A maternal high fat diet has long‐lasting effects on skeletal muscle lipid and PLIN protein content in rat offspring at young adulthood (1162.7)

Abstract

High fat diets (HFD) can have adverse effects on skeletal muscle development. Skeletal muscle PLIN proteins (PLIN2, 3, &amp; 5) are thought to play critical roles in lipid metabolism, however effects of HFD on PLINs as well as lipases (HSL, ATGL, CGI‐58) have yet to be investigated. The objective of this study was to determine whether HFD would influence skeletal muscle lipase and PLIN protein content in dams as well as offspring at weaning (19d) and young adulthood (3mo). Female rats (28d old, n=9/group) were fed control (CON, AIN93G, 7% soybean oil) or HFD (AIN93G, 20% lard) for 10wks prior to mating, throughout pregnancy and lactation. Offspring were weaned onto CON (n=18/group, one female &amp; one male pup per litter was studied at 19d &amp; 3mo of age). Because there was no effect of sex for outcomes measured, male and female data was combined. HFD resulted in increased lipid content in plantaris of dams and pups, both at weaning and at 3mo (p=0.07). HFD resulted in increased PLIN3 content in plantaris of dams (P=0.016) and increased PLIN5 content in pups at weaning and 3mo (p=0.05). PLIN2 and PLIN5 content increased at 3mo vs. weaning (P<0.001). Diet had no effect on ATGL, CGI‐58, or HSL content. The data suggest that exposure to a maternal HFD results in increased skeletal muscle lipid and PLIN5 content in offspring through to young adulthood. Funded by NSERC.