A long-lasting guided bone regeneration membrane from sequentially functionalised photoactive atelocollagen

He, Liang; Yin, Jie; Man, Kenny; Yang, Xuebin; Calciolari, Elena; Donos, Nikolaos; Russell, Stephen J.; Wood, David; Tronci, Giuseppe

doi:10.1016/j.actbio.2021.12.004

Cited by 20 publications

(18 citation statements)

References 74 publications

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“…Pristine collagen usually degraded fast in vivo, restricting its further utilization in tissue engineering 43 . As shown in Table 4, the enzyme degraded degree of collagen hydrogels decreased from 75.3% to 27.9% with increasing laponite concentration from 0% to 10%.…”

Section: Resultsmentioning

confidence: 98%

Infiltration of laponite: An effective approach to improve the mechanical properties and thermostability of collagen hydrogel

Tian

Yang

et al. 2022

J of Applied Polymer Sci

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Rapid aggregate precipitation of collagen and laponite in solution restricted the development of corresponding hybrid materials. Herein, a facile method of immerging collagen hydrogel into laponite dispersion was adopted to prepare hybrid collagen/laponite hydrogel, avoiding the precipitation. Collagen hydrogel shrunk and water content decreased dramatically after laponite solution treatment. According to the images of Scanning electron microscopy and energy dispersive system, the uniformly dispersed laponite in collagen hydrogel was observed, demonstrating infiltration of laponite into collagen hydrogel. X-ray photoelectron spectroscopy results revealed that coordination bonds generated between magnesium in laponite and collagen. Thermogravimetric analysis and Differential scanning calorimetry results displayed thermal decomposition temperature of collagen hydrogel increased from 321 to 342 C, and denaturation temperature rose from 43.8 to 50.8 C after laponite infiltration. Rheological and compressive tests showed the elastic modulus, fracture stress, compressive modulus and rupture point of hydrogel treated by 10% laponite were 695.2 Pa, 253.3 kPa, 13.5 kPa and 76.7%, respectively, which were about 3.5, 5.5, 45.0, and 1.4 times larger than those of pristine collagen hydrogel. Besides, enzyme degraded rate of collagen was decelerated, and the hydrogel was still nontoxic with the infiltration of laponite. The promising data demonstrated infiltration of laponite was an effective way to fabricate collagen/laponite hybrid materials with desirable properties.

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Section: Resultsmentioning

confidence: 98%

Infiltration of laponite: An effective approach to improve the mechanical properties and thermostability of collagen hydrogel

Tian

Yang

et al. 2022

J of Applied Polymer Sci

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“…For the purposes of spinal cord tissue regeneration and neural cell encapsulation, GMA was chosen as the linker moiety over other previously investigated moieties, such as 4-vinylbenzyl chloride (4VBC) and methacrylic anhydride (MA) [ 30 , 31 , 48 , 49 , 50 ], due to its greater elasticity, resulting in hydrogels with greater compressibility and reduced compressive modulus [ 30 , 31 ]. As such, it was rationalised GMA would produce hydrogels closer to the desired shear modulus.…”

Section: Discussionmentioning

confidence: 99%

A Biomimetic Nonwoven-Reinforced Hydrogel for Spinal Cord Injury Repair

et al. 2022

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In clinical trials, new scaffolds for regeneration after spinal cord injury (SCI) should reflect the importance of a mechanically optimised, hydrated environment. Composite scaffolds of nonwovens, self-assembling peptides (SAPs) and hydrogels offer the ability to mimic native spinal cord tissue, promote aligned tissue regeneration and tailor mechanical properties. This work studies the effects of an aligned electrospun nonwoven of P11-8—enriched poly(ε-caprolactone) (PCL) fibres, integrated with a photo-crosslinked hydrogel of glycidylmethacrylated collagen (collagen-GMA), on neurite extension. Mechanical properties of collagen-GMA hydrogel in compression and shear were recorded, along with cell viability. Collagen-GMA hydrogels showed J-shaped stress–strain curves in compression, mimicking native spinal cord tissue. For hydrogels prepared with a 0.8-1.1 wt.% collagen-GMA concentration, strain at break values were 68 ± 1–81 ± 1% (±SE); maximum stress values were 128 ± 9–311 ± 18 kPa (±SE); and maximum force values were 1.0 ± 0.1–2.5 ± 0.1 N (±SE). These values closely mimicked the compression values for feline and porcine tissue in the literature, especially those for 0.8 wt.%. Complex shear modulus values fell in the range 345–2588 Pa, with the lower modulus hydrogels in the range optimal for neural cell survival and growth. Collagen-GMA hydrogel provided an environment for homogenous and three-dimensional cell encapsulation, and high cell viability of 84 ± 2%. Combination of the aligned PCL/P11-8 electrospun nonwoven and collagen-GMA hydrogel retained fibre alignment and pore structure, respectively, and promoted aligned neurite extension of PC12 cells. Thus, it is possible to conclude that scaffolds with mechanical properties that both closely mimic native spinal cord tissue and are optimal for neural cells can be produced, which also promote aligned tissue regeneration when the benefits of hydrogels and electrospun nonwovens are combined.

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“…The accelerated degradation test further revealed that the percolated struvite actively aided membrane degradation. Considering that the repair of a critical-sized defect can take up to 6 months, [10,13] the results of in vitro degradation suggest that the struvite-doped membranes can serve as a long-lasting barrier to maintain the isolated capsule for large-area bony reconstruction.…”

Section: In Vitro Degradation Profiles Of the Bilayer Membranesmentioning

confidence: 99%

“…The uncontrollable degradation of collagen‐based membranes often leads to early collapse and barrier failure. [ 10 ] More critically, the current GBR products are mostly designed as a strong physical hindrance, whereas show limited bioactivity. [ 11 ] Hence, researchers are continuously investigating the development of an active membrane with superior barrier performance, favorable bioresorption kinetics, and outstanding therapeutic efficacy.…”

Section: Introductionmentioning

confidence: 99%

A Bilayer Membrane Doped with Struvite Nanowires for Guided Bone Regeneration

Zhu

Zhou

Dai

et al. 2022

Adv Healthcare Materials

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Guided bone regeneration (GBR) therapy demonstrates a prominent curative effect on the management of craniomaxillofacial (CMF) bone defects. In this study, a GBR membrane consisting of a microporous layer and a struvite‐nanowire‐doped fibrous layer is constructed via non‐solvent induced phase separation, followed by an electrospinning procedure to treat critical‐sized calvarial defects. The microporous layer shows selective permeability for excluding the rapid‐growing non‐osteogenic tissues and potential wound stabilization. The nanowire‐like struvite is synthesized as the deliverable therapeutic agent within the fibrous layer to facilitate bone regeneration. Such a membrane displays a well‐developed heterogeneous architecture, satisfactory mechanical performance, and long‐lasting characteristics. The in vitro biological evaluation reveals that apart from being a strong barrier, the bilayer struvite‐laden membrane can actively promote cellular adhesion, proliferation, and osteogenic differentiation. Consequently, the multifunctional struvite‐doped membranes are applied to treat 5 mm‐sized bilateral calvarial defects in rats, resulting in overall improved healing outcomes compared with the untreated or the struvite‐free membrane‐treated group, which is characterized by enhanced osteogenesis and significantly increased new bone formation. The encouraging preclinical results reveal the great potential of the bilayer struvite‐doped membrane as a clinical GBR device for augmenting large‐area CMF bone reconstruction.

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A long-lasting guided bone regeneration membrane from sequentially functionalised photoactive atelocollagen

Cited by 20 publications

References 74 publications

Infiltration of laponite: An effective approach to improve the mechanical properties and thermostability of collagen hydrogel

Infiltration of laponite: An effective approach to improve the mechanical properties and thermostability of collagen hydrogel

A Biomimetic Nonwoven-Reinforced Hydrogel for Spinal Cord Injury Repair

A Bilayer Membrane Doped with Struvite Nanowires for Guided Bone Regeneration

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