A Lysosome‐Activated Tetrahedral Nanobox for Encapsulated siRNA Delivery

Abstract

Tetrahedral framework nucleic acids (tFNAs) have attracted extensive attention as drug nanocarriers because of their excellent cellular uptake. However, for oligonucleotide cargos, tFNA mainly acts as a static delivery platform generated via sticky‐ended ligation. Here, inspired by the original stable space inside the tetrahedral scaffold, a dynamic lysosome‐activated tFNA nanobox is fabricated for completely encapsulating a short interfering RNA (siRNA) of interest. The closed tetrahedral structure endows cargo siRNA with greater resistance against RNase and serum and enables solid integration with the vehicle during delivery. Moreover, the pH‐responsive switch of nanobox allows the controlled release of siRNA upon entry into lysosomes at cell culture temperature. Based on protective loading and active unloading, an excellent silencing effect on the target tumor necrosis factor alpha (TNFα) gene is achieved in in vitro and in vivo experiments. Conclusively, the nanobox offers a dynamic pH‐sensitive confinement delivery system for siRNA and can be an extendable strategy for other small RNA.

Translocation of Specific DNA Nanocarrier through an Ultrasmall Nanopipette: Toward Single-Protein-Molecule Detection with Superior Signal-to-Noise Ratio

“…The current–voltage recording of 10 glass nanopores with a size of 30 nm demonstrated that their average rectification ratio of −1.0 V/+1.0 V was 2.7 ± 0.2, as is plotted in Figure S1, indicating the repeatable fabrication of identical nanopipettes. On the other hand, TDNs are programmable DNA nanostructures featuring good rigidity and stability and have been applied in increasing arenas. − Since altering the base pairs of the side chain of TDN had no obvious effect on the formation and stability of its structure, , herein we incorporated one AChE aptamer into one side chain of the TDN, empowering the as-designed TDN-apt with the capacity of AChE recognition. According to the sequences of five used strands as shown in Figure S2 and Table S1, the edge of the TDN was calculated to be ca.…”

Self-Assembled Immunostimulatory Tetrahedral Framework Nucleic Acid Vehicles for Tumor Chemo-immunotherapy

“…The immunostimulatory CpG was prolonged on the single-stranded DNA (ssDNA) and CpG-tFNA was fabricated according to the previous study. − Concisely, four designed ss CpG-DNAs (Sangon, Shanghai, China) were dissolved in TM buffer and denatured at 95 °C for 5 min. The detailed sequence of each ss CpG-DNA is displayed in Table .…”

Application of aptamers in regenerative medicine

“…In recent years, people have developed ways of using and treating NOX-D20. Li et al (2019) combined a framework of nucleic acid (FNA) ( Zhang et al, 2021b ; Zhang et al, 2021c ; Gao et al, 2022 ) in the form of a DNA origami nanostructure to improve the stability and targeting of NOX-D20 in vivo . They used bipyramidal FNA as a drug delivery platform to deliver NOX-D20 to treat cerebral ischemia-reperfusion injury and achieved good results.…”