A maize defective-kernel mutant (longcell) characterized by tubular cells, severe morphological alterations and induction of cell death

Bastida, Miriam; Graziano, Enrique; Roca, Ramón; López, Inmaculada; Sánchez-Pons, Núria; Puigdomènech, Pere; Vicient, Carlos M.

doi:10.1007/s00425-005-0136-5

Cited by 7 publications

(12 citation statements)

References 54 publications

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“…In longcell mutant embryo development is arrested in the early stages of embryogenesis (Graziano et al, 2003;Bastida et al, 2006). We have identified and verified the differential expression of twelve genes.…”

Section: Gene Discovery In Maize Embryogenesismentioning

confidence: 63%

“…In situ hybridization analyses allow to identify morphologically aberrant tissues or to establish hierarchical links between genes (Fontanet and Vicient, 2008). Marker genes have been used in the study of plant embryo development in different plant species, including maize (Bastida et al, 2006).…”

Section: Gene Discovery In Maize Embryogenesismentioning

confidence: 99%

“…This enzyme catalyzes the conversion of glucose-6-phosphate to myoinositol 1-phosphate, which is the first product in the biosynthetic pathway of myo-inositol, which is, itself, the starting material for phytic acid synthesis. Other genes identified by differential display assay include genes encoding knotted, CDC48, an RNA binding protein, scarecrow, a protein kinase, a protease, cytochrome b245, β-chain NADpH oxidase, pectate lyase-like protein, histone deacetylase 2b and cyclophilin (Bastida et al, 2006).…”

Section: Gene Discovery In Maize Embryogenesismentioning

confidence: 99%

“…The mutant embryos are variable in shape but development is always blocked before the early coleoptilar stage. Although the endosperm is reduced in size, it has the typical vitreous texture, basal endosperm, transfer and aleurone layers (Bastida et al, 2006). Despite their abnormal shape, mutant embryos have often a suspensor-, scutellum-, embryo axis-and radicle-like structures, but they never develop the shoot apical meristem (SAM).…”

Section: B Amentioning

confidence: 99%

“…The expression of several marker genes was examined in the longcell mutants. During normal development, γ-zein mRNA accumulates in the subaleurone cells in the endosperm, but in longcell mutant γ-zein mRNA is accumulated in all endosperm cells (Bastida et al, 2006). The patterns of expression of genes involved in basic metabolic processes, such as glyceraldehyde phosphate dehydrogenase, acyl carrier protein (AP17) and a clathrin adapter protein, are not severely affected by the longcell mutation during early and mid embryogenesis.…”

Section: B Amentioning

confidence: 99%

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Genetic, molecular and cellular approaches to the analysis of maize embryo development

Josè-Estanyol

López-Ribera²,

Bastida³

et al. 2009

Int. J. Dev. Biol.

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The development of embryo structures in plants is essential for the formation of the adult plant organs. In cereals, this process has distinct features which have attracted attention from different points of view. Differential gene expression analyses have been used in order to identify genes useful as molecular markers of certain physiological, molecular or developmental processes. Several maize mutants affected in embryo development have been isolated, but only a fraction of them have been characterized at the molecular level. Molecular markers can be useful in the characterization of embryo defective mutants. Here, we describe the different techniques used in the identification of molecular marker genes for embryo development. We describe in more detail some groups of genes coding for cell wall proteins. We also describe the application of these molecular markers in the characterization of some embryo mutants.

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Section: Gene Discovery In Maize Embryogenesismentioning

confidence: 63%

Section: Gene Discovery In Maize Embryogenesismentioning

confidence: 99%

Section: Gene Discovery In Maize Embryogenesismentioning

confidence: 99%

Section: B Amentioning

confidence: 99%

Section: B Amentioning

confidence: 99%

See 3 more Smart Citations

Genetic, molecular and cellular approaches to the analysis of maize embryo development

Josè-Estanyol

López-Ribera²,

Bastida³

et al. 2009

Int. J. Dev. Biol.

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Post-acclimation transcriptome adjustment is a major factor in freezing tolerance of winter wheat

Skinner

2009

Funct Integr Genomics

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Cold-acclimated winter wheat plants were slowly frozen to -10 degrees C, and then the temperature was either maintained at -10 degrees C or was lowered further to -12 degrees C. Expression levels of a total of 423 genes were significantly altered in these treatments; genes upregulated outnumbered those downregulated by about a 9:1 ratio. Sixty-eight genes were upregulated at least fivefold in all freezing treatments; 17 of these 68 encoded transcription factors including C-repeat binding factor (Cbf), WRKY, or other Zn-finger proteins, indicating strong upregulation of genes involved in transcription regulation. Sixteen of the 68 highly upregulated genes encoded kinases, phosphatases, calcium trafficking-related proteins, or glycosyltransferases, indicating upregulation of genes involved in signal transduction. Six genes encoding chlorophyll a/b binding-like proteins were upregulated uniquely in response to the -12 degrees C treatment, suggesting a protective role of pigment-binding proteins in freezing stress response. Most genes responded similarly in the very freezing tolerant cultivar Norstar and in the moderately freezing tolerant Tiber, but some genes responded in opposite fashion in the two cultivars. These results showed that wheat crowns actively adapt as the temperature declines to potentially damaging levels, and genetic variation for this ability exists among cultivars.

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Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Garcia¹,

Almeida

Costa

et al. 2019

Plant Cell Tiss Organ Cult

110

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Somatic embryogenesis is a morphogenetic event where somatic cells have the ability to produce embryos without gamete fusion. It is used as a technique for plant mass propagation. It is a process that has six well defined steps such as induction, expression, development, maturation, germination and plant conversion. These steps are characterized by distinct physiological, morphological and molecular events. Although somatic embryogenesis has been established in several plant species, there remains many problems to be solved. The main problem in somatic embryogenesis is the large number of abnormal embryos produced which cannot germinate nor convert into normal plants. Abnormalities in somatic embryos (SE) can be generated by genetic or epigenetic changes in the DNA. These changes in the DNA can be influenced by external factors such as the use of plant growth regulators and mutagenic substances or stress factors applied to the plant tissue such as high and low temperatures, drought, salinity, and heavy metals. Abnormalities generated by genetic changes in the DNA are hardly reversible; however, abnormalities generated by epigenetic changes may be reversible and the abnormal embryos are able to produce normal plants in most cases. This review focuses on the identification of the main factors that can cause abnormal SE development in different plant species, suggest how SE abnormalities are related to somaclonal variations and identify which genes may be involved with embryo abnormalities. Zygotic embryo abnormalities in Arabidopsis thaliana mutants are listed with the aim to understand the main genetic mechanisms involved in embryo aberrations. Key messageThe abnormalities in somatic embryos are related to the use of 2,4-D in most of the published protocols, this sintetic auxin disrupts the endogenous auxin balance and the auxin polar transportation interfering with the embryo apical-basal polarity.

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A maize defective-kernel mutant (longcell) characterized by tubular cells, severe morphological alterations and induction of cell death

Cited by 7 publications

References 54 publications

Genetic, molecular and cellular approaches to the analysis of maize embryo development

Genetic, molecular and cellular approaches to the analysis of maize embryo development

Post-acclimation transcriptome adjustment is a major factor in freezing tolerance of winter wheat

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

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