A low-cost, enzyme-coupled fluorescent assay for rapid quantification of glycolysis rate of cells

Cai, Limeng; Wang, Yuqi; Yang, Ying; Wu, Hao

doi:10.1007/s00216-021-03834-2

Cited by 3 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chemical methods often use a cell-permeable peptide for intracellular protein delivery, which has enabled direct reprogramming using OCT-4 proteins, , cancer detection and treatment with lactate oxidase, , and analysis of protein dynamics in living cells . In contrast, physical methods such as electroporation, various nanowires (like silicon, diamond nanoneedles, gold, and ZnO), and hollow needle injection (including Si, Pt, carbon, and Al 2 O 3 ) transport target proteins through transient holes in the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

“…2 Consequently, methods that can directly deliver proteins into cells are sought after, although conventional methods face challenges like uniformly delivering the necessary amount of protein for functional expression in the cell without causing toxicity. 3 Chemical methods often use a cell-permeable peptide for intracellular protein delivery, 4 which has enabled direct reprogramming using OCT-4 proteins, 5,6 cancer detection and treatment with lactate oxidase, 7,8 and analysis of protein dynamics in living cells. 9 In contrast, physical methods such as electroporation, 10 various nanowires (like silicon, 11 diamond nanoneedles, 12 gold, 13 and ZnO 14 ), and hollow needle injection (including Si, 15 Pt, 16 carbon, 17 and Al 2 O 3 18 ) transport target proteins through transient holes in the cell membrane.…”

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Hybrid Nanotube Stamp System in Intracellular Protein Delivery for Cancer Treatment and NMR Analytical Techniques

Zhang,

Liu,

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

In contrast to intracellular gene transfer, the direct delivery of expressed proteins is a significantly challenging yet essential technique for elucidating cellular functions, including protein complex structure, liquid–liquid phase separation, therapeutic applications, and reprogramming. In this study, we developed a hybrid nanotube (HyNT) stamp system that physically inserts the HyNTs into adhesive cells, enabling the injection of target molecules through HyNT ducts. This system demonstrates the capability to deliver multiple proteins, such as lactate oxidase (LOx) and ubiquitin (UQ), to approximately 1.8 × 107 adhesive cells with a delivery efficiency of 89.9% and a viability of 97.1%. The delivery of LOx enzyme into HeLa cancer cells induced cell death, while enzyme-delivered healthy cells remained viable. Furthermore, our stamp system can deliver an isotope-labeled UQ into adhesive cells for detection by nuclear magnetic resonance (NMR).

show abstract

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

A Hybrid Nanotube Stamp System in Intracellular Protein Delivery for Cancer Treatment and NMR Analytical Techniques

Zhang,

Liu,

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

show abstract

Lactate‐Oxidase‐Instructed Cancer Diagnosis and Therapy

et al. 2023

View full text Add to dashboard Cite

Lactate oxidase (LOx) has attracted extensive interest in cancer diagnosis and therapy in recent years owing to its specific catalysis on l‐lactate; its catalytic process consumes oxygen (O2) and generates a large amount of hydrogen peroxide (H2O2) and pyruvate. Given high levels of lactate in tumor tissues and its tight correlation with tumor growth, metastasis, and recurrence, LOx‐based biosensors including H2O2‐based, O2‐based, pH‐sensitive, and electrochemical have been designed for cancer diagnosis, and various LOx‐based cancer therapy strategies including lactate‐depletion‐based metabolic cancer therapy/immunotherapy, hypoxia‐activated chemotherapy, H2O2‐based chemodynamic therapy, and multimodal synergistic cancer therapy have also been developed. In this review, the lactate‐specific catalytic properties of LOx are introduced, and the recent advances on LOx‐instructed cancer diagnostic or therapeutic platforms and corresponding biological applications are summarized. Additionally, the challenges and potential of LOx‐based nanomedicines are highlighted.

show abstract

The colors of peroxygenase activity: Colorimetric high-throughput screening assays for directed evolution

Dolz,

Monterrey,

Beltrán-Nogal

et al. 2023

Methods in Enzymology

View full text Add to dashboard Cite

A low-cost, enzyme-coupled fluorescent assay for rapid quantification of glycolysis rate of cells

Cited by 3 publications

References 25 publications

A Hybrid Nanotube Stamp System in Intracellular Protein Delivery for Cancer Treatment and NMR Analytical Techniques

A Hybrid Nanotube Stamp System in Intracellular Protein Delivery for Cancer Treatment and NMR Analytical Techniques

Lactate‐Oxidase‐Instructed Cancer Diagnosis and Therapy

The colors of peroxygenase activity: Colorimetric high-throughput screening assays for directed evolution

Contact Info

Product

Resources

About