A liquid chromatography-atmospheric pressure photoionization tandem mass spectrometric (LC-APPI-MS/MS) method for the determination of triterpenoids in medicinal plant extracts

Abstract

An analytical method using liquid chromatography-atmospheric pressure photoionization tandem mass spectrometry with toluene as a dopant was developed for the determination of triterpenes in medicinal plant extracts. The 12 compounds determined have been shown to exhibit biological activity, such as gastroprotective, hepatoprotective, anti-inflammatory, antiviral and anti-tumor effects. The parameters of the atmospheric pressure photoionization interface were optimized to obtain the highest possible sensitivity for all of the compounds. The limits of detection and quantification ranged from 0.4 to 157.9 µg l and 1.3 to 526.4 µg l , respectively. The method was validated and applied to extracts of five medicinal plants species (Mansoa alliacea (Lam.) A.H.Gentry, Bauhinia variegata var variegata, Bauhinia variegata var alboflava, Cecropia obtuse Trécul and Cecropia palmate Willd) from the Amazonian region. The concentrations of the six triterpenes quantified in the samples ranged from 0.424 mg kg for ursolic acid to 371.96 mg kg for β-amyrin, which were quantified by using the standard addition method (n = 3). Copyright © 2016 John Wiley & Sons, Ltd.

UPLC‐ESI‐QTOF‐MS2 characterisation of Cola nitida resin fractions with inhibitory effects on NO and TNF‐α released by LPS‐activated J774 macrophage and on Trypanosoma cruzi and Leishmania amazonensis

“…Furthermore, m / z 297.2593 was lighter of 18 Da from m / z 315.2705 presumably due to the loss of H 2 O. The combination of the earlier information led to the structure of erythrodiol . A compound was also found in the LC–MS analysis of F4 with almost the same MS 2 behaviour.…”

“…A second compound with the same mass value m / z 457.3659 [C 30 H 48 O 3 + H] + was detected (tR 6.33 min) in F4 and the structure of ursolic acid was attributed. However, their fragmentation pattern proposal displayed features similar to those previously reported …”

“…Since none of these fragments allowed to univocally identify the metabolite at m / z 427.3936, two structures namely α‐ and β‐amyrin were found in the literature for this metabolite. By comparing this data with those previously reported, m / z 427.3936 was assigned as β‐amyrin. Another metabolite with the same elemental composition was detected in F4 at 7.47 min.…”

Fast and comprehensive characterization of chemical ingredients in traditional Chinese herbal medicines by extractive atmospheric pressure photoionization (EAPPI) mass spectrometry

“…34,35 APCI, another frequently used ionization technique for nonpolar compounds, was compared with APPI for the analysis of triterpenoids and greater signal intensities were obtained by APPI. 36 In addition, compared with the result for the analysis of RUCU by DART, 18 more compounds such as triterpenoids were detected by EAPPI. Briefly, EAPPI is a rapid and appropriate method for the simultaneous detection of a wide range of compounds (polar and nonpolar) in TCHMs.…”

Evaluation of Mineral Composition of Embaúba (Cecropia palmata Willd. and Cecropia obtusa Trécul) Leaves and Teas by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES)

“…Existem vários estudos relacionados à avaliação fitoquímica e farmacológica de espécies de Cecropia 12,13,14 , entretanto no que diz respeito à avaliação mineral, existem poucas pesquisas relacionadas. Dentre elas, foi avaliada a acumulação de fósforo em tecidos foliares e cascas de Cecropia palmata sendo de grande importância ecológica em paisagens amazônicas com deficiência deste metal (OLIVEIRA E MOTA, 2010; OLIVEIRA E CARVALHO, 2011).…”