A Localized Surface Plasmon Resonance Sensing Method for Simultaneous Determination of Atenolol and Amiloride in Pharmaceutical Dosage Forms and Urine Samples

Abstract

This contribution describes a simple, fast, and sensitive application of localized surface plasmon resonance effect of silver nanoparticles for simultaneous determination of antihypertensive drugs' mixture atenolol and amiloride in both pharmaceutical dosage forms and in biological samples (urine). Silver nanoparticles were prepared by chemical reduction of silver nitrate using hydroxylamine HCL in an alkaline medium. Application of silver-hydroxylamine nanoparticles (SH NPs) provides many advantages including reproducibility, sensitivity, and cost effective way of analyte determination. Amiloride has four amino groups which act as attachment points on the surface of silver nanoparticles resulting in a synergistic effect on the absorption intensity of atenolol, leading to increase the sensitivity of the determination of both compounds. This method shows excellent advantages comparing with the previously reported methods, including accuracy, precision, and selectivity. The linear range of atenolol is 1 × 10−5–1 × 10−4 mol·L−1 and of amiloride is 1 × 10−6–1 × 10−5 mol·L−1. The limit of detection (LOD) values of atenolol and amiloride are 0.89 × 10−5 and 0.42 × 10−6 mol·L−1, respectively.

Utilization of Localized Surface Plasmon Resonance of Silver Nanoparticles for the Spectrofluorimetric Estimation of Oxymetazoline in Dosage Forms: Application to Aqueous Humor

“…Metal induced uorescence enhancement phenomenon is proposed to be due to interactions of the excited uorophores with surface plasmon resonances in metals. In this study, the interactions are suggested to be between AgNPs and nitrogen atoms of the imidazoline ring of OMZ [5,6] leading to remarkable enhancement of OMZ uorescence intensity, as illustrated in Schem.1.…”

“…The incident photon may merge with the metal electrons and start to oscillate coherently [3]. Recent investigations have con rmed that LSPR is an in uential technique in biomedical applications due to its sensitivity to material type, size and dielectric constant [4,5], in addition to its impact on enhancing sensitivity of different spectroscopic techniques including uorescence [6].…”

Effect of Methadone and Tramadol Opioids on Stem Cells Based on Integrated Plasmonic-Ellipsometry Technique

“…12,13 The application of plasmonics in pharmaceutics allows SPR biosensors to detect molecular binding activities at low concentrations as well as cellular changes. 14 El-Zahry 15 has utilized localized surface plasmon resonance (LSPR) of Ag nanoparticles for parallel detection of the mixture of antihypertensive drugs of atenolol and amiloride pharmaceutical dosage forms as well as urine samples. Their method was based on the UV absorption intensities of these mixed analytes.…”