A Low-Power Sensing System of VEGF Concentration with Monolithic Electrodes and An All-Digital Sub-Sampling Delay-Locked Loop

Sun, Tsung-Wen; Cheng, Ren-Wei; Tsai, Tsong-Chang

doi:10.1109/biocas54905.2022.9948620

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…The monolithic interdigitated electrodes were used as the transducer; post-process etching and Au plating were also applied to the surfaces of the electrodes. Experimental results showed that the biosensor achieved a capacitive resolution of 28.3 fF and a sensing range of VEGF concentration from 1 to 1000 pg/mL [18]. While both studies [17,18] achieved highly sensitive detection of VEGF, complex fabrication procedures as well as an expensive setup were required to attain this level of sensitivity, and this may limit practical applications of such techniques.…”

Section: Introductionmentioning

confidence: 99%

“…Experimental results showed that the biosensor achieved a capacitive resolution of 28.3 fF and a sensing range of VEGF concentration from 1 to 1000 pg/mL [18]. While both studies [17,18] achieved highly sensitive detection of VEGF, complex fabrication procedures as well as an expensive setup were required to attain this level of sensitivity, and this may limit practical applications of such techniques. Hence, there is a need to produce a more cost-effective interdigitated sensor that is capable of detecting VEGF under less ideal operating conditions.…”

Section: Introductionmentioning

confidence: 99%

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VEGF Detection via Impedance Spectroscopy on Surface Functionalized Interdigitated Biosensor

Lin

Zida

Yang

et al. 2023

JFB

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Vascular endothelial growth factor (VEGF), a clinically important biomarker, often plays a key role in angiogenesis, would healing, tumor growth, lung development, and in retinal diseases. Hence, detecting and quantifying VEGF is deemed medically important in clinical diagnosis for many diseases. In this report, a simple yet highly cost-effective platform was proposed for VEGF protein detection using commercially available interdigitated sensors that are surface modified to present DNA optimally for VEGF capture. The dielectric characteristics between the fingers of the sensor were modulated by the negatively charged aptamer-VEGF capture, and the impedance was estimated using an impedance analyzer. Impedance-spectra tests were compared among pristine unmodified surfaces, functionalized monolayer surfaces, and aptamer-grafted surfaces in order to evaluate the efficacy of VEGF detection. From our results, the sensitivity experiments as conducted showed the ability of the interdigitated sensor to detect VEGF at a low concentration of 5 pM (200 pg/mL). The specificity of the functionalized sensor in detecting VEGF was further examined by comparing the impedance to platelet-derived growth factor, and the results confirm the specificity of the sensor. Finally, the Nyquist plot of impedance spectra was also presented to help data visualization and the overall performance of the device was found to be a highly suitable template for a smart biosensor for the detection of VEGF.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

VEGF Detection via Impedance Spectroscopy on Surface Functionalized Interdigitated Biosensor

Lin

Zida

Yang

et al. 2023

JFB

View full text Add to dashboard Cite

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VEGF Detection via Impedance Spectroscopy on Surface Functionalized Interdigitated Biosensor

Lin¹,

Zida²,

Yang³

et al. 2023

Preprint

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Vascular endothelial growth factor (VEGF) is a important biomarker with significant clinical importance. It plays a key role in angiogenesis, would healing, tumor growth, lung development, and in retinal diseases. Hence, detecting and quantifying VEGF is crucial in clinical diagnosis and prognosis of various diseases. In this work, a simple but highly cost-effective platform is developed for VEGF protein detection by using commercially available interdigitated sensors that are modified through surface chemistry tagged with DNA aptamer grafting. The dielectric characteristics between the fingers of sensor is modulated by the negatively charged aptamer-VEGF capture and the impedance is estimated using impedance analyzer. Impedance spectra tests was compared between pristine unmodified, functionalized monolayer surfaces and aptamer-grafted surfaces in order to evaluate the efficacy of VEGF detection. In our results, the sensitivity experiments as conducted had shown the ability of the interdigitated sensor to detect VEGF at a low concentration of 5 pM. The specificity of the functionalized sensor in detecting VEGF is further studied by comparing the impedance to platelet-derived growth factor (PDGF), and the results confirm the specificity of the sensor. Finally, an alternative illustration of impedance spectra is also proposed to improve the data visualization, and such presentation may be potential for intelligent VEGF detection.

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A Low-Power Sensing System of VEGF Concentration with Monolithic Electrodes and An All-Digital Sub-Sampling Delay-Locked Loop

Cited by 2 publications

References 12 publications

VEGF Detection via Impedance Spectroscopy on Surface Functionalized Interdigitated Biosensor

VEGF Detection via Impedance Spectroscopy on Surface Functionalized Interdigitated Biosensor

VEGF Detection via Impedance Spectroscopy on Surface Functionalized Interdigitated Biosensor

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