A Living Eukaryotic Autocementation Kit from Surface Display of Silica Binding Peptides on Yarrowia lipolytica

Abstract

With the development of civil engineering, the demand for suitable cementation materials is increasing rapidly. However, traditional cementation methods are not eco-friendly enough and more sustainable approach such as biobased cementation is required. To meet such demand, Euk.cement, a living eukaryotic cell-based biological autocementation kit, was created in this work. Through the surface display of different silica binding peptides on the fungus Yarrowia lipolytica, Euk.cement cells can immobilize onto any particles with a silica containing surface with variable binding intensity. Meanwhile, recombinant MCFP3 released from the cells will slowly consolidate this binding of cells to particles. The metabolism of immobilized living cells will finally complete the carbonate sedimentation and tightly stick the particles together. The system is designed to be initiated by blue light, making it controllable. This autocementation kit can be utilized for industrial and environmental applications that fit our concerns on making the cementation process eco-friendly.

A Bi‐Layer Hydrogel Cardiac Patch Made of Recombinant Functional Proteins

“…Additionally, the abundant electrons of Dopa and its susceptibility to oxidation might lead to higher degrees of cross-linking within A35m hydrogel. [40,41] The dense porous A35m hydrogels may serve as a better drug-loading scaffold for prolonged delivery than ASP materials.…”

Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement

“…Euk.cement is expected to stabilize underwater sands by biologically induced autocementation, for civil engineering or environmental restoration purposes. Surface-display and secretion of recombinant peptides and proteins by GM Y. lipolytica cells lead firstly to their immobilization onto silica sand particles and secondly to carbonate sedimentation, namely autocementation [106]. The Euk.cement kit project has received a gold medal at iGEM (International Genetically Engineered Machine) Competition in 2015 (MIT, Cambridge, MA, USA-http://2015.igem.org/Team:HUST-China (accessed on 3 June 2021)) but is not, to the best of our knowledge, commercialized yet.…”

Yarrowia lipolytica strains and their biotechnological applications: how natural biodiversity and metabolic engineering could contribute to cell factories improvement

“…Euk.cement is expected to stabilize underwater sands by biologically induced autocementation, for civil engineering or environmental restoration purposes. Surface-display and secretion of recombinant peptides and proteins by GM Y. lipolytica cells lead firstly to their immobilization onto silica sand particles and secondly to carbonate sedimentation, namely autocementation [89]. The Euk.cement kit project has received a gold medal at iGEM (International Genetically Engineered Machine) Competition in 2015 (MIT, USA -http://2015.igem.org/Team:HUST-China) but is not, to the best of our knowledge, commercialized yet.…”