A mechanism of antioxidant activity of calcium antagonist verapamil against LDL oxidation

Karmansky, Israel; Gruener, Nachman

doi:10.1016/s0009-8981(96)06472-8

Cited by 13 publications

(8 citation statements)

References 8 publications

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“…The quest for Cu-chelators for inhibiting oxidative damage resulted in the identification of compounds such as clioquinol [18] and verapamil [19]. The lack of biocompatibility of many of the synthetic metal chelators triggered the search for naturally occurring metal chelators.…”

Section: Introductionmentioning

confidence: 99%

Can nucleotides prevent Cu-induced oxidative damage?

Baruch-Suchodolsky

Fischer

2008

Journal of Inorganic Biochemistry

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Section: Introductionmentioning

confidence: 99%

Can nucleotides prevent Cu-induced oxidative damage?

Baruch-Suchodolsky

Fischer

2008

Journal of Inorganic Biochemistry

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“…But so far the experimental data on antioxidant properties have been strongest for CCB and in comparative studies CCB have had more pronounced effects than e.g. beta blockers (21)(22)(23)(24). Thus, our in vivo results are clearly in contrast with experi-623 mental studies.…”

Section: Discussionmentioning

confidence: 99%

Neutrophil free oxygen radical production and blood total antioxidant capacity in patients with coronary heart disease using various medications^Note

Mattila

Ristola

Repo

et al. 2001

APMIS

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Despite convincing results of studies in vitro, less is known about the effects of antioxidants on in vivo redox balance in humans. We developed a novel parameter of in vivo redox balance, and studied it and its relation to dental infections in 51 patients on medication for coronary heart disease (CHD) and 39 random controls matched for age group, sex, social class and locality. In vivo redox balance was the ratio of plasma antioxidant capacity, as measured with radical-trapping assay, to neutrophil respiratory burst capacity, as measured with whole blood chemiluminescence assay. Dental infections were quantitated with four rating scales. CHD patients had higher values than controls. Patients on acetosalicylic acid (ASA), diuretics or beta blockers, but not the ones on calcium channel blocker, had significantly higher redox balance than non-users. Combination of calcium channel blockers and ASA was associated with redox balance similar to taking beta blockers or diuretics. Diuretics and ASA were independent determinants of redox balance in multivariate analyses. Redox balance did not correlate with severity of dental infections (Spearman's r 0.06 to 0.11). The results contrast experimental data indicating that calcium channel blockers are as antioxidants superior to other cardiovascular drugs. Total antioxidant capacity in parallel with oxygen species production capacity should be considered in attempts to solve the antioxidant paradox.

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“…1) used clinically as racemates, although the (S)-enantiomers show stronger pharmacological activity. It is also reported that NV [23] and VER [24][25][26] have antioxidant activity against LDL. The binding study between LDL and these drugs will contribute to the elucidation of the protection mechanism of LDL autoxidation.…”

Section: Introductionmentioning

confidence: 93%

Effect of oxidation of low-density lipoprotein on drug binding affinity studied by high performance frontal analysis-capillary electrophoresis

et al. 2001

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The effect of oxidation of low-density lipoprotein (LDL) on the enantioselective drug binding affinity was investigated using high performance frontal analysis--capillary electrophoresis (HPFA-CE). Verapamil and nilvadipine enantiomers were used as the chiral model drugs. LDL was oxidized with copper sulfate for 0, 0.5, 1, 2, and 12 h at 37 degrees C. The HPFA-CE method enabled microdetermination of unbound drug concentrations in native and oxidized LDL solutions. It was found that the bindings between LDL and the model drugs were not enantioselective at any oxidation stage. The total binding affinity (nK) between LDL and verapamil enantiomers was increased by 3.3-, 4.6-, 7.0-, and 19-fold after 0.5, 1, 2, and 12 h oxidation, respectively, whereas the nK value between nilvadipine and LDLwas increased by 1.3-, 1.4-, 1.4-, and 1.7-fold in the same reaction times, respectively. These results indicate that the LDL oxidation enhances the drug binding affinity, and the affinity of verapamil is increased more sensitively than that of nilvadipine. The nK value of each model drug increased steeply after the first 2 h oxidation, followed by the gradual increase after the next 10 h oxidation. It is considered that the net increase in the negative charges and/or the formation of hydroperoxides in the first 2 h oxidation enhances the drug-LDL binding more significantly than the formation of aldehydes or Schiff bases in the following 10 h oxidation.

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A mechanism of antioxidant activity of calcium antagonist verapamil against LDL oxidation

Cited by 13 publications

References 8 publications

Can nucleotides prevent Cu-induced oxidative damage?

Can nucleotides prevent Cu-induced oxidative damage?

Neutrophil free oxygen radical production and blood total antioxidant capacity in patients with coronary heart disease using various medications^Note

Effect of oxidation of low-density lipoprotein on drug binding affinity studied by high performance frontal analysis-capillary electrophoresis

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A mechanism of antioxidant activity of calcium antagonist verapamil against LDL oxidation

Cited by 13 publications

References 8 publications

Can nucleotides prevent Cu-induced oxidative damage?

Can nucleotides prevent Cu-induced oxidative damage?

Neutrophil free oxygen radical production and blood total antioxidant capacity in patients with coronary heart disease using various medicationsNote

Effect of oxidation of low-density lipoprotein on drug binding affinity studied by high performance frontal analysis-capillary electrophoresis

Contact Info

Product

Resources

About

Neutrophil free oxygen radical production and blood total antioxidant capacity in patients with coronary heart disease using various medications^Note