A mechanism of formation of desmosome-like structures between synovial intimal cells

Ghadially, F. N.; Lalonde, J.-M. A.; Dick, C.

doi:10.1007/bf01922963

Cited by 20 publications

(7 citation statements)

References 6 publications

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“…27,28 Even though the cytoskeleton did not seem to be involved in these kind of junctions, there was an evident thickening of the cell membrane and a deposition of an unidentified extracellular material that could have had an anchoring function, allowing cells to adhere to the biomaterial fibers.…”

Section: Discussionmentioning

confidence: 96%

Chondrocyte aggregation and reorganization into three-dimensional scaffolds

Brun¹,

Abatangelo²,

Radice³

et al. 1999

J. Biomed. Mater. Res.

147

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Articular cartilage has a very limited self-repairing capacity; thus, chondral lesions normally result in chronic degeneration and, eventually, osteoarthritis development. Currently, tissue engineering offers a new tool for the clinical treatment of osteochondral defects. The present investigation aimed to develop an in vitro engineered cartilage using a new class of semisynthetic scaffolds. Two nonwoven meshes of hyaluronan esters (Hyaff(R) derivatives) were seeded with sternal chick embryo chondrocytes cultured for up to 21 days, after which time they were assessed for both the cellular growth profile and histological features. Avian chondrocytes easily adhered and proliferated onto hyaluronan-based scaffolds, demonstrating a significant preference for the fully esterified benzylic form. Histochemical staining revealed the presence of a neosynthesized glycosaminoglycan-rich extracellular matrix, and immunohistochemistry confirmed the deposition of collagen type II. Moreover, ultrastructural observations supported evidence that chondrocytes grown onto a hyaluronan-derived three-dimensional scaffold maintained their unique phenotype and organization in a cartilage-like extracellular matrix. These findings support the further pursuit of a transplantable engineered cartilage using human chondrocytes for the regeneration of chondral lesions.

show abstract

Section: Discussionmentioning

confidence: 96%

Chondrocyte aggregation and reorganization into three-dimensional scaffolds

Brun¹,

Abatangelo²,

Radice³

et al. 1999

J. Biomed. Mater. Res.

147

View full text Add to dashboard Cite

show abstract

“…The numerous desmosome-like cell-cell junctions and hemidesmosome-like junctions found at the contact sites between cell periphery and biomaterial fibers were similar to those found in native cartilage where the chondrocytes are in contact both among themselves and with the ECM. 27,28 Even though the cytoskeleton did not seem to be involved in these kind of junctions, there was an evident thickening of the cell membrane and a deposition of an unidentified extracellular material that could have had an anchoring function, allowing cells to adhere to the biomaterial fibers.…”

Section: Discussionmentioning

confidence: 99%

Chondrocyte aggregation and reorganization into three-dimensional scaffolds

Brun¹,

Abatangelo²,

Radice³

et al. 1999

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Articular cartilage has a very limited selfrepairing capacity; thus, chondral lesions normally result in chronic degeneration and, eventually, osteoarthritis development. Currently, tissue engineering offers a new tool for the clinical treatment of osteochondral defects. The present investigation aimed to develop an in vitro engineered cartilage using a new class of semisynthetic scaffolds. Two nonwoven meshes of hyaluronan esters (Hyaff derivatives) were seeded with sternal chick embryo chondrocytes cultured for up to 21 days, after which time they were assessed for both the cellular growth profile and histological features. Avian chondrocytes easily adhered and proliferated onto hyaluronan-based scaffolds, demonstrating a significant preference for the fully esterified benzylic form. Histochem-ical staining revealed the presence of a neosynthesized glycosaminoglycan-rich extracellular matrix, and immunohistochemistry confirmed the deposition of collagen type II. Moreover, ultrastructural observations supported evidence that chondrocytes grown onto a hyaluronan-derived threedimensional scaffold maintained their unique phenotype and organization in a cartilage-like extracellular matrix. These findings support the further pursuit of a transplantable engineered cartilage using human chondrocytes for the regeneration of chondral lesions.

show abstract

“…[70,72,731; our unpublished results). It also suggests that the "desmosome-like" junctions described in diseased synovial tissues [70,72,73,791, including "synoviomas" (e.g. [95]), are not true desmosomes but probably special forms of "intermediate junctions ".…”

Section: Human Tissuesmentioning

confidence: 99%