A male accessory gland peptide that regulates reproductive behavior of female D. melanogaster

Chen, P.S.; Stumm-Zollinger, Elisabeth; Aigaki, Toshiro; Balmer, J.; Bienz, Marianne; Bőhlen, Peter

doi:10.1016/0092-8674(88)90192-4

Cited by 623 publications

(524 citation statements)

References 27 publications

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“…1) [8,9]. The Hyp was shown to be (2S,4R)-4-hydroxyproline by comparison to a synthetic standard [9], and is consistent with the ability of all known prolyl-4-hydroxylases, including that from D. melanogaster, to incorporate the hydroxyl group in the R-configuration.…”

Section: Introductionsupporting

confidence: 68%

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NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster

2011

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a b s t r a c tThe insect sex peptide (SP) elicits a variety of biological responses upon transfer to the mated female. SP contains 36 amino acids, including a tryptophan-rich N-terminal region, a central region containing five hydroxyproline (Hyp) residues, and a C-terminal region enclosed by a disulfide bridge. The solution structure of SP, studied here using NMR spectroscopy, includes a motif WPWN that adopts a type I b-turn in the N-terminal Trp-rich region. This turn region is connected to the central Hyp-rich region, which adopts extended and/or PPII-like conformations. The C-terminal disulfide-bonded loop populates helical turns or nascent helical structure. Overall, the results reveal a rather flexible peptide that lacks a compact folded structure in solution.

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Section: Introductionsupporting

confidence: 68%

“…Information about the structure of SP in solution is of interest in understanding how this peptide hormone might Synthetic SP was assayed for oviposition and receptivity according to standard procedures [8], and was found to show full activity. :1), pH 5.0.…”

Section: Introductionmentioning

confidence: 99%

NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster

2011

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“…SP leads to rejection of courting males and increases egg production and oviposition [4][5][6][7][8], but also leads to other behavioural and physiological changes. These include an increase in feeding, a change in food choice and sleep, and stimulation of the immune system [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours

et al. 2013

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Male-derived sex-peptide (SP) induces profound changes in the behaviour of Drosophila females, resulting in decreased receptivity to further mating and increased egg laying. SP can mediate the switch in female reproductive behaviours via a G protein-coupled receptor, SPR, in neurons expressing fruitless, doublesex and pickpocket. Whether SPR is the sole receptor and whether SP induces the postmating switch in a single pathway has not, to our knowledge been tested. Here we report that the SP response can be induced in the absence of SPR when SP is ectopically expressed in neurons or when SP, transferred by mating, can access neurons through a leaky blood brain barrier. Membrane-tethered SP can induce oviposition via doublesex, but not fruitless and pickpocket neurons in SPR mutant females. Although pickpocket and doublesex neurons rely on G(o) signalling to reduce receptivity and induce oviposition, G(o) signalling in fruitless neurons is required only to induce oviposition, but not to reduce receptivity. Our results show that SP's action in reducing receptivity and inducing oviposition can be separated in fruitless and doublesex neurons. Hence, the SP-induced postmating switch incorporates shared, but also distinct circuitry of fruitless, doublesex and pickpocket neurons and additional receptors.

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“…When injected into (Chen et al, 1988) or ectopically expressed (Aigaki et al, 1991) in females, SP causes several profound changes in female behaviour. Two of the most striking of these are increased egg laying and reduced acceptance of male-mating attempts (Chapman et al, 2003;Liu and Kubli, 2003).…”

Section: Introductionmentioning

confidence: 99%

The consequences of genetic variation in sex peptide expression levels for egg laying and retention in females

et al. 2012

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The accessory gland proteins (Acps) that male Drosophila melanogaster produce and transfer to females during copulation are key to male and female fitness. One Acp, the sex peptide (SP), is largely responsible for a dramatic increase in female egg laying and decrease in female receptivity after copulation. While genetic variation in male SP expression levels correlate with refractory period duration in females, it is unknown whether male SP expression influences female egg laying or if any effect of SP is mediated by SP retention in the female reproductive tract. Here we measured the amount of SP retained in the female reproductive tract after mating and female egg laying after copulating with virgin males. We found no correlation between male SP expression levels and egg laying, or the amount of SP in the female reproductive tract after mating. Additionally, the amount of SP retained in the female did not influence egg laying. These finding suggests that additional factors, such as variation in other Acps, are important for the retention of SP in females and its quantitative effects on egg laying. It also shows that egg laying and refractory period response to SP is at least partially uncoupled.

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A male accessory gland peptide that regulates reproductive behavior of female D. melanogaster

Cited by 623 publications

References 27 publications

NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster

NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster

Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours

The consequences of genetic variation in sex peptide expression levels for egg laying and retention in females

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