A longitudinal study of Babesia and Theileria infections in cattle in Sri Lanka

Sivakumar, Thillaiampalam; Kothalawala, Hemal; Weerasooriya, Gayani; Silva, Seekkuge Susil Priyantha; Puvanendiran, S.; Munkhjargal, Tserendorj; Igarashi, Ikuo; Yokoyama, Naoki

doi:10.1016/j.vprsr.2016.11.009

Cited by 8 publications

(14 citation statements)

References 43 publications

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“…A series of molecular epidemiological surveys have been conducted in various countries to detect and genetically characterize Babesia parasites present in apparently healthy cattle [10,11,12,13]. Molecular methods based on the detection of Babesia DNA in blood by PCR techniques provides results with high sensitivity and specificity.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Tools for the Identification of Babesia sp. in Persistently Infected Cattle

2019

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Bovine babesiosis is a tick-borne disease of cattle caused by the protozoan parasites of the genus Babesia. Babesia bovis, Babesia bigemina and Babesia divergens are considered by International health authorities (OIE) as the principal species of Babesia that cause bovine babesiosis. Animals that recover from a babesial primo infection may remain as persistent carriers with no clinical signs of disease and can be the source of infection for ticks that are able to acquire Babesia parasites from infected cattle and to transmit Babesia parasites to susceptible cattle. Several procedures that have been developed for parasite detection and diagnosis of this infectious carrier state constitute the basis for this review: A brief description of the direct microscopic detection of Babesia-infected erytrocytes; PCR-based diagnostic assays, which are very sensitive particularly in detecting Babesia in carrier cattle; in-vitro culture methods, used to demonstrate presence of carrier infections of Babesia sp.; animal inoculation, particularly for B. divergens isolation are discussed. Alternatively, persistently infected animals can be tested for specific antibabesial antibodies by using indirect serological assays. Serological procedures are not necessarily consistent in identifying persistently infected animals and have the disadvantage of presenting with cross reactions between antibodies to Babesia sp.

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Section: Introductionmentioning

confidence: 99%

Diagnostic Tools for the Identification of Babesia sp. in Persistently Infected Cattle

2019

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“…A recent series of molecular epidemiological surveys was conducted in Sri Lanka to detect and genetically characterize hemoprotozoan parasites in apparently healthy cattle (31)(32)(33)(34)(35). In contrast, genetic techniques have never been used to investigate clinical cases of bovine babesiosis in Sri Lanka.…”

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Genetic Analysis of Babesia Isolates from Cattle with Clinical Babesiosis in Sri Lanka

et al. 2018

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Bovine babesiosis is a serious threat to the cattle industry. We prepared blood DNA samples from 13 cattle with clinical babesiosis from the Badulla ( = 8), Jaffna ( = 3), and Kilinochchi ( = 2) districts in Sri Lanka. These DNA samples tested positive in PCR assays specific for ( = 9), ( = 9), and ( = 1). Twelve cattle were positive for and/or One cow was negative for the tested species but was positive for on microscopic examination; the phylogenetic positions of 18S rRNA and cytochrome oxidase subunit III gene sequences suggested that the cow was infected with sp. Mymensingh, which was recently reported from a healthy cow in Bangladesh. We then developed a novel sp. Mymensingh-specific PCR assay and obtained positive results for one other sample. Analysis of gene sequences from the cow with positive -specific PCR results demonstrated that the animal was infected not with but with sp. Hue-1, which was recently reported from asymptomatic cattle in Vietnam. The virulence of sp. Hue-1 is unclear, as the cow was coinfected with and However, sp. Mymensingh probably causes severe clinical babesiosis, as it was the sole species detected in a clinical case. The present study revealed the presence of two bovine species not previously reported in Sri Lanka, plus the first case of severe bovine babesiosis caused by a species other than ,, and .

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“…bigemina rap - 1 (LC323168) gene sequences ( Table 1 ) [ 20 ]. The PCR products were ligated into a pGEX-4T1 ( B. bovis ) or pGEX-6p2 ( B. bigemina ) plasmid vector (GE Healthcare, Little Chalfont, U.K.), and recombinant RAP-1 antigens were expressed as glutathione S -transferase-fusion proteins as described previously [ 20 ]. Finally, the glutathione S -transferase tag was cleaved using thrombin and PreScission protease (GE Healthcare) to purify the B .…”

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confidence: 99%

“…bovis and B . bigemina recombinant RAP-1 proteins (rRAP-1), respectively [ 20 ]. These rRAP-1 antigens were then used to develop ELISAs for the sero-diagnosis of B .…”

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confidence: 99%

“…Finally, the optical density (OD) value was measured at 450 nm. A sample was considered positive if the OD value was higher than the cut-off value that had been determined as the sum of the mean OD values and 5 ×standard deviations of the five negative serum samples used in each plate (data not shown) [ 20 ]. Basic Local Alignment Search Tool (https://blast.ncbi.nlm.nih.gov/Blast.cgi) analysis showed that the Vietnamese B .…”

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Serological and molecular surveys of <i>Babesia bovis</i> and <i>Babesia bigemina</i> among native cattle and cattle imported from Thailand in Hue, Vietnam

Sivakumar

Lân

Long

et al. 2018

The Journal of Veterinary Medical Science

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Serum and DNA from blood samples collected from Vietnamese yellow cattle (n=101) and cattle imported from Thailand (n=54) at a Vietnamese slaughter house were screened for Babesia bovis and Babesia bigemina infections by enzyme-linked immunosorbent assay (ELISA) and PCR. The positive rates determined by ELISA (B. bovis and B. bigemina) or PCR (B. bigemina) in the Vietnamese cattle were significantly higher than those found in Thai cattle. Some PCR-positive Vietnamese animals were ELISA-negative, whereas all PCR-positive Thai cattle were ELISA-positive, suggesting that the animals were infected in Thailand. Importing Babesia-infected cattle may lead to the introduction of new parasite strains, possibly compromising the development of anti-Babesia immune control strategies in Vietnam.

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A longitudinal study of Babesia and Theileria infections in cattle in Sri Lanka

Cited by 8 publications

References 43 publications

Diagnostic Tools for the Identification of Babesia sp. in Persistently Infected Cattle

Diagnostic Tools for the Identification of Babesia sp. in Persistently Infected Cattle

Genetic Analysis of Babesia Isolates from Cattle with Clinical Babesiosis in Sri Lanka

Serological and molecular surveys of <i>Babesia bovis</i> and <i>Babesia bigemina</i> among native cattle and cattle imported from Thailand in Hue, Vietnam

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