A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based assay for simultaneous quantification of aldosterone, renin activity, and angiotensin II in human plasma

Chen, Fangjun; Cheng, Ziyun; Peng, Yingfei; Huang, Chao; Liu, Dan; Wang, Beili; Pan, Baishen; Guo, Wei

doi:10.1016/j.jchromb.2021.122740

Cited by 18 publications

(21 citation statements)

References 29 publications

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“…Apart from conventional HPLC, other sophisticated HPLC-based techniques have been widely applied for the determination of pharmaceuticals, including antibiotics in various samples. The referred methods include liquid chromatography—mass spectrometry (LC-MS) [ 49 , 50 ], ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) [ 51 , 52 , 53 ], and liquid chromatography linked to tandem mass spectrometry (LC-MS/MS) [ 54 , 55 ].…”

Section: Methods Used To Analyze Antibioticsmentioning

confidence: 99%

A Review of Methods for Removal of Ceftriaxone from Wastewater

Karungamye

Rugaika

Mtei

et al. 2022

JoX

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The presence of pharmaceuticals in surface water and wastewater poses a threat to public health and has significant effects on the ecosystem. Since most wastewater treatment plants are ineffective at removing molecules efficiently, some pharmaceuticals enter aquatic ecosystems, thus creating issues such as antibiotic resistance and toxicity. This review summarizes the methods used for the removal of ceftriaxone antibiotics from aquatic environments. Ceftriaxone is one of the most commonly prescribed antibiotics in many countries, including Tanzania. Ceftriaxone has been reported to be less or not degraded in traditional wastewater treatment of domestic sewage. This has piqued the interest of researchers in the monitoring and removal of ceftriaxone from wastewater. Its removal from aqueous systems has been studied using a variety of methods which include physical, biological, and chemical processes. As a result, information about ceftriaxone has been gathered from many sources with the searched themes being ceftriaxone in wastewater, ceftriaxone analysis, and ceftriaxone removal or degradation. The methods studied have been highlighted and the opportunities for future research have been described.

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Section: Methods Used To Analyze Antibioticsmentioning

confidence: 99%

A Review of Methods for Removal of Ceftriaxone from Wastewater

Karungamye

Rugaika

Mtei

et al. 2022

JoX

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“…Until the newly LC–MS/MS assays were continuously developed for PRA determination, they followed the main inhibitor regimen of RIA, in which EDTA, PMSF, SBTI, and neomycin were retained for the protection of Ang1 17 . Recently, the incubation time was cut to 3 h, and neomycin (for inhibition of bacterial peptidase during longer incubation 85 ) was less used in routine laboratories 64 . Therefore, the combination of EDTA, PMSF, and SBTI was chosen as the recommended inhibitors for LC–MS/MS analysis.…”

Section: Factors Affecting the Pra Measurement Processmentioning

confidence: 99%

“…First, for high PRA samples, sufficient incubation could reduce the proportion of blanks and make the blanks negligible. Second, some studies demonstrated that the blanks are usually below the LLOQ, and blank measurement causes sample consumption and labor costs because larger sample volumes are required for two analyses 50,52,62,64 . Samples with endogenous Ang1 above the LLOQ accounted for only 5.6%–8.5% of the generated Ang1 60,64 .…”

Section: Factors Affecting the Pra Measurement Processmentioning

confidence: 99%

“…Second, some studies demonstrated that the blanks are usually below the LLOQ, and blank measurement causes sample consumption and labor costs because larger sample volumes are required for two analyses 50,52,62,64 . Samples with endogenous Ang1 above the LLOQ accounted for only 5.6%–8.5% of the generated Ang1 60,64 . Moreover, Morganti A et al 14 found that blank subtraction led to poor reproducibility in RIA assays, especially for low renin samples 14 .…”

Section: Factors Affecting the Pra Measurement Processmentioning

confidence: 99%

“…Until now, there has been insufficient research to provide intra‐ and interindividual biological variation data or unified analytical performance specification for PRA, and a significant discrepancy in evaluation criteria was observed in the reporting and interpretation of results, such as different renin suppression thresholds (0.5 or 1 ng/ml/h) adopted in different studies. Therefore, it is necessary to establish PRA‐specific biological variation, cut‐off values, or reference intervals based on the LC–MS/MS method for healthy and hypertensive groups 64 …”

Section: Factors Affecting the Pra Measurement Processmentioning

confidence: 99%

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The spectrum of plasma renin activity and hypertension diseases: Utility, outlook, and suggestions

Liu

Jin

Zhou

et al. 2022

Clinical Laboratory Analysis

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Hypertension is a highly prevalent cardiovascular disease, which is associated with various morbidities and even mortality. [1][2][3][4] Although medications and treatment strategies are widely available, over 50% of hypertensive patients are unable to control and normal blood pressure could be hard to maintain. 5 Consequently, hypertensive adverse complications might greatly influence the quality of life and survival benefits. Nearly 90% of hypertensive patients are primary hypertension, whereas secondary hypertension makes up an increasing

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A biomarker panel of secondary hypertension is simultaneously quantified by coupling of magnetic solid‐phase extraction and liquid chromatography–tandem mass spectrometry

Gao,

Li,

Chen

et al. 2024

Rapid Comm Mass Spectrometry

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RationaleSecondary hypertension is often caused by activation of complex multi‐organ endocrine systems, while renin activity indicated by angiotensins (Angs), aldosterone (ALD) and cortisol (COR) in such systems are generally accepted as its diagnostic markers. As antibody‐based methods cannot offer comparable quantification for these biomarkers, a liquid chromatography (LC)–tandem mass spectrometry (MS/MS)‐based approach was developed to quantify them simultaneously and accurately.MethodsFive different beads for magnetic solid‐phase extraction (MSPE) were evaluated towards their enrichment efficiency for these biomarkers. An LC system with optimized elution gradient and a triple‐quadrupole MS with tuned parameters were coupled to quantitatively monitor the extracted analytes. The method performance was further examined such as linearity, precision, stability, recovery rate and matrix effect. Based on the developed method, the abundance of Ang II, ALD and COR in plasma was measured and the quantification was compared with that derived from commercial ELISA kits.ResultsAs compared with other MSPEs, Angs, ALD and COR were highly enriched by the HLB magnetic beads with satisfactory recoveries. These analytes were simultaneously quantified by LC/MS/MS and all the method parameters for quantification were well matched with the requirements of clinical testing. Comparison of the quantitative results derived from ELISA and LC/MS/MS exhibited that the two methods offered basically comparable values with Pearson r values at 0.896, 0.895 and 0.835, respectively. The stability test for plasma Angs at room temperature indicated that the abundance of Ang II was relatively stable within 3 h, whereas that of Ang I and Ang 1–7 was time‐dependently changed.ConclusionsCoupling of HLB beads and LC/MS/MS thus enables simultaneous quantification of a set of biomarkers related to secondary hypertension.

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A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based assay for simultaneous quantification of aldosterone, renin activity, and angiotensin II in human plasma

Cited by 18 publications

References 29 publications

A Review of Methods for Removal of Ceftriaxone from Wastewater

A Review of Methods for Removal of Ceftriaxone from Wastewater

The spectrum of plasma renin activity and hypertension diseases: Utility, outlook, and suggestions

A biomarker panel of secondary hypertension is simultaneously quantified by coupling of magnetic solid‐phase extraction and liquid chromatography–tandem mass spectrometry

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