A liver-specific lncRNA, FAM99B, suppresses hepatocellular carcinoma progression through inhibition of cell proliferation, migration, and invasion

Mo, Meile; Liu, Shun; Ma, Xiaoyun; Tan, Chao; Wei, Liangjia; Sheng, Yonghong; Song, Yanye; Zeng, Xianmin; Huang, Dongping; Qiu, Xiaoqiang

doi:10.1007/s00432-019-02954-8

Cited by 23 publications

(21 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MicroRNA‐148a targets the Met/Snail signal pathway to inhibit the EMT process and metastasis of HCC cells . In addition, some liver‐specific lncRNAs have similar inhibition on HCC cell proliferation, migration, and invasion, such as LINC01093 and FAM99B . Based on the above studies and the findings of our study, we inferred that liver‐specific non‐coding RNAs could be vital to maintaining the function of liver cells, and act as a tumor suppressor in the occurrence and development of HCC.…”

Section: Discussionsupporting

confidence: 61%

“…reported that LINC01093, a novel liver‐specific lncRNA, inhibits the progression of HCC by interacting with insulin‐like growth factor 2 mRNA‐binding protein 1 to promote GLI1 mRNA decay . In our previous study, we showed that a liver‐specific lncRNA, FAM99B, could serve as an antioncogene and provide a promising therapy target for HCC patients . These findings of liver‐specific non‐coding RNAs provide novel insights into the molecular mechanism of HCC occurrence and development.…”

Section: Introductionmentioning

confidence: 88%

See 1 more Smart Citation

LINC02499, a novel liver‐specific long non‐coding RNA with potential diagnostic and prognostic value, inhibits hepatocellular carcinoma cell proliferation, migration, and invasion

Tan

et al. 2020

Hepatology Research

Self Cite

View full text Add to dashboard Cite

Aim Liver‐specific non‐coding RNAs have been reported to play crucial roles in hepatocellular carcinoma (HCC). We investigated the possible biological performance of a novel liver‐specific long non‐coding RNA, LINC02499, in HCC. Methods The association between LINC02499 expression and HCC was evaluated based on The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, and then confirmed in a HCC cohort by quantitative real‐time polymerase chain reaction. The effects of LINC02499 on HCC cells were verified by gain‐ and loss‐of‐function assays. Pathway enrichment analyses were used to explore the potential mechanism of LINC02499 in HCC. Results LINC02499 expression was remarkably decreased in HCC tissues compared to adjacent non‐tumor tissues based on TCGA (P < 0.001) and GEO databases (P < 0.001) and our HCC cohort (P < 0.001). Decreased LINC02499 was also significantly associated with poorer overall survival in both the TCGA database (P = 0.009) and our HCC cohort (P = 0.002). Furthermore, the receiver operating characteristic analysis indicated that LINC02499 showed a good performance in HCC diagnosis (area under the curve = 0.879, P < 0.001), and both sensitivity and specificity were 83.8%. In addition, up‐ and downregulated LINC02499 significantly impacted proliferation, migration, and invasion abilities of HCC cells in vitro. Pathway enrichment analyses revealed that the potential target genes of LINC02499 were involved in “Complement and coagulation cascades” and “Butanoate metabolism” pathways. Conclusion LINC02499 could be a potential novel diagnostic and prognostic biomarker for HCC patients, and it could exert a tumor suppressor role in the progression of HCC.

show abstract

Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 88%

LINC02499, a novel liver‐specific long non‐coding RNA with potential diagnostic and prognostic value, inhibits hepatocellular carcinoma cell proliferation, migration, and invasion

Tan

et al. 2020

Hepatology Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…45 Mo et al have proved that the overexpression of FAM99B significantly inhibits the proliferation, migration, and invasion of HCC cells. 49 Zhuang et al have demonstrated that DRHC suppresses the proliferation, migration, and invasion of HCC cells. 50 In this study, we found that the upregulation of RMRP inhibited the proliferation, migration and invasion, and induced the apoptosis of HCC cells.…”

Section: Discussionmentioning

confidence: 99%

<p>Long Noncoding RNA RMRP Suppresses the Tumorigenesis of Hepatocellular Carcinoma Through Targeting microRNA-766</p>

Shao

Liu

Zhang

et al. 2020

OTT

View full text Add to dashboard Cite

Purpose: This study aimed to explore the regulatory effect of long noncoding RNA (lncRNA) ribonuclease mitochondrial RNA processing gene (RMRP) on hepatocellular carcinoma (HCC). Methods: The expression of RMRP in HCC tissues and cell lines was assessed by qRT-PCR. Kaplan-Meier method was utilized to analyze the correlation between RMRP expression and the survival of HCC patients. MHCC97H and HuH7 cells were transfected with pcDNA3.1-RMRP or pcDNA3.1, respectively. MTT and flow cytometry assays were conducted to examine the proliferation and apoptosis of HCC cells, respectively. The migration and invasion of HCC cells were assessed using wound healing and transwell assays, respectively. StarBase3.0 and dual-luciferase reporter gene assay were used to identify the target relationship between miR-766 and RMRP. A xenografted tumor model was established in rats to evaluate the effect of RMRP in vivo. Results: RMRP was down-regulated in HCC tissues and cells. Low expression of RMRP was correlated with poor survival of HCC patients. The A495 value and colony number were significantly decreased in pcDNA3.1-RMRP-transfected MHCC97H and HuH7 cells. The apoptosis rate was significantly increased in pcDNA3.1-RMRP-transfected MHCC97H and HuH7 cells. The migration rate and the number of invasive cells were significantly decreased in pcDNA3.1-RMRP-transfected MHCC97H and HuH7 cells. MiR-766 was a target of RMRP and eliminated the anti-tumor effect of RMRP on MHCC97H cells. The up-regulation of RMRP suppressed the growth of xenograft tumors in rats. Conclusion: Overexpression of RMRP suppressed the tumorigenesis of HCC by targeting miR-766.

show abstract

“…Among them, we observed a high degree of expression consistency between FAM99A and FAM99B (Family With Sequence Similarity 99 Member B). Regarding FAM99B, only one article was reported by searching that FAM99B is also a liver-specific lncRNA, which can inhibit cell proliferation, migration, and invasion of cells [22]. Such cellular function attribute may also be involved in the role of FAM99A in hepatic tumorigenesis and progression.…”

Section: Discussionmentioning

confidence: 99%

Identification of lncRNA FAM99A gene as a prognostic biomarker of hepatocellular carcinoma

Sun¹,

Lv²,

Zhong³

2020

Preprint

View full text Add to dashboard Cite

Background The complicated pathogenesis of hepatic cancer involves multiple clinical prognosis-associated oncogenes. Methods We utilized the bioinformatics approach to analyze the data from hepatic cancer cases collected by TCGA repository. Results We first found that the FAM99A (Family With Sequence Similarity 99 Member A) gene, a long non-coding RNA (lncRNA), is lowly expressed in hepatocellular carcinoma and closely related to clinical prognosis. We further analyzed the underling molecular mechanism from the perspectives of copy number variation (CNV), DNA methylation, immune cell infiltration, and related cellular pathway. Even though we did not observe a strong correlation between the FAM99A expression and the CNV or immune cell infiltration, the high methylation levels of the five methylated probe sites (cg24218935, cg01745044, cg04353359, cg04938738, cg25356611) were found to be negatively correlated with low expression level of FAM99A. Besides, we performed the enrichment analysis to screen out a group of FAM99A-correlated genes and molecular pathways, such as complement cascade, RNA metabolism, drug metabolic process, PPAR signaling pathway, or cell cycle. Conclusions The liver-specific FAM99A gene was first identified as a prognosis marker of hepatocellular carcinoma, and the underlying molecular mechanism involves DNA methylation and a series of cellular pathways.

show abstract

A liver-specific lncRNA, FAM99B, suppresses hepatocellular carcinoma progression through inhibition of cell proliferation, migration, and invasion

Cited by 23 publications

References 32 publications

LINC02499, a novel liver‐specific long non‐coding RNA with potential diagnostic and prognostic value, inhibits hepatocellular carcinoma cell proliferation, migration, and invasion

LINC02499, a novel liver‐specific long non‐coding RNA with potential diagnostic and prognostic value, inhibits hepatocellular carcinoma cell proliferation, migration, and invasion

<p>Long Noncoding RNA RMRP Suppresses the Tumorigenesis of Hepatocellular Carcinoma Through Targeting microRNA-766</p>

Identification of lncRNA FAM99A gene as a prognostic biomarker of hepatocellular carcinoma

Contact Info

Product

Resources

About