A Manual of Morphometry in Diagnostic Pathology

Baak, J. P. A.; Oort, J.

doi:10.1007/978-3-642-74823-3

Cited by 156 publications

(56 citation statements)

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“…Mitotic figures were recognized according to the criteria described by Baak and Oort (1983). In addition the mitotic activity index (MAI) was calculated and expressed as the number of mitoses per 1000 cells.…”

Section: Mitotic Counting In Breast Cancersmentioning

confidence: 99%

Immunodetectable cyclin D1is associated with oestrogen receptor but not Ki67 in normal, cancerous and precancerous breast lesions

et al. 2001

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Cyclin D1 is associated with cell cycle regulation and has more recently been shown to stimulate the transcriptional functions of the oestrogen receptor (ER). Furthermore, in normal breast there is a negative association between expression of ER and the proliferation marker Ki67 indicating that either ER positive cells are non-dividing or that the receptor is down-regulated as cells enter cycle. This important relationship breaks down in many ER-positive cancers and precancerous breast lesions where the receptor is often detected on proliferating cells. The aims of the present study were to determine the interplay between ER, Ki67 and cyclin D 1 in individual cells within the spectrum of human breast lesions ranging from normal to invasive carcinoma by using dual staining immunofluorescence. We found that in normal breast there was a strong positive association between ER and cyclin D 1 expression. In contrast there was a strong negative association between cyclin D 1 and Ki67 expression. Similar findings were seen for the other precancerous and cancerous breast lesions. Thus immunodetectable cyclin D 1 within individual cells does not appear to be associated with cell cycle progression in the benign or malignant breast but instead may have important interactions with ER. © 2001 Cancer Research Campaign http://www.bjcancer.com

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Section: Mitotic Counting In Breast Cancersmentioning

confidence: 99%

Immunodetectable cyclin D1is associated with oestrogen receptor but not Ki67 in normal, cancerous and precancerous breast lesions

et al. 2001

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“…Their number started to grow since as early as 1 st day of the experiment, exceeding control value by 4.9 times on average ( Table 2). Among other effects, surfactant system slows down the differentiation of dendritic cells thereby increasing the production of proinflammatory cytokines [17].…”

Section: Resultsmentioning

confidence: 99%

“…Morphometry study was carried out in a 100 point closed test system with an area of 3.52 × 10 4 μ 2 (to determine numerical densities (Nai) and volume densities (Vv)) and using AxioVision 4.12 software [17]. The means were calculated using Statistica 6 software.…”

Section: Methodsmentioning

confidence: 99%

Morphofunctional Characteristics of Pulmonary Surfactant System and Its Effect on Immune Cells in Influenza A (H1N1) Pathogenesis

Ковнер¹,

Потапова²,

Shkurupy³

et al. 2016

OJPathology

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“…For our investigations representative samples of the following diagnostic categories were selected: tubular adenomas (n = 8), papillary cystadenomas (n = 7), fibroadenomas (n = 10), noninvasive (n = 9) and invasive (n = 7) tubulopapillary carcinomas, noninvasive (n = 9) and invasive (n = 8) cribriform-comedo carcinomas and metastasising carcinomas (n = 5). (Baak et al, 1982(Baak et al, , 1983.…”

Section: Histological Examinationmentioning

confidence: 99%

Flow cytometric DNA measurement and cytomorphometric analysis of formalin fixed rat mammary tumours

Gijbels

Visser²,

Solleveld³

et al. 1991

Br J Cancer

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Summary Archival paraffin embedded material was used to examine whether additional quantitative criteria would be helpful to discriminate between histologically benign and malignant rat mammary tumours. To this end nuclear DNA content expressed as DNA ploidy index (DI) was measured using flow cytometry (FCM) (Van Zwieten, 1984 similarity between human and rat cellular characteristics can be established.In the present study, nuclear DNA content of spontaneous or radiation induced mammary tumours was measured by flow cytometry (FCM) using formalin fixed tissue samples. These data are compared with histological malignancy grade, proliferative activity as indicated by the frequency of mitoses and cytological characteristics such as nuclear area, perimeter and nuclear irregularity. Materials and methods AnimalsParaffin blocks from rat mammary tissues used for FCM were derived from radiation carcinogenesis studies described by Van Zwieten (1984). Radiation induced an increased incidence and a shortened latency period of mammary tumours while exhibiting the same spectrum of histological diagnoses. Estrogen treatment enhanced the effect of radiation. In these studies inbred female Wag/Rij rats and Sprague-Dawley rats in the 6th generation of inbreeding were obtained from the specified pathogen free stock colony. After bilateral or multilateral total body irradiation, as described earlier (Van Zwieten, 1984), at the age of 8 weeks, they were housed in experimental rooms under conventional conditions. Rats of certain experimental groups were administered an exogenous estrogenic hormone. The health of the experimental animals was followed closely by bacteriological and serological monitoring. The animals were kept for their entire lifespan, clinically examined weekly and a complete gross necropsy was performed on those found dead or killed moribund. In addition to sampling of mammary tissues a complete set of other tissues and gross lesions were collected at the same time for fixation in 10% phosphate buffered formalin, paraffin embedding and preparation of histological slides. For the present study we used representative mammary tumour samples of the major diagnostic categories (see later). We excluded animals which were found dead and tumour tissue samples with necrosis exceeding 10% of the tumour area. The great majority of the 63 mammary tumours used for this study was derived from the treatment groups i.e. 39 animals which were irradiated and estrogen treated, 20Br.

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A Manual of Morphometry in Diagnostic Pathology

Cited by 156 publications

References 0 publications

Immunodetectable cyclin D1is associated with oestrogen receptor but not Ki67 in normal, cancerous and precancerous breast lesions

Immunodetectable cyclin D1is associated with oestrogen receptor but not Ki67 in normal, cancerous and precancerous breast lesions

Morphofunctional Characteristics of Pulmonary Surfactant System and Its Effect on Immune Cells in Influenza A (H1N1) Pathogenesis

Flow cytometric DNA measurement and cytomorphometric analysis of formalin fixed rat mammary tumours

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