A magnetic resonance study of the effect of nutritional status on cold-preserved murine liver

Dimicoli, Jean‐Luc; Patry, Jacqueline; Adam, René; Dennison, Ashley R.; Nédeléc, Jean-François; Bismuth, H

doi:10.1016/0016-5085(94)90819-2

Cited by 8 publications

(2 citation statements)

References 26 publications

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“…Furthermore, using 31 P-and 13 C-NMR, we have previously studied the recovery of isolated perfused livers from mice that had been fasted for 24 h or fed ad libitum prior to harvesting and cold preservation. 6,7 Under our conditions of observation, i.e. mice submitted to an 8 p.m. to 8 a.m. light-off period and an 8 a.m. to 8 p.m. light-on period, in livers excised after 1 p.m. and perfused at 37°C for 30 min before cold ischemia, the glycogen content was always low at the beginning of the cold storage.…”

Section: Introductionmentioning

confidence: 98%

The effect of dichloroacetate and alanine on the metabolic recovery of perfused mouse liver after cold ischemia

1999

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Section: Introductionmentioning

confidence: 98%

The effect of dichloroacetate and alanine on the metabolic recovery of perfused mouse liver after cold ischemia

1999

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“…The role of fructose-2,6-bisphosphate (FBP) in regulation of glucose fluxes was assessed using in vivo 13 C MRS in a mouse with elevated levels of FBP, showing that elevated FBP levels result in increased hepatic glycogen storage through indirect synthesis of glycogen [125]. The liver is readily amenable to perfusion ex vivo and studies have been performed on isolated murine livers to determine metabolism of exogenously administered compounds in the perfusion medium using 13 C MRS to determine best methods of liver preservation for transplantation [126]. Currently, relatively few studies have used such methodology to investigate differences in metabolic fluxes between transgenic animals.…”

Section: (C) Brainmentioning

confidence: 99%

The Application of In Vivo MRI and MRS in Phenomic Studies of Murine Models of Disease

So¹,

Bell²

Modern Magnetic Resonance

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Beneficial Effect of Alanine on Metabolic Recovery of Fasted Livers Submitted to Cold Ischemia

et al. 1996

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The aim of this study was to investigate the possible beneficial effect on perfused mouse liver of alanine as an exogenous substrate for gluconeogenesis. Livers from fed and fasted animals were perfused with oxygenated Krebs' Henseleit buffer for 30 min, then stored at 4°C in University of Wisconsin solution for 48 h. Then reperfusion at 37°C was performed according to two protocols. In the first one, reperfusion with alanine‐free Krebs' Henseleit buffer was used for 1 h. 8 mM (3‐13C) alanine was then added and perfusion was prolonged for a second hour. In the second one, the first hour of perfusion was omitted and the organs were reperfused directly for an hour in the presence of 8 mM (3‐13C)alanine. 31P NMR was used to measure the NTP recovery of the livers. At the end of the reperfusions, 13C and 1H NMR spectra of perfusates and of glutamine extracted from these perfusates by HPLC were recorded. These data were analysed according to a model of liver metabolism assuming that the only substrate of the liver was (3‐13C)alanine and endogenous substrates were metabolizable only through pyruvate. It was found that in the absence of initial alanine at reperfusion, livers from fasted mice recovered less NTP than those of fed ones (40±4% vs 60±5%, p<0.01), but not if this substrate is present at the beginning of reperfusion (61±5% vs 60±5%). This was confirmed by the amount of labelled metabolites produced. However, the dilution of 13C labelled metabolites by unlabelled ones did not indicate a larger concentration of endogenous substrates in livers from fed mice. The conclusion reached was that the lower pyruvate dehydrogenase activity of livers from fasted mice relatively to that from fed mice could be compensated for by the greater pyruvate concentration provided by alanine for the initial production of NTP after cold ischemia and warm reperfusion.

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A magnetic resonance study of the effect of nutritional status on cold-preserved murine liver

Cited by 8 publications

References 26 publications

The effect of dichloroacetate and alanine on the metabolic recovery of perfused mouse liver after cold ischemia

The effect of dichloroacetate and alanine on the metabolic recovery of perfused mouse liver after cold ischemia

The Application of In Vivo MRI and MRS in Phenomic Studies of Murine Models of Disease

Beneficial Effect of Alanine on Metabolic Recovery of Fasted Livers Submitted to Cold Ischemia

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