A liquid culture system for shoot proliferation and analysis of pharmaceutically active constituents of Catharanthus roseus (L.) G. Don

Pati, Pratap Kumar; Kaur, Jaspreet; Singh, Pawandeep

doi:10.1007/s11240-010-9868-4

Cited by 60 publications

(45 citation statements)

References 35 publications

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“…On lesser volume of medium, the growth of cultures was slow and the medium dried up within a week; a volume of medium more than mentioned above caused hyperhydration of shoots, which affected shoot growth and multiplication. Reduction in the number and growth of shoots was due to hyperhydration which is a result of lower oxygen availability and submergence of shoots (Pati et al 2011). Time period of subculturing was found to be an important factor for the maintenance and multiplication of cultures.…”

Section: Shoot Multiplication In Liquid Mediummentioning

confidence: 99%

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

Vibha

Shekhawat

Mehandru

et al. 2013

Physiol Mol Biol Plants

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An efficient and improved method for in vitro propagation of mature tree of Dalbergia sissoo, an ecologically and commercially important timber yielding species, has been developed through axillary shoot proliferation. Bud breaking occurred from nodal shoot segments derived from rejuvenated shoots produced during early spring from a 20-25-year-old lopped tree, on MS medium containing 8.88 μM benzylaminopurine (BAP). Multiple shoots differentiated (20-21shoots/node) on re-culture of explants on halfstrength agar gelled amended MS medium with a combination of 2.22 μM of BAP and 0.002 μM of thidiazuron (TDZ) with 1.0 mM each of Ca(NO 3 ) 2 , K 2 SO 4 , KCl, and NH 4 (SO 4 ) 2 . The maximum shoot multiplication (29-30 shoots/node) was achieved on subculturing in the above mentioned but liquid medium. Furthermore, the problem of shoot tip necrosis and defoliation observed on solid medium were overcome by the use of liquid medium. Ex vitro rooting was achieved on soilrite after basal treatment of microshoots with 984 μM of indole-3-butyric acid (IBA) for 2 min. About 90 % microshoots were rooted on soilrite within 2-3 weeks under the greenhouse conditions. From 20 nodal shoot segments, about 435 hardened plants were acclimatized and transplanted. This is the first report for rapid in vitro propagation of mature trees of D. sissoo on liquid medium followed by ex vitro rooting.

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Section: Shoot Multiplication In Liquid Mediummentioning

confidence: 99%

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

Vibha

Shekhawat

Mehandru

et al. 2013

Physiol Mol Biol Plants

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“…Several authors have reported the value of liquid media for shoot multiplication (Russowski et al 2006;Pati et al 2011;Cavallaro et al 2014). However, S. alpina shoot tip explants placed in liquid culture, either agitated or stationary, did not derive shoots, and tissue necrosis was observed, probably caused by the continuous contact between the plant tissue and the liquid medium.…”

Section: Shoot Multiplicationmentioning

confidence: 99%

“…The elimination of agar from the culture medium also resulted in substantial savings in cost. Pati et al (2011) calculated a 5.2-fold reduction in cost associated with the use of liquid medium for Catharanthus roseus shoot multiplication.…”

Section: Quantification Of Flavones and Verbascoside In Multiple Shootsmentioning

confidence: 99%

The influence of liquid systems for shoot multiplication, secondary metabolite production and plant regeneration of Scutellaria alpina

Grzegorczyk-Karolak

Rytczak

Bielecki

et al. 2016

Plant Cell Tiss Organ Cult

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“…Over recent decades, several attempts have been made to improve withanolide production by tissue culture (Ray and Jha 2001;Sharada et al 2007;Dewir et al 2010). Production of bioactive molecules by in-vitro liquid culturing techniques has gained considerable attention in recent years, and such methods are increasingly attractive alternatives to whole plant cultivation for production of high value phytochemicals (Pati et al 2010;Gawde and Paratkar 2012). …”

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confidence: 99%

Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal

Mir

Khazir

Hakeem

et al. 2014

J. Plant Biochem. Biotechnol.

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Withania somnifera (L) Dunal, commonly known as ashwagandha or Indian ginseng, is the source of large number of pharmacologically active withanolides. Withaferin-A (WS-3), a major withanolide of W. somnifera, has been proven to be an effective anti-cancer molecule. In this study, a liquid culture system for shoot proliferation, biomass accumulation and withaferin-A production of an elite accession (AGB002) of W. somnifera was investigated. The nodal explants cultured on Murashige and Skoog (MS) semi-solid medium supplemented with various concentrations of 6-benzyl adenine (BA) and Kinetin (Kn) 2 elicited varied responses. The highest number of regenerated shoots per ex-plant (35±3.25) and the maximum average shoot length (5.0± 0.25 cm) were recorded on MS medium supplemented with BA (5.0 μM). The shoots were further proliferated in half and full strength MS liquid medium supplemented with the same concentration BA. It was interesting to note that shoots cultured on MS half strength liquid medium fortified with 4 gL-1 FW (fresh weight) shoot inoculum mass derived from 5 week old nodal explants of W. somnifera showed highest accumulation of biomass and withaferin A content in 5 weeks.Withaferin A was produced in relatively high amounts The production of Withania drugs in India has been esti-mated about 9,127-tonnes per year far exceeding the annual plant production of 5,905-tonnes (Sivanandhan et al. 2012). Presently, whole plants are being harvested from wild for the production of Withania-based medicines to meet a growing demand of pharmaceutical industries. This random harvesting of ashwagandha germplasm for withanolide production is economically and environmentally unwise as it causes loss of genetic diversity and habitat destruction. An elegant alter-native to these apparent hurdles would be to identify the genotype rich in bioactive withanolides, develop high yielding in vitro production methods and to and improve them genetically for cultivation to meet the demand of bioactive withanolides.Plant cell and organ cultures offer an excellent opportunity for homogenous, controlled production of metabolites, throughout the year, especially when we take commercial demand into account. They not only facilitate the de novo synthesis of novel compounds, but also are able to produce metabolites, even in higher amounts than in the intact plants. Over recent decades, several attempts have been made to improve withanolide production by tissue culture (Ray and Jha 2001;Sharada et al. 2007;Dewir et al. 2010). Production of bioactive molecules by in-vitro liquid culturing techniques has gained considerable attention in recent years, and such methods are increasingly attractive alternatives to whole plant cultivation for production of high value phytochemicals (Pati et al. 2010;Gawde and Paratkar 2012). 4The pure compound of WS-3 is commercially obtained from the aerial parts of W. somnifera plants but the production is not sufficient for the current market demand. The chemical synthesis of withaferin A is comp...

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A liquid culture system for shoot proliferation and analysis of pharmaceutically active constituents of Catharanthus roseus (L.) G. Don

Cited by 60 publications

References 35 publications

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

The influence of liquid systems for shoot multiplication, secondary metabolite production and plant regeneration of Scutellaria alpina

Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal

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