A mechanism for fibrin monomer aggregation based on a kinetic study

Effect of cold storage on shear-induced platelet aggregation and clot strength

“…The heightened aggregation of 4-C-stored platelets under shear conditions may be attributable to two complementary mechanisms. 20,21,38,39 The rates of gelation between fresh and 4-C-stored samples were comparable, suggesting that the factors contributing to fibrin polymerization, namely, fibrinogen levels, plasma factors such as factor XIII or plasminogen activation inhibitors, and thrombin generation by platelets, are preserved during cold storage. 7,33,36 Second, because cold storage partially activates platelets, either as described earlier or via other mechanisms, and low temperature generally preserves the internal enzymatic machinery in cells, the platelets are thus ''primed'' for a maximal response to shear forces.…”

“…20,21 Clotting was initiated in fresh or stored PRP using 0.16-U human >-thrombin (Haematologic Technologies Inc., Essex Junction, VT) and 3-mM calcium chloride to overcome citrate inhibition and activate factor XIII, promoting fibrin cross-linking. 20,21 Clotting was initiated in fresh or stored PRP using 0.16-U human >-thrombin (Haematologic Technologies Inc., Essex Junction, VT) and 3-mM calcium chloride to overcome citrate inhibition and activate factor XIII, promoting fibrin cross-linking.…”

Determination of Soluble Fibrin by Turbidimetry of its Protamine Sulphate-Induced Paracoagulation

“…A pH value between 5.8 -7.0 leads to lateral polymerisation of soluble fibrin, which produces thick fibrils and therefore a photometrically well detectable turbidity (23,27). Only a few authors have used pH values outside this ränge (14,27).…”

“…Recent kinetic investigations of fibrin aggregation were^also done at short wavelengths (21)(22)(23). In coritrast, measurement at 610 nm s recommended by Lipinski (17) decreased the sensitivity.…”