In the study of obesity, MTBE extraction is used to isolate lipids from liver tissue [ 77 ], skeletal muscle [ 78 ], adipose tissue [ 79 ] and plasma [ 50 , 72 ].…”

Section: Methods Of Sample Preparation For Lipidomic Studiesmentioning

confidence: 99%

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

et al. 2020

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Obesity is associated with alterations in the composition and amounts of lipids. Lipids have over 1.7 million representatives. Most lipid groups differ in composition, properties and chemical structure. These small molecules control various metabolic pathways, determine the metabolism of other compounds and are substrates for the syntheses of different derivatives. Recently, lipidomics has become an important branch of medical/clinical sciences similar to proteomics and genomics. Due to the much higher lipid accumulation in obese patients and many alterations in the compositions of various groups of lipids, the methods used for sample preparations for lipidomic studies of samples from obese subjects sometimes have to be modified. Appropriate sample preparation methods allow for the identification of a wide range of analytes by advanced analytical methods, including mass spectrometry. This is especially the case in studies with obese subjects, as the amounts of some lipids are much higher, others are present in trace amounts, and obese subjects have some specific alterations of the lipid profile. As a result, it is best to use a method previously tested on samples from obese subjects. However, most of these methods can be also used in healthy, nonobese subjects or patients with other dyslipidemias. This review is an overview of sample preparation methods for analysis as one of the major critical steps in the overall analytical procedure.

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A Lipidomic Analysis of Docosahexaenoic Acid (22:6, ω3) Mediated Attenuation of Western Diet Induced Nonalcoholic Steatohepatitis in Male Ldlr -/- Mice

Cited by 24 publications

References 88 publications

Welding fume inhalation exposure and high-fat diet change lipid homeostasis in rat liver

Welding fume inhalation exposure and high-fat diet change lipid homeostasis in rat liver

Plasma Oxylipins: A Potential Risk Assessment Tool in Atherosclerotic Coronary Artery Disease

Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity

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