The patient safety culture based on the safety attitudes questionnaire from a state-owned regional teaching hospital in Taiwan is analyzed in terms of six dimensions by independent sample t test and one-way analysis of variance from the viewpoints of physicians and nurses. Gender, age, job position, job status, and education are critical demographic variables that have significant impacts on five out of six dimensions. In contrast, teamwork climate, safety climate, job satisfaction, and perceptions of management are the critical dimensions because they are statistically influenced by six out of nine demographic variables. To relentlessly improve the patient safety culture in this regional hospital, the hospital management needs to pay much attention to critical demographic variables, identify those employees with statistically lower perceptions in terms of dimensions, and then take actions to address their deficiencies.