A lysosome-targeting dual-functional fluorescent probe for imaging intracellular viscosity and beta-amyloid

Tan, Hui-ya; Qiu, Yutai; Sun, Han; Yan, Jin-wu; Zhang, Lei

doi:10.1039/c9cc00113a

Cited by 64 publications

(27 citation statements)

References 27 publications

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“…Zhang et al. synthesized lysosome‐targetable fluorescent probe 71 for detection of viscosity and beta‐amyloid [100] . Probe 71 was fabricated by combination of merocyanine dye with a 3‐morpholinopropylamine moiety (Figure 33).…”

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

117

115

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Viscosity, as a vital microenvironment parameter, is tightly associated with multitudinous cellular processes and diseases. Recently, precise visualization of viscosity has started to arouse more and more interest. However, owing to the complicated character, it is still a huge challenge to directly observe viscosity in living systems. In this regard, mounting fluorescence probes are being increasingly fabricated to map viscosity inside live cells and small animals. In this minireview, the viscosity‐sensitive small molecular fluorescent probes used in bioimaging are comprehensively summarized, mainly focusing on the last three years. Moreover, the current challenges and opportunities for the development of viscosity‐specific fluorescent probes will be discussed.

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Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

117

115

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“…Probe 125 was also applied to detect the lysosomal viscosity in SH-SY5Y cells and Aβ plaques in transfected PC12 (PC12-EGFP-Aβ) cells. [164] A dual-responsive fluorescent probe, probe 126 (Mito-VS, Figure 17) was constructed by Li et al to monitor the levels of mitochondrial viscosity and H 2 S. Due to the free intramolecular rotation between the pyridine and dimethylaniline groups, probe 126 per se was nonfluorescent. In high viscous solutions of 1.03-584.52 cP, due to restricted rotation, probe 126 showed strong red fluorescence with a maximum em at 610 nm and a fluorescence quantum yield of 0.62.…”

Section: Viscositymentioning

confidence: 99%

“…Probe 125 was also applied to detect the lysosomal viscosity in SH‐SY5Y cells and Aβ plaques in transfected PC12 (PC12‐EGFP‐Aβ) cells. [ 164 ]…”

Section: Alzheimer's Diseasementioning

confidence: 99%

Fluorescent Diagnostic Probes in Neurodegenerative Diseases

et al. 2020

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Both chronic and acute neurodegenerative diseases have been associated with high morbidity and mortality, along with the death of neurons in different areas of the brain, and there are few or no effective curative therapy options for treatment of patients. [3,4] These disorders are a major cause of concern for the health and quality of life in the aging society, since age is the greatest risk factor for neurodegenerative disease. [5] The World Health Organization has predicted that in next 20 years neurodegenerative disease will become the second most common cause of mortality after cardiovascular disease. [6] Neurodegenerative processes begin long before their clinical symptoms are evident, and evolve for years slowly and irreversibly. Hence, there is an urgent need to diagnose neurodegenerative disease as early as possible and to distinguish between different neurodegenerative disorders with shared and unique symptoms to facilitate decision making regarding choice of treatment. Timely diagnosis is important for the adoption of therapeutic modalities in clinical trials prior to moderate dementia exhibition, to appraise and apply antidotes to maximally preserve the cognitive functions or to slow down the course of these disorders. The ability to analyze and identify risk factors for cognitive deficit would represent an extraordinary advancement in the field of dementia. [5] The main modern diagnostic technologies for the early examination of neurodegenerative disease pathology, neuroimaging techniques, include radioligandsbased positron emission tomography (PET), 3D single-photon emission-computed tomography (SPECT), and structural magnetic resonance imaging (MRI). Although PET and SPECT are the most used imaging techniques for neurodegenerative diseases, their application is limited by high cost, involvement of hazardous radiolabeled compounds, need for sophisticated instruments, and application of complex data acquisition and analysis protocols. The MRI technique has relatively low spatial resolution as well as intrinsically poor sensitivity to distinguish morphological differences between disease biomarkers and the surrounding tissue. Recently, fluorescence diagnosis technology has become an attractive and potential alternative to diagnose and probe the progression of neurodegenerative diseases, because of being rapid, noninvasive, sensitive, simple, real-time, low-cost, and high-resolution in nature. [7] There are several uses for Neurodegenerative diseases are debilitating disorders that feature progressive and selective loss of function or structure of anatomically or physiologically associated neuronal systems. Both chronic and acute neurodegenerative diseases are associated with high morbidity and mortality along with the death of neurons in different areas of the brain; moreover, there are few or no effective curative therapy options for treating these disorders. There is an urgent need to diagnose neurodegenerative disease as early as possible, and to distinguish between different disorders with overlap...

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“…It is an acidic compartment and contains approximately 60 hydrolytic enzymes for breaking all sorts of biomolecules ( Lawrence and Zoncu, 2019 ). The viscosity of lysosome and lysosome-related organelles (LROs) is closely associated with overall animal health and a key indicator of its functionality ( Li et al, 2018 ; Tan et al, 2019 ; Cai et al, 2021 ). Therefore, sensitive monitoring of their viscosity with a specific nano-sized molecular viscometer is essential for understanding cellular health.…”

Section: Introductionmentioning

confidence: 99%

A Fluorogenic Far Red-Emitting Molecular Viscometer for Ascertaining Lysosomal Stress in Live Cells and Caenorhabditis elegans

2022

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The cellular physiochemical properties such as polarity, viscosity, and pH play a critical role in cellular homeostasis. The dynamic change of lysosomal viscosity in live cells associated with different environmental stress remains enigmatic and needs to be explored. We have developed a new class of Julolidine-based molecular viscometers with an extended π-conjugation to probe the lysosomal viscosity in live cells. High biocompatibility, pH tolerance, and the fluorogenic response with far red-emission (>600 nm) properties make these molecular viscometers suitable for live-cell fluorescence imaging in Caenorhabditis elegans. Among these probes, JIND-Mor is specifically designed to target lysosomes via simple modification. The real-time monitoring of lysosomal viscosity change under cellular stress was achieved. We believe that such a class of molecule viscometers has the potential to monitor lysosomal health in pathogenic conditions.

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A lysosome-targeting dual-functional fluorescent probe for imaging intracellular viscosity and beta-amyloid

Abstract: A lysosome-targeting dual-functional fluorescent probe was rationally designed and developed for imaging intracellular lysosomal viscosity and beta-amyloid.

Cited by 64 publications

References 27 publications

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Fluorescent Diagnostic Probes in Neurodegenerative Diseases

A Fluorogenic Far Red-Emitting Molecular Viscometer for Ascertaining Lysosomal Stress in Live Cells and Caenorhabditis elegans

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