A Lycium chinense-derived P5CS-like gene is regulated by water deficit-induced endogenous abscisic acid and overexpression of this gene enhances tolerance to water deficit stress in Arabidopsis

Guan, Chunfeng; Ji, Jing; Guan, Wenzhu; Feng, Yiyu; Li, Xiaozhou; Chen, Jin; Li, Jing; Wang, Yurong; Wang, Gang

doi:10.1007/s11032-014-0103-6

Cited by 16 publications

(10 citation statements)

References 56 publications

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“…The primers were all designed to produce fragments of ∼180–200 bp. QPCR reactions were carried out according to previous study . The constitutively expressed actin gene were used as an internal control to show the normalization of the amount of templates in the PCR reaction; the relative changes in target gene transcripts were calculated as X‐fold changes relative to the control or a selective sample.…”

Section: Methodsmentioning

confidence: 99%

Ectopic expression of EuWRI1, encoding a transcription factor in E. ulmoides, changes the seeds oil content in transgenic tobacco

Wang

Feng

et al. 2018

Biotechnology Progress

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The WRINKLED1 (WRI1) gene is a well-established key transcriptional regulator involved in the regulation of fatty acid biosynthesis in developing seeds. In this study, a new WRI1 gene was isolated from seeds of Eucommia ulmoides and named EuWRI1. A close link between gibberellins signaling and EuWRI1 gene expression was suggested in this study. Functional characterization of EuWRI1 was elucidated through seed-specific expression in tobacco. In transgenic tobacco, the expression of EuWRI1 in eight independent transgenic lines was detected by semiquantitative RT-PCR. The relative mRNA accumulation of genes encoding enzymes involved in fatty acid biosynthesis (biotin carboxyl carrier protein and keto-ACP synthase 1) was also assayed in tobacco seeds. Analysis of the seeds oil content and starch content indicated that the transgenic lines showed a significant increase in seeds oil content, whereas starch content decreased significantly. Further analysis of the fatty acid composition revealed that palmitic acid (16:0), linoleic acid (18:2) and linolenic acid (18:3) increased significantly in seeds of transgenic tobacco lines, but stearic acid (18:0) levels significantly declined. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:337-346, 2018.

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Section: Methodsmentioning

confidence: 99%

Ectopic expression of EuWRI1, encoding a transcription factor in E. ulmoides, changes the seeds oil content in transgenic tobacco

Wang

Feng

et al. 2018

Biotechnology Progress

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“…L. chinense plants were grown at the conditions as indicated in our previous study . Expression analysis of LcBiP gene in different tissues was carried out using 12 weeks old plants with 28 to 35 leaves.…”

Section: Methodsmentioning

confidence: 99%

“…For RT‐PCR analysis, total RNA isolated was used as the template for first‐strand cDNA synthesis using the TransScriptII One‐Step gDNA Removal and cDNA Synthesis SuperMix with random primers (TransGen Biotech). All protocols were performed according to our previous study . The primers used for RT‐PCR analysis were shown in Table .…”

Section: Methodsmentioning

confidence: 99%

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LcBiP, a endoplasmic reticulum chaperone binding protein gene from Lycium chinense, confers cadmium tolerance in transgenic tobacco

Guan

Chen

et al. 2015

Biotechnology Progress

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Cadmium (Cd) accumulation is very toxic to plants. The presence of Cd may lead to excessive production of reactive oxygen species (ROS), and then cause inhibition of plant growth. The endoplasmic reticulum chaperone binding protein (BiP) is an important functional protein, which has been shown to function as a sensor of alterations in the ER environment. BiP overexpression in plants was shown to increase drought tolerance through inhibition of ROS accumulation. Due to the above relationships, it is likely that there may be a link between Cd stress tolerance, ROS accumulation and the BiP transcript expression in plants. In this study, a BiP gene, LcBiP, from L. chinense was isolated and characterized. Overexpression of LcBiP in tobacco conferred Cd tolerance. Under Cd stress conditions, the transgenic tobacco lines exhibited better chlorophyll retention, less accumulation of ROS, longer root length, more glutathione (GSH) content, and less antioxidant enzyme activity than the wild type. These data demonstrated that LcBiP act as a positive regulator in Cd stress tolerance. It is hypothesized that the improved Cd tolerance of the transgenic tobacco plants may be due to the enhanced ROS scavenging capacity. The enhancement of GSH content might contribute to this ROS scavenging capacity in the transgenic plants. However, the underlying mechanism for BiP-mediated increase in Cd stress tolerance need to be further clarified.

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“…The primers were all designed to yield fragments of approximately 180-200 bp. QPCR reactions were carried out according to our previous study (Guan et al 2014a). The constitutively expressed ubiquitin gene were used as an internal control to show the normalization of the amount of templates in the PCR reaction; the relative changes in target gene transcripts were calculated as X-fold changes relative to the control samples.…”

Section: Stress Treatment On Transgenic Tobacco Overexpressing Lcherfmentioning

confidence: 99%

The glutathione synthesis may be regulated by cadmium-induced endogenous ethylene in Lycium chinense, and overexpression of an ethylene responsive transcription factor gene enhances tolerance to cadmium stress in tobacco

Guan

et al. 2015

Mol Breeding

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Glutathione (GSH) plays a pivotal role in heavy metal detoxification. Ethylene is one of the important plant hormones, which plays a critical role in triggering plant responses to different stresses such as cadmium (Cd) stress. Ethylene responsive transcription factor (ERF) belongs to one of the largest plant transcription factor families. ERF is known to be induced by ethylene and thus regulate multiple stress responses through the activation of stressrelated genes. Until now, little has been done to explore the relationship among the accumulation of endogenous ethylene, ERF transcript levels and the GSH content in plants under Cd treatment and we will investigate these link. In this study, the gene transcript level of LchERF, LchGSH1 (gene responsible for the first-step GSH biosynthesis) and LchGSHS (gene responsible for the second-step GSH biosynthesis), endogenous ethylene accumulation, GSH content and Cd concentration in Lycium chinense with or without Cd stress treatment were studied. Furthermore, the transgenic tobacco expressing 35S::LchERF which grown under Cd stress condition was also investigated in this study. Our results showed that endogenous ethylene, LchERF, LchGSH1 and LchGSHS gene expression and GSH content can be induced by Cd treatment in L. chinense, however, reduced by co-treatment with 2-aminoethoxyvinlglycine (AVG), an inhibitor of ethylene biosynthesis. The transgenic tobacco expressing 35S::LchERF showed greater tolerance to Cd stress than non-transgenic plants. The expression of NtGSH1 and NtGSHS genes was increased in transgenic tobacco plants compared with non-transgenic plants, indicating that LchERF is associated with the expression level of GSH synthesis related genes in tobacco. Evidence was presented here that under Cd stress, GSH accumulation occurred at least partially via enhanced LchERF gene expression and the ethylene signal transduction pathways might be involved in this accumulation.

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A Lycium chinense-derived P5CS-like gene is regulated by water deficit-induced endogenous abscisic acid and overexpression of this gene enhances tolerance to water deficit stress in Arabidopsis

Cited by 16 publications

References 56 publications

Ectopic expression of EuWRI1, encoding a transcription factor in E. ulmoides, changes the seeds oil content in transgenic tobacco

Ectopic expression of EuWRI1, encoding a transcription factor in E. ulmoides, changes the seeds oil content in transgenic tobacco

LcBiP, a endoplasmic reticulum chaperone binding protein gene from Lycium chinense, confers cadmium tolerance in transgenic tobacco

The glutathione synthesis may be regulated by cadmium-induced endogenous ethylene in Lycium chinense, and overexpression of an ethylene responsive transcription factor gene enhances tolerance to cadmium stress in tobacco

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