A mannose binding protein is involved in the adherence of species to inert surfaces

Abstract

Some carbohydrates are known to decrease the attachment of Acanthamoeba sp. to biological surfaces. By a method based on the reduction of a tetrazolium salt (XTT) by the mitochondrial dehydrogenases of the parasites, d-mannose and alpha-d-mannopyranoside have been shown to reduce Acanthamoeba attachment to inert surfaces, indicating that the mannose binding protein of Acanthamoeba trophozoites is involved in adherence to inert surfaces. The reduction in attachment is dose dependant and is not linked with a potential toxicity of the carbohydrates. All the species of Acanthamoeba tested were concerned by this mannose binding protein, but the adhesion of A. culbertsoni was also reduced by the presence of glucose.

Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression

“…Previous studies proved that the mannose-binding proteins and laminin-binding proteins on Acanthamoeba are the virulence proteins responsible for the pathogenesis of Acanthamoeba infection [ 26 , 31 , 35 , 59 ]. Pre-treated amoeba with the mannose showed a significant decrease in their adhesion and invasion on the collagen matrix and reduced their cytopathic effect [ 32 , 36 ].…”

“…Pre-treated amoeba with the mannose showed a significant decrease in their adhesion and invasion on the collagen matrix and reduced their cytopathic effect [ 32 , 36 ]. Incubation amoeba with mannose sugars inhibited their attachment on inert surfaces in a dose-dependent way [ 59 , 60 ]. Acanthamoeba trophozoites pretreated with anti-MBP IgY antibody significantly inhibited the CPE.…”

TLR4: the receptor bridging Acanthamoeba challenge and intracellular inflammatory responses in human corneal cell lines

“…Binding is the first key step in the pathogenesis of the infection 20 . A mannose‐binding protein, which is expressed on the surface of the parasites, has an important role in parasite–host interactions 21 , 22 . To date, the molecule(s) that parasites or mannose‐binding protein bind remain elusive.…”

Searching for the sweet spot of amoebic gill disease of farmed Atlantic salmon: the potential role of glycan-lectin interactions in the adhesion of Neoparamoeba perurans

“…For example, the ability of the amoeba Dictyostelium discoideum to bind to diverse hydrophobic and hydrophilic surfaces (including BSA-treated glass) was suggested to occur via van der Waals attraction between glycoproteins on the surface of the cell and the underlying substratum (Loomis et al, 2012). Different surface membrane molecules were also proposed by Imbert-Bouyer et al (2004) to be involved in the capacity of Acanthamoeba sp. to bind to inert surfaces such as plastic.…”