A long-term depression of AMPA currents in cultured cerebellar purkinje neurons

Linden, David J.; Dickinson, Michael H.; Smeyne, Michelle; Connor, John A.

doi:10.1016/0896-6273(91)90076-c

Cited by 389 publications

(161 citation statements)

References 42 publications

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“…It was reported that, at the PF3 Purkinje cell synapse, the simultaneous activation of AMPAR and mGluR is necessary for the induction of LTD (Kano and Kato, 1988;Linden et al, 1991;Hartell, 1994;Hemart et al, 1995). Indeed, LTD is absent in mGluR1-deficient mice (Aiba et al, 1994;Conquet et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Cross Talk between Metabotropic and Ionotropic Glutamate Receptor-Mediated Signaling in Parallel Fiber-Induced Inositol 1,4,5-Trisphosphate Production in Cerebellar Purkinje Cells

Okubo¹,

Kakizawa²,

Hirose³

et al. 2004

J. Neurosci.

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In many excitatory glutamatergic synapses, both ionotropic glutamate receptors (iGluRs) and metabotropic glutamate receptors (mGluRs) are closely distributed on the postsynaptic membrane. However, the functional significance of the close distribution of the two types of glutamate receptors has not been fully clarified. In this study, we examined the functional interaction between iGluR and mGluR at parallel fiber (

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Section: Discussionmentioning

confidence: 99%

Cross Talk between Metabotropic and Ionotropic Glutamate Receptor-Mediated Signaling in Parallel Fiber-Induced Inositol 1,4,5-Trisphosphate Production in Cerebellar Purkinje Cells

Okubo¹,

Kakizawa²,

Hirose³

et al. 2004

J. Neurosci.

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“…Electrophysiological studies support the presence of non-NMDA receptors at the parallel fiber/Purkinje spine synapse and the involvement of AMPA receptors in longterm depression (LTD; reviews by Crepe1 and Audinat, 1991;Linden et al, 1991;Linden, 1994). However, it is not clear which AMPA subunits are present in the postsynaptic membrane of the parallel fiber/Purkinje spine synapse.…”

Section: Physiological Signijicancementioning

confidence: 99%

Immunoprecipitation, immunoblotting, and immunocytochemistry studies suggest that glutamate receptor delta subunits form novel postsynaptic receptor complexes

et al. 1995

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An antibody was made to a C-terminus peptide of the glutamate receptor delta 2 subunit and used to study the distribution, biochemical properties, and developmental expression of the delta receptor in rat brain. The antibody recognizes both delta 1 and delta 2 but not AMPA, kainate, NMDA, and mGluR1 alpha glutamate receptor subunits based on Western blot analysis of transfected HEK-293 cells. Western blot analysis of brain showed a single immunoreactive band, migrating at M(r) = 114,000. Immunoprecipitation of detergent-solubilized cerebellar membranes was done to determine if delta is associated with other glutamate receptor subunits and if it binds any of the common excitatory amino acid ligands. Based on results of these studies, AMPA, kainate, NMDA, and mGluR1 alpha subunits do not coassemble with delta subunits, and 3H-glutamate, 3H-AMPA and 3H-kainate do not bind to the delta receptor complex. Western blot and immunocytochemical analyses showed marked expression of delta in the cerebellum while lower levels were detected in other regions of the brain. A dramatic increase of delta 1/2 immunoreactivity was observed in the cerebellum between the ages of 10 and 15 d postnatal. Light and electron microscopy, respectively, demonstrated dense immunostaining in Purkinje cells and in postsynaptic densities of the adult parallel fiber-Purkinje spine synapse. The prominent delta 1/2 immunoreactivity found in the parallel fiber-Purkinje spine synapse, and the temporal correlation of the development of this synapse with the major increase in delta 1/2 immunoreactivity, suggest a major functional role for the delta subunits in cerebellar circuitry.

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“…The PF-PN synapse has been a powerful system to explore many of these predictions. The first form of PF-PN long-term plasticity to be described on the basis of experimental data was "cerebellar LTD" that is based on a heterosynaptic mechanism (conjunctive climbing fiber and PF activity) and that is expressed as a reduced postsynaptic response to synaptically released PF transmitter [19,20,25]. In line with the theoretical quest to identify bidirectional plasticity mechanisms, postsynaptic expression of PF-PN LTP was discovered and characterized [11,23,24].…”

Section: Introductionmentioning

confidence: 99%

Presynaptically expressed long-term depression at cerebellar parallel fiber synapses

Qiu

Knöpfel

2008

Pflugers Arch - Eur J Physiol

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Plasticity at synapses between parallel fiber (PF) and Purkinje neurons (PN) is widely accepted as a cellular model for certain forms of cerebellar learning. Although PF-PN synapses are known to express bidirectional longterm plasticity at the postsynaptic site, long-term plasticity at the presynaptic site is currently limited to potentiation of the synapses. In this paper, we report on presynaptically expressed PF long-term depression (preLTD) that is observed when presynaptically expressed PF long-term potentiation (preLTP) is pharmacologically prevented. PF preLTD is most efficiently induced by 4 Hz PF stimulation and requires activation of cannabinoid CB1 receptors. Our results indicate that, during preLTD induction, endocannabinoids are released in an NMDA receptor-dependent, but not mGlu1 receptor-dependent, fashion. We conclude that bidirectional plasticity mechanisms exist for both presynaptic and postsynaptic components of cerebellar learning.

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A long-term depression of AMPA currents in cultured cerebellar purkinje neurons

Cited by 389 publications

References 42 publications

Cross Talk between Metabotropic and Ionotropic Glutamate Receptor-Mediated Signaling in Parallel Fiber-Induced Inositol 1,4,5-Trisphosphate Production in Cerebellar Purkinje Cells

Cross Talk between Metabotropic and Ionotropic Glutamate Receptor-Mediated Signaling in Parallel Fiber-Induced Inositol 1,4,5-Trisphosphate Production in Cerebellar Purkinje Cells

Immunoprecipitation, immunoblotting, and immunocytochemistry studies suggest that glutamate receptor delta subunits form novel postsynaptic receptor complexes

Presynaptically expressed long-term depression at cerebellar parallel fiber synapses

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