A low molecular weight fraction of polyethylenimine (PEI) displays increased transfection efficiency of DNA and siRNA in fresh or lyophilized complexes

Novel degradable oligoethylenimine acrylate ester-based pseudodendrimers for in vitro and in vivo gene transfer

“…Within the PEI class of polymers, a lower molecular weight has previously been found to provide better biocompatibility. 38,39 In conclusion, we demonstrated novel degradable cationic pseudodendrimers useful for gene transfer in vitro and in vivo. In our studies, we observed a specific structure-activity relationship according to their biophysical and biological properties, including cytotoxicity and gene transfer efficiency.…”

Silencing of ribonucleotide reductase subunit M1 potentiates the antitumor activity of gemcitabine in resistant cancer cells

“…Thirty-three microliters of PEI25K (5 mg/ml) in 150 mM NaCl was added to the siRNA solution, resulting in a nitrogen-to-phosphorus (N/P) ratio of 33. 23 After vortexing, the mixture was incubated at room temperature for 30 min prior to further use. The particle size and zeta potential of the complexes were measured in triplicates with at least 12 runs for each measurement at 25°C using a Malvern Zetasizer Nano ZS.…”

“…20,22 It was shown that polyethylenimine (PEI) could protect siRNA from enzymatic and nonenzymatic degradations and efficiently deliver them into target cells in culture. 23,24 Moreover, it was shown that PEI can also efficiently deliver siRNA complexed with it to tumors in mice after systemic administration. 25, 26 We therefore chose to employ PEI as a carrier for RRM1-specific siRNA in this study.…”

Mixing-sequence-dependent nucleic acid complexation and gene transfer efficiency by polyethylenimine

“…1A) generated a significantly higher cytotoxicity. 38,39 NIH 3T3 cells expressing GFP (NIH 3T3/GFP cells) were incubated with siRNA/PEI polyplexes at 0.6 μg siRNA mL −1 at an N/P ratio of 10 ( Fig. 6).…”