A liquid crystals-based sensing platform for detection of α-amylase coupled with destruction of host-guest interaction

Ma, Hui; Kang, Qi; Wang, Tao; Yu, Li

doi:10.1016/j.colsurfb.2018.10.036

Cited by 17 publications

(11 citation statements)

References 53 publications

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“…This also suggests that the response time to detect Pb 2+ ions primarily depends on the kinetic behavior of the CTAB molecules at the LC–aqueous interface. These types of competitive binding interaction based LC sensors have been reported earlier. − Quantification of the light intensity transmitted through the film of LC, as shown in Figure a, reveals that the response time differs as a function of the concentration of Pb 2+ ions. We observed that, with the decrease in Pb 2+ concentration, the macroscopic appearance of the LC changes continuously.…”

Section: Resultssupporting

confidence: 68%

“…While the aptamer-based sensors for detection of heavy metals are being explored in other fields, this is the first report at the LC–aqueous interface, to the best of our knowledge, using aptamers that can effectively tune the orientational transitions of LC in the presence of nanomolar concentrations of the metal ion target. It is further noted that, although RNA quadruplexes are thermodynamically more stable structures than their DNA counterpart, the cost of the sensor could be made potentially cheaper if a DNA version of the aptamer is selected from a pool of DNA that have a Pb 2+ binding G-rich sequence. ,, This aptamer-based LC approach can be potentially extended as a detection tool for other toxic metal ions, and the development of such sensors is currently ongoing in our laboratory.…”

Section: Discussionmentioning

confidence: 99%

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Liquid Crystal based Detection of Pb(II) Ions Using Spinach RNA as Recognition Probe

et al. 2019

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We report a new method for label-free, sensitive, and facile detection of lead(II) ions (Pb 2+ ) based on an aptamer−target binding event, which is recognized by orientations of liquid crystals (LCs) at aqueous interfaces. The LC film suspended in the aqueous phase demonstrated a homeotropic orientation in contact with a cationic surfactant cetyltrimethylammonium bromide (CTAB) due to selfassembly of CTAB molecules at the aqueous−LC interface. The ordering of LC subsequently changed to planar in the presence of the spinach RNA aptamer (SRNA) due to interactions between CTAB and SRNA. In the presence of the Pb 2+ ion, the ordering of LC changed to homeotropic caused by reorganization of CTAB at the LC−aqueous interface. This is due to formation of more stable quadruplex structures of SRNA with Pb 2+ ions in comparison to the CTAB-SRNA complex. The sensor exhibited a detection limit of 3 nM, which is well below the permissible limit of Pb 2+ in drinking water. Our experiments establish that addition of Pb 2+ leads to (i) the formation of Pb 2+ -SRNA complexes and (ii) a decrease in density of SRNA on the LC interface, but additional studies are required to determine which of these processes underlie the response of the LCs to the Pb 2+ . We have also demonstrated the potential application of the LC sensor for detection of Pb 2+ in tap water. Unlike current laboratory-based heavy-metal-ion assays, this method is comparatively simple in terms of instrumentation, operation, and optical readout.

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Section: Resultssupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Liquid Crystal based Detection of Pb(II) Ions Using Spinach RNA as Recognition Probe

et al. 2019

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“…LC-based sensing can be designed to possess sensor properties in different elds, 20 such as biosensing, chemical-sensing, optical-sensing, gas-sensing and many others. [21][22][23][24] The design of LC-compounds based sensors for chemical and biological species based on changes in the bulk properties of LCs. For example, cholesteric LC phases have been used to detect ethanol, 22 water vapor, 25 and vaporous analytes 26 such as amines.…”

Section: Introductionmentioning

confidence: 99%

“…For example, cholesteric LC phases have been used to detect ethanol, 22 water vapor, 25 and vaporous analytes 26 such as amines. 21,23 ILCs are widespread in gas and liquid phase sensing. Thus, the interface of ILCs charges as a consequence of ion adsorption/dissociation, leading to the formation of electrical double layers at the interface of LC, which influences the ordering of LC.…”

Section: Introductionmentioning

confidence: 99%

Liquid crystal behavior, photoluminescence and gas sensing: A new series of ionic liquid crystal imidazole and benzoimidazole bearing chalcone groups, synthesis and characterization

Mohammad

Abbas

2021

RSC Adv.

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“…One group of methods measures the consumption of natural starch hydrolyzed by α-amylase. Some examples of this approach include viscometries by capillary viscometer [7], magnetoelastic cantilever [8] or piezoelectric transducer [9,10], electrochemically amperometry [11,12,13,14,15,16], potentiometry [17], colorimetry of starch-iodine [18,19] or a chromogenic substrate [20] color intensity, and liquid crystals-based sensing platform [21]. These methods generally have poor reproducibility, with large differences associated with distinct operators or slight protocol variations, because the attack speed of α-amylase is deeply influenced by varying molecular mass and branching degrees of heterogeneous starch macromolecules [22].…”

Section: Introductionmentioning

confidence: 99%

Hand-held Colorimetry Sensor Platform for Determining Salivary α-Amylase Activity and Its Applications for Stress Assessment

Hsiao

Chen

Chou

et al. 2019

Sensors

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This study develops a hand-held stress assessment meter with a chemically colorimetric strip for determining salivary α-amylase activity, using a 3,5 dinitrosalicylic acid (DNS) assay to quantify the reducing sugar released from soluble starch via α-amylase hydrolysis. The colorimetric reaction is produced by heating the strip with a mini polyester heater plate at boiling temperature to form a brick red colored product, which measured at 525 nm wavelength. This investigation describes in detail the design, construction, and performance evaluation of a hand-held α-amylase activity colorimeter with a light emitted diode (LED) and photo-detector with built-in filters. The dimensions and mass of the proposed prototype are only 120 × 60 × 60 mm3 and 200 g, respectively. This prototype has an excellent correlation coefficient (>0.995), comparable with a commercial ultraviolet–visible spectroscope, and has a measurable α-amylase activity range of 0.1–1.0 U mL−1. The hand-held device can measure the salivary α-amylase activity with only 5 μL of saliva within 12 min of testing. This sensor platform effectively demonstrates that the level of salivary α-amylase activity increases more significantly than serum cortisol, the other physiological stressor biomarker, under physiologically stressful exercise conditions. Thus, this work demonstrates that the hand-held α-amylase activity meter is an easy to use and cost-effective stress assessment tool for psychoneuroendocrinology research.

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A liquid crystals-based sensing platform for detection of α-amylase coupled with destruction of host-guest interaction

Cited by 17 publications

References 53 publications

Liquid Crystal based Detection of Pb(II) Ions Using Spinach RNA as Recognition Probe

Liquid Crystal based Detection of Pb(II) Ions Using Spinach RNA as Recognition Probe

Liquid crystal behavior, photoluminescence and gas sensing: A new series of ionic liquid crystal imidazole and benzoimidazole bearing chalcone groups, synthesis and characterization

Hand-held Colorimetry Sensor Platform for Determining Salivary α-Amylase Activity and Its Applications for Stress Assessment

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