A liquid chromatographic–tandem mass spectrometric method for the determination of two selective thymidylate synthase inhibitors, BGC945 and BGC638, in mouse plasma

Separation-Oriented Derivatization of Native Fluorescent Compounds through Fluorous Labeling Followed by Liquid Chromatography with Fluorous-Phase

“…Many studies on the separation mechanism in LC with a fluorocarbon stationary phase have revealed that “secondary effects”, e.g., absorption to silanol residue on the particulate, and fluorophilicity affect the retention of analytes. − Therefore, LC columns with fluorocarbon stationary phases have been used to separate highly hydrophobic compounds − and structural isomers ,,− that are difficult to separate with conventional RP columns. Furthermore, drug analogues containing some trifluoromethyl structure, a C 1 fluorous unit, have been successfully determined by LC separation using a fluorous-phase column. , Because the fluorous stationary phase can retain fluorous compounds effectively, many examinations based on fluorous interaction have been conducted to collect or remove fluorous compounds ,,− and to investigate the specific separation mechanisms. − , However, to the best of our knowledge, there are no reports on quantitative analysis of fluorous-labeled compounds using a fluorous-phase LC column.…”

“…( separate highly hydrophobic compounds [22][23][24][25] and structural isomers 16,17,[26][27][28] that are difficult to separate with conventional RP columns. Furthermore, drug analogues containing some trifluoromethyl structure, a C 1 fluorous unit, have been successfully determined by LC separation using a fluorous-phase column.…”