A liquid chromatography method for quantifying caffeine dissolution from pharmaceutical formulations into colloidal, fat-rich media

Williams, Hywel David; Ward, Robert W.; Hardy, Ian C.W.; Melia, Colin D.

doi:10.1016/j.jchromb.2010.04.032

Cited by 11 publications

(17 citation statements)

References 35 publications

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“…30 The internal standard recovery was consistent with these results, hence caffeine-internal standard peak area ratios were used to quantify drug concentrations in the various emulsions.…”

Section: Determination Of Caffeine In Dissolution Samples By Hplcsupporting

confidence: 63%

“…Quantification of caffeine in the dissolution samples was undertaken by HPLC using the method developed by Williams et al 30 In brief, 0.4 mL sample was added to 10 :g internal standard (7-$-hydroxethyltheophylline), and the mixture diluted 1:2 with ice-cold trichloroacetic acid (12%, w/v) to precipitate the milk proteins or phase separate the Intralipid R . Samples were thoroughly mixed for approximately 30 s, centrifuged (15,000 rpm, ca.…”

Section: Determination Of Caffeine In Dissolution Samples By Hplcmentioning

confidence: 99%

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Drug release from HPMC matrices in milk and fat‐rich emulsions

Williams

Nott

Barrett

et al. 2011

Journal of Pharmaceutical Sciences

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“…30 The internal standard recovery was consistent with these results, hence caffeine-internal standard peak area ratios were used to quantify drug concentrations in the various emulsions.…”

Section: Determination Of Caffeine In Dissolution Samples By Hplcsupporting

confidence: 63%

Section: Determination Of Caffeine In Dissolution Samples By Hplcmentioning

confidence: 99%

Drug release from HPMC matrices in milk and fat‐rich emulsions

Williams

Nott

Barrett

et al. 2011

Journal of Pharmaceutical Sciences

Self Cite

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“…The HPLC method utilized in this study is a previously described method with minor alterations . Briefly, product proteins were precipitated prior to sample injection into the chromatographic system via an established protocol . This involved 100 μL of sample being placed in a tube to which 200 μL of ice‐cold 20% trichloroacetic acid solution was added.…”

Section: Methodsmentioning

confidence: 99%

Caffeine content of pre‐workout supplements commonly used by Australian consumers

Desbrow

Hall

O’Connor

et al. 2018

Drug Testing and Analysis

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The stimulant properties of caffeine are often promoted in pre-workout supplements (PWS) to assist with training, reduce the perception of fatigue, and for some brands, assist body fat loss. While manufacturers of PWS often indicate the inclusion of significant amounts of caffeine, no independent verification of the caffeine content of these products exists. The aim of this investigation was to independently assess the caffeine content of popular PWS in Australia and compare these values to nutrition information panel data. Fifteen PWS were tested for their caffeine content (both within and between batches of the same product). The caffeine content of selected PWS ranged from 91 to 387 mg·serve . Only 6 of the 15 PWS nutrition information panels included details on caffeine content. The percent of caffeine present ranged from 59% to 176% of packaging claims. All but one PWS contained a variation of caffeine within and between batches that was considered "practically" significant (ie, ≥40 mg·serve variation). Consumers are likely to be exposed to large and variable caffeine doses if ingesting PWS. Product information panels do little to improve consumer awareness of likely caffeine intakes.

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“…Several authors have reported in vitro/in vivo correlation with dissolution methods where the USP Apparatus 4 is used (ichi Jinno et al, 2008;Jantratid et al, 2009;Fang et al, 2010). Despite the advantage of low-through cell to study the dissolution performance of drugs, only information about in vitro release of ACE/CAF from nano ibers and a modi ied dissolution procedure (Illangakoon et al, 2014) as well as with pharmaceutical formulations and the USP Apparatus 1 (Williams et al, 2010) has been reported.…”

Section: Figure 1: Chemical Structure Of Acetaminophen (Left) and Caffeine (Right)mentioning

confidence: 99%

Comparative in vitro dissolution studies of fixed-dose combination formulations: analgesic tablets of acetaminophen/caffeine

López

Sánchez-Badajos

Carreto-Jiménez

et al. 2021

ijrps

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A simple and rapid UV derivative method with zero-crossing determinations was developed for estimation of acetaminophen (ACE) and caffeine (CAF) in fixed-dose combination formulations. The first-derivative of standard solutions of both drugs were used and ACE and CAF were quantified at 273.0 and 216.5 nm, respectively. The method was validated, and it was applied to dissolution studies with the USP Apparatus 2 and flow-through cell (USP Apparatus 4). Dissolution profiles comparisons (generic vs reference) were carried out with model-independent and model-dependent approaches. Mean dissolution time and dissolution efficiency were calculated and significant differences, in almost all calculated parameters, were found (p<0.05). Weibull, logistic, Gompertz, and Probit models were used to fit dissolution data and Probit was the best-fit model that describes the in vitro dissolution performance of ACE and CAF. Using t50% data, derived from this fit, dissolution profiles of ACE in USP Apparatus 2 were significant different (p<0.05). The proposed UV derivative method generates reliable information that can be compared with published results. Dissolution studies of fixed-dose combination formulations are important because quality of generic drug products depends on quality of references. It is essential to maintain a post-marketing evaluation of formulations with analgesic drugs mixed with CAF to offer the population high quality medicines.

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A liquid chromatography method for quantifying caffeine dissolution from pharmaceutical formulations into colloidal, fat-rich media

Cited by 11 publications

References 35 publications

Drug release from HPMC matrices in milk and fat‐rich emulsions

Drug release from HPMC matrices in milk and fat‐rich emulsions

Caffeine content of pre‐workout supplements commonly used by Australian consumers

Comparative in vitro dissolution studies of fixed-dose combination formulations: analgesic tablets of acetaminophen/caffeine

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