A Litopenaeus vannamei TRIM32 gene is involved in oxidative stress response and innate immunity

Wang, Lei; Lu, Ke-Cheng; Chen, Guoliang; Li, Ming; Chen, Yihong

doi:10.1016/j.fsi.2020.11.002

Cited by 12 publications

(6 citation statements)

References 29 publications

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“…The cytoskeleton has four main components including actin, microtubules, intermediate filaments and septins, which all have important functions in cell-autonomous immunity [13]. An E3 ubiquitin-protein ligase TRIM32 gene was crucial under oxidase stress and during Vibrio infection in L. vannamei [14]. In intestine, the MAM and LDL-receptor class A domain-containing protein could regulate enterohepatic bile acid signaling, while bile acids are vital factors in mucosal immunity and inflammation [15,16].…”

Section: Discussionmentioning

confidence: 99%

Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection

Li¹,

Zhang²,

Du³

et al. 2023

Preprint

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Vibrio parahaemolyticus carrying a plasmid encoding toxins PirA and PirB is one of the causative agents leading to the severe disease of acute hepatopancreatic necrosis disease (AHPND) in shrimp aquaculture. However, there is a lack of deep understanding of the host resistant characteristics against V. parahaemolyticus infection. Here, we performed comparative transcriptome analysis on the expression profiles at the background level of hemocytes from shrimp with different resistant abilities against V. parahaemolyticus infection in two independent populations. Principal component analysis and sample clustering results showed that samples from the same population had a closer relationship than that from shrimp with similar disease-resistant abilities. Differentially expressed genes (DEGs) analysis revealed that the number of DEGs between two populations was much more than that between V. parahaemolyticus¬-resistant and susceptible shrimp. A total of 31 DEGs and 5 DEGs were identified from the comparison between V. parahaemolyticus¬-resistant and susceptible shrimp from population 1 and 2, respectively. There was no DEGs overlap between two comparisons. DEGs from population 1 were mainly cytoskeleton related genes, metabolic related genes, and immune related genes. DEGs from population 2 included tubulin alpha-3, dihydropyrimidinase-like gene, diacylglycerol kinase 1, prohibitin and iroquois-class homeodomain protein IRX-2-like. The data suggest that shrimp from different populations might adopt distinct disease-resistant mechanisms, which provided new insights into understanding disease-resistant mechanisms at population level against pathogen infection. A new strategy for screening disease resistant genes and disease resistant breeding was proposed based on the present study.

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Section: Discussionmentioning

confidence: 99%

Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection

Li¹,

Zhang²,

Du³

et al. 2023

Preprint

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“…Diet on Humoral Immunity of L. vannamei. Humoral immunity contains the proPO system, lectins, crustin, and lysozyme, which can trigger various immune responses through intercellular communication and plays an essential role in immune defense [61]. The proPO system is controlled and regulated by proteins capable of binding to proteinases, proteinase inhibitors, and microbial compounds, including proPO and prophenoloxidaseactivating enzyme (PPA) [26,62].…”

Section: Effects Of Macroalgaementioning

confidence: 99%

Macroalgae Improve the Growth and Physiological Health of White Shrimp (Litopenaeus vannamei)

Zhang

Shi

et al. 2023

Aquaculture Nutrition

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This study compared and evaluated the effects of nine native macroalgae species on the tropic coast of China on the growth and physiological health performance of white shrimp (Litopenaeus vannamei). Nine hundred juvenile shrimps weighing 1.6 ± 0.02 g were fed with nine different types of macroalgae for 28 days. The experimental groups were as follows: Con (the diet without macroalgae), CRA (Caulerpa racemosa), CLA (Caulerpa lentillifera), CSS (Caulerpa sertularioides), CLM (Chaetomorpha linum), ULA (Ulva lactuca), GBE (Gracilaria bailiniae), ASA (Acanthophora spicifera), SVC (Sargassum ilicifolium var. conduplicatum), and BGE (Betaphycus gelatinae). Results showed that the growth performance of shrimps fed on the macroalgae meals was significantly higher than that of the control group ( P < 0.05 ). The immune defense capacity (total hemocyte count, phagocytosis respiratory bursts, prophenoloxidase system, hemagglutination activity, and antibacterial and bacteriolytic activities) and antioxidant capacity (total antioxidant capacity, superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde) of L. vannamei fed on macroalgae meals were significantly higher than those of the control group ( P < 0.05 ). Specifically, the shrimps in the ASA group had the significantly higher physiological health level than shrimps in the other macroalgae groups ( P < 0.05 ), and the expression of immune and antioxidation-related genes was also significantly higher in the ASA group ( P < 0.05 ). Principal component analysis (PCA) demonstrated that optimal growth and physiological health efficacy were observed in the ASA group. In summary, this study suggested dietary manipulation using macroalgae to improve the growth performance, immune performance, and antioxidant capacity of L. vannamei, with the optimal macroalgae for the diet being Acanthophora spicifera.

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“…TRIM protects hosts from viral infection in a variety of ways. TRIM32 is involved in pathogen-infection resistance and the oxidative stress response in Litopenaeus vannamei [ 14 ]. TRIM5 can directly bind to virus proteins and then destroy the virus core and inhibits the replication of viruses by activating the NFκB signaling pathway [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

PcTrim prevents early infection with white spot syndrome virus by inhibiting AP1-induced endocytosis

Cao

et al. 2023

Cell Commun Signal

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Viruses have evolved various strategies to achieve early infection by initiating transcription of their own early genes via host transcription factors, such as NF-κb, STAT, and AP1. How the host copes with this immune escape has been a topic of interest. Tripartite motif (TRIM) family proteins with RING-type domains have E3 ubiquitin ligase activity and are known as host restriction factors. Trim has been reported to be associated with phagocytosis and is also believed to be involved in the activation of autophagy. Preventing the virus from entering the host cell may be the most economical way for the host to resist virus infection. The role of TRIM in the early stage of virus infection in host cells remains to be further interpreted. In the current study, a crayfish TRIM with a RING-type domain, designated as PcTrim, was significantly upregulated under white spot syndrome virus (WSSV) infection in the red swamp crayfish (Procambarus clarkii). Recombinant PcTrim significantly inhibited WSSV replication in crayfish. RNAi targeting PcTrim or blocking PcTrim with an antibody promoted WSSV replication in crayfish. Pulldown and co-IP assays showed that PcTrim can interact with the virus protein VP26. PcTrim restricts the expression level of dynamin, which is involved in the regulation of phagocytosis, by inhibiting AP1 entry into the nucleus. AP1-RNAi effectively reduced the expression levels of dynamin and inhibited host cell endocytosis of WSSV in vivo. Our study demonstrated that PcTrim might reduce early WSSV infection by binding to VP26 and then inhibiting AP1 activation, resulting in reduced endocytosis of WSSV in crayfish hemocytes.

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A Litopenaeus vannamei TRIM32 gene is involved in oxidative stress response and innate immunity

Cited by 12 publications

References 29 publications

Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection

Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection

Macroalgae Improve the Growth and Physiological Health of White Shrimp (Litopenaeus vannamei)

PcTrim prevents early infection with white spot syndrome virus by inhibiting AP1-induced endocytosis

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