A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.)

Urasaki, Naoya; Tokumoto, Masakazu; Tarora, Kazuhiko; Ban, Yasunori; Kayano, Toshiaki; Tanaka, Hiroshi; Oku, Hirosuke; Chinen, Isao; Terauchi, Ryohei

doi:10.1007/s001220100693

Cited by 112 publications

(86 citation statements)

References 11 publications

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“…Only a sex-linked DNA marker (e.g., AlstromRapaport et al 1998, Kafkas et al 2001, Urasaki et al 2002, not yet available for J. virginiana, File # 01TQ that could be used on seeds and seedlings would help to clarify if there are any reasons other than chance for the occasional local bias in sex ratio. Such a marker could also be used to determine the sex of non-reproductives, testing the hypothesis of the lack of reproduction by a greater proportion of the smaller female individuals under more limiting conditions.…”

Section: Results Growth Rates and Heightsmentioning

confidence: 99%

Growth Rates, Survivorship, and Sex Ratios of Juniperus virginiana on the New Jersey Piedmont from 1963 to 2000

Quinn¹,

Meiners²

2004

Journal of the Torrey Botanical Society

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Section: Results Growth Rates and Heightsmentioning

confidence: 99%

Growth Rates, Survivorship, and Sex Ratios of Juniperus virginiana on the New Jersey Piedmont from 1963 to 2000

Quinn¹,

Meiners²

2004

Journal of the Torrey Botanical Society

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“…Identification of sex-specific DNA markers would assist in alleviating this problem, if such linked markers are reliable and unaltered by the environment. Previous studies have provided preliminary data indicating that Random Amplified Polymorphic DNA (RAPD) markers might be useful for detecting sex expression in papaya [7][8][9][10][11][12] . Moreover, papaya male and hermaphrodite sex linked RAPD and SCAR markers have been identified by Deputy et al 13 and Urasaki et al 11,12 .…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have provided preliminary data indicating that Random Amplified Polymorphic DNA (RAPD) markers might be useful for detecting sex expression in papaya [7][8][9][10][11][12] . Moreover, papaya male and hermaphrodite sex linked RAPD and SCAR markers have been identified by Deputy et al 13 and Urasaki et al 11,12 . Genetic relationships among different C. papaya cultivars have been analyzed using amplified fragment length polymorphic (AFLP) markers 5 .…”

Section: Introductionmentioning

confidence: 99%

Detection of sequence characterized amplified region (SCAR) markers linked to sex expression in Carica papaya L.

Niroshini

Everard

Karunanayake

et al. 2008

J. Natn. Sci. Foundation Sri Lanka

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Carica papaya L. exhibits monoecious and dioecious plants that usually take six months for phenotypic manifestation. Nursery culling aided by sex-specific DNA markers was envisaged to alleviate the unnecessary cost incurred by farmers for maintaining unproductive male plants that contribute to 40-50% of the population. The mechanism of sex determination in papaya has been described as a tri-allelic single gene system with alleles, M 1 -dominant for maleness, M 2 -dominant for hermaphrodism and m-recessive for femaleness with diploid zygotes; M 1 M 1 , M 2 M 2 and M 1 M 2 being inviable. Bulked DNA samples of male, female and hermaphrodite plants were amplified by Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) using 100 random primers. Twenty of most promising of these were analyzed among individual sex types. Two sex-specific fragments, OPC09-1.7 and OPE03-0.4 were associated with maleness and hermaphrodism. The segregation of these two markers was analyzed in the F 1 population obtained by selfpollinating a hermaphrodite plant. A linkage was detected between the RAPD markers, OPC09-1.7 and OPE03-0.4 and the male and hermaphrodite sex of papaya plants. These sex-specific RAPD fragments were cloned and sequenced for converting them to more authentic SCAR markers. The Southern blot hybridization of RAPD-PCR products obtained by amplification of female, male and hermaphrodite papaya DNA amplified by OPC09 primer using radio labeled recombinant plasmid detected a polymorphic fragment in male and hermaphrodite papaya sex types. The nucleotide sequence of OPC09-1.7 fragment showed the possibility of developing more authentic SCAR markers to enhance the accurate sex determination of Carica papaya at the nursery stage.

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“…Estas técnicas permiten una identificación rápida y confiable, son cómo-das y algunas no requieren del conocimiento previo sobre la secuencia que se amplifica. Para diferenciar el sexo en papaya se han desarrollado marcadores moleculares tipo secuencias caracterizadas de regiones amplificadas (sequence characterized amplified región -SCAR-) (Deputy et al, 2002;Urasaki et al, 2002;Magdalita y Mercado, 2003;Chaves y Núñez, 2007). En Colombia, Chaves y Núñez (2007) desarrollaron el marcador SCAR-SDSP y en la Universidad de Hawai se desarrollaron los marcadores SCAR T1 y W11.…”

Section: R E S U M E Nunclassified

Evaluación de marcadores moleculares tipo SCAR para determinar sexo en plantas de papaya (Carica papaya L.)

Sánchez-Betancourt

Zarantes

2009

CTA

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En este estudio se emplearon los marcadores moleculares SCAR-SDSP, SCAR T1 y W11, registrados como secuencias específicas, para discriminar el sexo en las plántulas de papaya y validar su efectividad en plantas florecidas en campo. El SCAR-SDSP amplificó una banda de 369 pares de bases en plantas machos y hermafroditas, lo que permitió corroborar los resultados obtenidos con este marcador, desarrollado en Corpoica en un estudio anterior que utilizó genotipos colombianos. Con el uso de los marcadores SCAR T1 y W11 combinados en una sola reacción, se diferenció el sexo de plantas hembras y hermafroditas. En la predicción de sexos, en una muestra ciega utilizando plántulas de dos meses de edad derivadas de semilla comercial, se observó una proporción de 1:1 de hembras:hermafroditas, lo esperado según el patrón de segregación en un cruce entre estos dos tipos de plantas. La amplificación con el marcador SCAR W11 generó una banda de 800 pares de bases en plantas machos y hermafroditas, independientemente del lugar de procedencia de la muestra. Sin embargo, cuando esta banda se secuenció y analizó con el programa BLAST mostró identidad de 98% entre las secuencias de machos y hermafroditas. Evaluation of SCAR molecular markers to determine sex in papaya plants (Carica papaya L.) In this study the molecular markers SCARSDSP, SCAR T1 and W11 -reported as sex discriminators at seedling level-, were used to validate the effectiveness in flowered plants in the field. SCAR-SDSP amplified a band of 369 bp in male and hermaphrodite plants which allows corroborate the results obtained with this marker, developed at Corpoica using Colombian genotypes, in a previous study. With the use of the SCAR T1 and W11 markers combined in one reaction, it was possible to differentiate female and hermaphrodite plants. When the sex prediction was done with a blind sample using two months-old plantlets, from commercial seeds, a ratio of 1:1 female:hermaphrodite was observed, according to the segregation pattern of a cross between these two types of plants. The amplification with the SCAR W11 generated a band of 800 bp in male and hermaphrodite plants, independently of the sample’s place of origin. However, when this band was sequenced and analyzed with the BLAST program, the analysis showed 98% identity between male and hermaphrodite sequence.

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A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.)

Cited by 112 publications

References 11 publications

Growth Rates, Survivorship, and Sex Ratios of Juniperus virginiana on the New Jersey Piedmont from 1963 to 2000

Growth Rates, Survivorship, and Sex Ratios of Juniperus virginiana on the New Jersey Piedmont from 1963 to 2000

Detection of sequence characterized amplified region (SCAR) markers linked to sex expression in <i>Carica papaya</i> L.

Evaluación de marcadores moleculares tipo SCAR para determinar sexo en plantas de papaya (Carica papaya L.)

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