A living system on a chip

Baker, Monya

doi:10.1038/471661a

Cited by 186 publications

(148 citation statements)

References 10 publications

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“…3D imaging generates more accurate results on the shape, size, location and relationship between cellular structures or processes. An even more complex acquisition technology includes the "organ on a chip approach," which uses a 3D microfluidic cell culture chip that can both measure and simulate the activities, mechanics and physiological responses of entire organs or organ systems (Huh et al, 2011;Baker, 2011). Using this approach, multiple biological processes, e.g., in the liver, kidney, lung or heart can be stimulated (pharmacologically or mechanically) during the imaging data acquisition, which allows the investigation of biological mechanisms and responses in organ specific microenvironments (Yum et al, 2014).…”

Section: Current High Content Imaging Applications In Safety Sciencesmentioning

confidence: 99%

Current approaches and future role of high content imaging in safety sciences and drug discovery

Vliet

Daneshian

Beilmann

et al. 2014

ALTEX

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* a report of t 4 -the transatlantic think tank for toxicology, a collaboration of the toxicologically oriented chairs in Baltimore, Konstanz and Utrecht sponsored by the Doerenkamp-Zbinden Foundation; participants do not represent their institutions and do not necessarily endorse all recommendations made.Disclaimer: This document has been reviewed by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. Summary High content imaging combines automated microscopy with image analysis approaches to simultaneously quantify multiple phenotypic and/or functional parameters in biological systems. The technology has become an important tool in the fields of safety sciences and drug discovery, because it can be used for mode-of-action identification, determination of hazard potency and the discovery of toxicity targets and biomarkers. In contrast to conventional biochemical endpoints, high content imaging provides insight into the spatial distribution and dynamics of responses in biological systems

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Section: Current High Content Imaging Applications In Safety Sciencesmentioning

confidence: 99%

Current approaches and future role of high content imaging in safety sciences and drug discovery

Vliet

Daneshian

Beilmann

et al. 2014

ALTEX

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“…For example, it has enabled the control and analysis of chemical reactions by infusing multiple sub-strates into nely processed microsized channels [2,3] and direct culturing of cells within micro uidic devices [4,5]. We have developed and reported a culturing device that generates oxygen gradients in cell culture layers [6].…”

Section: Introductionmentioning

confidence: 99%

Immobilized Monolayer Nanoparticles in a Microfluidic Device for Surface Enhanced Raman Scattering Measurement

Hase

Ishigaki

Shibusawa

et al. 2017

ABE

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Micro uidic devices are powerful bioapplication tools for cellular experiments in particular, as they can regulate physical and chemical parameters such as the ow rate, shear stress, oxygen, and molecular concentrations in a culture medium to mimic physiological and pathological microenvironments in vitro. However, as cell cultures take place in enclosed spaces, culture samples have to be removed repeatedly to monitor cell-derived metabolites and proteins in order to maintain the cellular microenvironment. We report a simple method for obtaining surface enhanced Raman scattering (SERS) spectra through self-assembly of a nanoparticle monolayer on polydimethylsiloxane (PDMS), which is commonly used in highly biocompatible micro uidic devices. Silica nanoparticles were stabilized as a hexagonal close packed structure on an O 2 plasma-processed PDMS membrane, and was coated with silver using vapor deposition to create an SERS plate. When this SERS plate was installed in a micro uidic device, the nanoparticles did not peel off even after long-term uid immersion. The SERS spectra exhibited stable SERS generation and an enhancement factor of more than 1.5 × 10 6 of the Raman signals from rhodamine 6G compared to the signals without nanoparticles. The SERS spectra of lactate and ATP were obtained, and Raman shifts due to the different masses of 12 C-and 13 C-lactate were observed, suggesting that the proposed method can be applied to determine the cellular metabolic ux in micro uidic devices. We also obtained cell membrane-derived SERS spectra by culturing murine mammary carcinoma 4T1 cells directly on the nanoparticle membrane. PDMS has high biocompatibility and is often used for fabricating micro uidic devices. Through tight binding of the nanoparticles to the O 2 plasma-treated PDMS, the chemical reaction products or the metabolites from cells can be measured for a long duration, while maintaining the microenvironment. We anticipate that this technology can be utilized as a useful tool for analyzing cellular metabolic functions and imaging cellular distributions without staining in various pathological models created in micro uidic devices.

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“…Therefore a deeper understanding of cell response on oxygen limitation is of particular interest in medical basic research. For this issue lab-ona-chip (LOC) devices have become more and more important for in-vitro cell cultivation [6]. Especially perfused LOCs are able to mimic physiological environment inducing mechanical stimulation and convective mass transfer.…”

Section: Introductionmentioning

confidence: 99%

Hypoxia-on-a-chip

Busek

Grünzner

Steege

et al. 2016

Current Directions in Biomedical Engineering

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Abstract:In this work a microfluidic cell cultivation device for perfused hypoxia assays as well as a suitable controlling unit are presented. The device features active components like pumps for fluid actuation and valves for fluid direction as well as an oxygenator element to ensure a sufficient oxygen transfer. It consists of several individually structured layers which can be tailored specifically to the intended purpose. Because of its clearness, its mechanical strength and chemical resistance as well as its well-known biocompatibility polycarbonate was chosen to form the fluidic layers by thermal diffusion bonding. Several oxygen sensing spots are integrated into the device and monitored with fluorescence lifetime detection. Furthermore an oxygen regulator module is implemented into the controlling unit which is able to mix different process gases to achieve a controlled oxygenation. First experiments show that oxygenation/deoxygenation of the system is completed within several minutes when pure nitrogen or air is applied to the oxygenator. Lastly the oxygen input by the pneumatically driven micro pump was quantified by measuring the oxygen content before and after the oxygenator.

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A living system on a chip

Cited by 186 publications

References 10 publications

Current approaches and future role of high content imaging in safety sciences and drug discovery

Current approaches and future role of high content imaging in safety sciences and drug discovery

Immobilized Monolayer Nanoparticles in a Microfluidic Device for Surface Enhanced Raman Scattering Measurement

Hypoxia-on-a-chip

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