A LncRNA-miRNA-mRNA ceRNA regulatory network based tuberculosis prediction model

Feng, Jiaxuan; Bian, Qin; He, Xianwei; Zhang, Han; He, Jiujiang

doi:10.1016/j.micpath.2021.105069

Cited by 7 publications

(5 citation statements)

References 30 publications

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“…Therefore, the physical interaction of ceRNA-miRNAs leads to a complex regulatory network of ceRNA/miRNA/mRNA that controls gene expression at the transcriptional, post-transcriptional, and post-translational levels [ 16 , 17 ]. Some studies have found that these interacting ceRNA/miRNA/mRNA networks are involved in many cellular processes and human diseases, including tumorigenesis [ 18 , 19 , 20 ]. The ceRNA/mRNA network has also been reported in the study of breast cancer mechanisms.…”

Section: Introductionmentioning

confidence: 99%

HOTAIR/miR-203/CAV1 Crosstalk Influences Proliferation, Migration, and Invasion in the Breast Cancer Cell

Shi

Chen

Wang

et al. 2022

IJMS

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In recent years, malignant breast cancer metastasis has caused a great increase in mortality. Research on the genetic and molecular mechanisms of malignant breast cancer has continued to deepen, and targeted therapy has become the general trend. Among them, competing endogenous RNA (ceRNA)-related molecules have received much attention. Homeobox transcript antisense RNA (HOTAIR) has been reported to function extensively as a ceRNA in breast cancer. Notably, miR-203 and Caveolin 1 (CAV1) have also been found to play a role in breast cancer. However, the relationship between the three remains unclear. In this study, we present a new mechanic through bioinformatics tool and basic experiments: the HOTAIR/miR-203/CAV1 axis, which complemented the role network of HOTAIR as a ceRNA, thus, it will provide a novel potential idea for breast cancer research and therapy.

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Section: Introductionmentioning

confidence: 99%

HOTAIR/miR-203/CAV1 Crosstalk Influences Proliferation, Migration, and Invasion in the Breast Cancer Cell

Shi

Chen

Wang

et al. 2022

IJMS

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“…Significant progress has been made toward the understanding of the mechanistic and functional roles of lncRNAs across species and various cell types. They play crucial roles in transcription and translation regulation, RNA metabolism, epigenetic control and signaling through cis interactions with RNAs, and through trans interactions with DNA, RNAs, or proteins [53][54][55]. Based on the accumulation of genomic knowledge and the expanding lncRNA repository, the identification and functional verification of lncRNAs in phytopathogens were also conducted [56,57].…”

Section: Discussionmentioning

confidence: 99%

The Identification and Comparative Analysis of Non-Coding RNAs in Spores and Mycelia of Penicillium expansum

Lai,

Yu,

Pan

et al. 2023

JoF

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Penicillium expansum is the most popular post-harvest pathogen and causes blue mold disease in pome fruit and leads to significant economic losses worldwide every year. However, the fundamental regulation mechanisms of growth in P. expansum are unclear. Recently, non-coding RNAs (ncRNAs) have attracted more attention due to critical roles in normalizing gene expression and maintaining cellular genotypes in organisms. However, the research related to ncRNAs in P. expansum have not been reported. Therefore, to provide an overview of ncRNAs on composition, distribution, expression changes, and potential targets in the growth process, a comparative transcriptomic analysis was performed on spores and mycelia of P. expansum in the present study. A total of 2595 novel mRNAs, 3362 long non-coding RNAs (lncRNAs), 10 novel microRNAs (miRNAs), 86 novel small interfering RNAs (siRNAs), and 11,238 circular RNAs (circRNAs) were predicted and quantified. Of these, 1482 novel mRNAs, 5987 known mRNAs, 2047 lncRNAs, 40 miRNAs, 38 novel siRNAs, and 9235 circRNAs were differentially expressed (DE) in response to the different development stages. Afterward, the involved functions and pathways of DE RNAs were revealed via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database enrichment analysis. The interaction networks between mRNAs, lncRNAs, and miRNAs were also predicted based on their correlation coefficient of expression profiles. Among them, it was found that miR168 family members may play important roles in fungal growth due to their central location in the network. These findings will contribute to a better understanding on regulation machinery at the RNA level on fungal growth and provide a theoretical basis to develop novel control strategies against P. expansum.

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“…It is well known that the most typical regulatory mechanism of lncRNAs is to act as miRNAs sponges, negatively modulating the miRNAs expressions. [20,21] Starbase predicted that SNHG14 targeted miR-497a-5p (Figure 3A). Thereafter, miR-497a-5p was overexpressed in adipocytes, and miR-497a-5p overexpression efficiency was further verified (Figure 3B).…”

Section: Snhg14 Targets Mir-497a-5pmentioning

confidence: 99%

LncRNA SNHG14/miR‐497a‐5p/BACE1 axis modulates obesity‐induced adipocyte inflammation and endoplasmic reticulum stress

Han

Tian

Wang

et al. 2023

J Biochem & Molecular Tox

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Obesity is a metabolic disease with excess weight. LncRNA SNHG14 is abnormally expressed in numerous diseases. This research aimed to enucleate the lncRNA SNHG14 role in obesity. Adipocytes were treated with free fatty acid (FFA) to establish an in vitro model for obesity. Mice were fed a high-fat diet to construct an in vivo model. Gene levels were determined using quantitative real-time PCR (RT-PCR). The protein level was checked by western blot. The lncRNA SNHG14 role in obesity was assessed using western blot and enzyme-linked immunosorbent assay. The mechanism was estimated by Starbase, dual-luciferase reporter gene assay, and RNA pull-down. LncRNA SNHG14 function in obesity was estimated using mouse xenograft models, RT-PCR, western blot, and enzyme-linked immunosorbent assay. LncRNA SNHG14 and BACE1 levels were increased, but the miR-497a-5p level was decreased in FFA-induced adipocytes. Interference with lncRNA SNHG14 reduced endoplasmic reticulum (ER) stress-related molecules GRP78 and CHOP expressions in FFA-induced adipocytes, and decreased IL-1β, IL-6, and TNF-α expressions, indicating that lncRNA SNHG14 knockdown mitigated FFA-induced ER stress and inflammation in adipocytes. Mechanistically, lncRNA SNHG14 combined with miR-497a-5p, and miR-497a-5p targeted BACE1. Meanwhile, lncRNA SNHG14 knockdown reduced levels of GRP78, CHOP, IL-1β, IL-6, and TNF-α, while cotransfection with anti-miR-497a-5p or pcDNA-BACE1 abolished these trends. Rescue assays illustrated that lncRNA SNHG14 knockdown relieved FFA-induced adipocyte ER stress and inflammation through miR-497a-5p/BACE1. Meanwhile, lncRNA SNHG14 knockdown restrained adipose inflammation and ER stress caused by obesity in vivo. LncRNA SNHG14 mediated obesity-induced adipose inflammation and ER stress through miR-497a-5p/BACE1.

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A LncRNA-miRNA-mRNA ceRNA regulatory network based tuberculosis prediction model

Cited by 7 publications

References 30 publications

HOTAIR/miR-203/CAV1 Crosstalk Influences Proliferation, Migration, and Invasion in the Breast Cancer Cell

HOTAIR/miR-203/CAV1 Crosstalk Influences Proliferation, Migration, and Invasion in the Breast Cancer Cell

The Identification and Comparative Analysis of Non-Coding RNAs in Spores and Mycelia of Penicillium expansum

LncRNA SNHG14/miR‐497a‐5p/BACE1 axis modulates obesity‐induced adipocyte inflammation and endoplasmic reticulum stress

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