A ‘litmus test’ for molecular recognition using artificial membranes

Charych, Deborah H.; Cheng, Quan; Reichert, A.; Kuziemko, Geoffrey M.; Stroh, Mark; Nagy, Jon O.; Spevak, Wayne; Stevens, Raymond C.

doi:10.1016/s1074-5521(96)90287-2

Cited by 240 publications

(159 citation statements)

References 22 publications

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“…This unique chromatic property has also made polydiacetylenes promising candidates for colorimetric biosensors. 2,3,21 The architectures of amphiphilic PDA-based colorimetric biosensors reported to date were either vesicles in aqueous solutions 2,13 or thin films on solid supports generated using LangmuirBlodegtt or Langmuir-Schaefer methods, and incorporated ligands for detection of receptor binding (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

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Functional Self-Assembling Bolaamphiphilic Polydiacetylenes as Colorimetric Sensor Scaffolds

2004

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ABSTRACT. Conjugated polymers capable of responding to external stimuli by changes in optical, electrical or electrochemical properties can be used for the construction of direct sensing devices.Polydiacetylene-based systems are attractive for sensing applications due to their colorimetric response to changes in the local environment. Here we present the design, preparation and characterization of self-assembling functional bolaamphiphilic polydiacetylenes (BPDAs) inspired by Nature's strategy for membrane stabilization. We show that by placing polar headgroups on both ends of the diacetylene lipids in a transmembranic fashion, and altering the chemical nature of the polar surface residues, the conjugated polymers can be engineered to display a range of radiation-, thermaland pH-induced colorimetric responses. We observed dramatic nanoscopic morphological 2 transformations accompanying charge-induced chromatic transitions, suggesting that both side chain disordering and main chain rearrangement play important roles in altering the effective conjugation lengths of the poly(ene-yne). These results establish the foundation for further development of BPDAbased colorimetric sensors. Introduction:

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Section: Introductionmentioning

confidence: 99%

“…1). 2,21,22 PDA sensors based on ribbon morphologies have not yet been explored. These sensors integrated molecular recognition and signal transduction into one supramolecular assembly, and responded to binding events by a straightforward color change.…”

Section: Introductionmentioning

confidence: 99%

Functional Self-Assembling Bolaamphiphilic Polydiacetylenes as Colorimetric Sensor Scaffolds

2004

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“…The diacetylene monomers can be easily topochemically polymerized inside ordered lamella phase of appropriate diacetylene vesicles by photo polymerization [4]. During the polymerization, adjacent diacetylene units, transfer into a polyconjugated polydiacetylenic polymer backbone with remarkable optical properties.…”

Section: Introduction:-mentioning

confidence: 99%

“…During the polymerization, adjacent diacetylene units, transfer into a polyconjugated polydiacetylenic polymer backbone with remarkable optical properties. Different factors like temperature [2], pH [4], organic solvent [1] and mechanical stress [12] trigger Colour transition from blue to red due to conformational changes in the conjugated backbone in Polydiacetylene assemblies.The best examples of Polydiacetylene biosensors are carbohydrate functionalized PDA for the detection of the influenza virus, cholera toxin [5,21,27], and E. coli. The topochemically polymerization is achieved by self assembly of the diacetylene entities in ordered form depending on their molecular architecture and nature.…”

Section: Introduction:-mentioning

confidence: 99%

Stable Drug Delivery Systems: Peg-Polydiacetylene Liposomes.

Swapna¹

2017

IJAR

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“…PDA vesicular aggregates and films have been previously shown to undergo distinct blue-red colorimetric changes due to conformational transitions in the conjugated (ene-yne) polymer backbone, induced by external structural perturbations (9,30,35). The colorimetric transitions of PDA go hand in hand with unique fluorescence properties: no fluorescence is emitted by the initially polymerized blue-phase PDA, while the red-phase PDA strongly fluoresces at 560 nm and at 640 nm (24).…”

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confidence: 99%

Vaccinia Virus Interactions with the Cell Membrane Studied by New Chromatic Vesicle and Cell Sensor Assays

Orynbayeva¹,

Kolusheva²,

Groysman³

et al. 2007

J Virol

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The potential danger of cross-species viral infection points to the significance of understanding the contributions of nonspecific membrane interactions with the viral envelope compared to receptor-mediated uptake as a factor in virus internalization and infection. We present a detailed investigation of the interactions of vaccinia virus particles with lipid bilayers and with epithelial cell membranes using newly developed chromatic biomimetic membrane assays. This analytical platform comprises vesicular particles containing lipids interspersed within reporter polymer units that emit intense fluorescence following viral interactions with the lipid domains. The chromatic vesicles were employed as membrane models in cell-free solutions and were also incorporated into the membranes of epithelial cells, thereby functioning as localized membrane sensors on the cell surface. These experiments provide important insight into membrane interactions with and fusion of virions and the kinetic profiles of these processes. In particular, the data emphasize the significance of cholesterol/sphingomyelin domains (lipid rafts) as a crucial factor promoting bilayer insertion of the viral particles. Our analysis of virus interactions with polymer-labeled living cells exposed the significant role of the epidermal growth factor receptor in vaccinia virus infectivity; however, the data also demonstrated the existence of additional non-receptor-mediated mechanisms contributing to attachment of the virus to the cell surface and its internalization.The essential initial step in the viral infection process involves transport of the virus or its genetic components through the host cell membrane. Membrane interactions and penetration of viral particles are generally complex multistep processes determined by varied factors and molecular events that have been elucidated for only a few viral species. Indeed, revealing the mechanisms of membrane binding by viral particles, bilayer fusion with the viral coat, and eventual virus internalization into the host cell is critical for understanding viral infection and propagation. In particular, determining the contributions of virus-lipid interactions to cell entry (rather than receptormediated uptake) is essential for gaining insight into the factors affecting cross-species infectivity.Vaccinia virus (VV) is a member of the Poxviridae family, which coinfect a wide variety of mammalian cells. The two infectious forms of vaccinia virions include the intracellular mature virus (IMV) and the extracellular enveloped virus (EEV), which are structurally distinct and exhibit different biological features (1). The IMV is a single-enveloped particle containing different viral proteins on its membrane (18) and represents the majority of infectious progeny produced in cells and responsible for virus proliferation among neighboring cells. EEV has an additional outer membrane with specific proteins and corresponds to infecting distant cells (39). The precise mechanisms of VV entry into host cells have not been ful...

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A ‘litmus test’ for molecular recognition using artificial membranes

Cited by 240 publications

References 22 publications

Functional Self-Assembling Bolaamphiphilic Polydiacetylenes as Colorimetric Sensor Scaffolds

Functional Self-Assembling Bolaamphiphilic Polydiacetylenes as Colorimetric Sensor Scaffolds

Stable Drug Delivery Systems: Peg-Polydiacetylene Liposomes.

Vaccinia Virus Interactions with the Cell Membrane Studied by New Chromatic Vesicle and Cell Sensor Assays

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