A major-effect genetic locus, ApRVII, controlling resistance against both adapted and non-adapted aphid biotypes in pea

Ollivier, Rémi; Glory, Isabelle; Cloteau, Romuald; Gallic, Jean-François Le; Denis, Gaëtan; Morlière, Stéphanie; Miteul, Henri; Rivière, Jean-Philippe; Lesné, Angélique; Klein, Anthony; Aubert, Grégoire; Kreplak, Jonathan; Burstin, Judith; Pilet‐Nayel, Marie‐Laure; Simon, Jean‐Christophe; Sugio, Akiko

doi:10.1007/s00122-022-04050-x

Cited by 11 publications

(15 citation statements)

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“…Five genotypes of Pisum sativum (AeD99OSW- 49-5-7, NEVE, CAMEOR, CE101 = FP and CHEROKEE) and one genotype of Pisum fulvum (WT304) were studied. These genotypes showed contrasted differences in resistance levels against one or the other A. pisum biotypes in our previous work [ 13 ]. Details about these six Pisum genotypes are given in Table S1 .…”

Section: Methodsmentioning

confidence: 90%

“…As a first step of this study, we measured the body mass of individual aphids feeding on the different Pisum genotypes to confirm the resistance ranking obtained with a fecundity assay in an earlier study [ 13 ] ( Table S1 ). Body mass and fecundity scores are commonly used to screen for plant resistance as they best capture the impact of different plant resistance on aphid fitness [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

“…This confirms the overall better adaptation of the pea biotype ArPo28 on P. sativum [ 21 ]. It should be recalled here that the fecundity scores from the previous study [ 13 ] were obtained using a different protocol for ArPo28 and LSR1 (fecundity was measured from three first instar aphids for ArPo28 while it was measured from 10 mixed-age aphids for LSR1) so that direct comparison was not possible.…”

Section: Discussionmentioning

confidence: 99%

“…The two biotypes of A. pisum , a pea-adapted biotype, the ArPo28 clone, and an alfalfa-adapted biotype, the LSR1 clone, were provided by INRAE Le Rheu [ 13 ]. These aphid clones are deprived of any secondary symbionts reported in A. pisum .…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study, we conducted an analysis on the interaction between the 240 genotypes and A. pisum clones. Our fecundity assay using an A. pisum clone adapted to pea (pea biotype, ArPo28 clone) and alfalfa (alfalfa biotype, LSR1 clone) revealed a continuum in plant susceptibility and resistance [ 13 ]. None of the pea genotypes were totally resistant to the A. pisum pea biotype, while none of the pea genotypes were totally susceptible to the A. pisum alfalfa biotype.…”

Section: Introductionmentioning

confidence: 99%

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Aphid Resistance in Pisum Affects the Feeding Behavior of Pea-Adapted and Non-Pea-Adapted Biotypes of Acyrthosiphon pisum Differently

González

Simon

Sugio

et al. 2022

Insects

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Resistant genotypes of crops have emerged as an alternative and sustainable solution to pesticide use against pest insects. The resistance depends on the genetic diversity of the host plant and the pest species and can cause an alteration of the insect behavior. The aim of this work was to characterize the resistance level of different Pisum genotypes (one P. fulvum and five P. sativum genotypes) to two biotypes of the aphid Acyrthosiphon pisum, respectively adapted to pea and alfalfa, by measuring the individual aphid weight and analyzing aphid feeding behavior by electropenetrography (EPG). Aphid body mass was influenced by Pisum genotypes reflecting variation in their resistance level. P. fulvum was the most resistant to the A. pisum pea biotype (ArPo28 clone) and showed intermediate resistance to the A. pisum alfalfa biotype (LSR1 clone). The resistance levels of the five P. sativum genotypes to the two aphid biotypes were variable and more pronounced for the alfalfa biotype. EPG data showed that ArPo28 on P. fulvum and LSR1 on all the Pisum genotypes spent shorter time phloem feeding compared to ArPo28 on P. sativum genotypes, indicating that the resistance of Pisum genotypes to non-adapted A. pisum resides in mesophyll and phloem cells. In the meantime, ArPo28 on P. sativum genotypes with a different level of resistance spent a similar length of time phloem feeding, indicating that the quality of phloem sap of the resistance genotypes may not be optimal for the aphid. The study indicated that the resistance of Pisum genotypes to the two A. pisum biotypes involves different genetic factors and mechanisms that affect the aphid differently.

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Section: Methodsmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%