A mechanistic investigation about hepatoxic effects of borneol using zebrafish

Abstract

Except for clinical value, borneol is routinely used in food and cosmetics with seldom safety evaluation. To investigate its hepatoxicity, we exposed 3 dpf (days post fertilization) larval zebrafish to borneol at a gradient of concentrations (200–500 μM) for 3 days. Herein, our results revealed that high doses of borneol (300–500 μM) caused liver size decrease or lateral lobe absence. Borneol also seriously disturbed the hepatic protein metabolism presented with the increased activity of alanine aminotransferase (ALT) and lipid metabolism shown with the increased level of triglycerides (TG) and total cholesterol (TC). The lipid accumulation (oil red staining) was detected as well. Additionally, significant upregulation of genes was detected that related to oxidative stress, lipid anabolism, endoplasmic reticulum stress (ERS), and autophagy. Conversely, the lipid metabolism-related genes were markedly downregulated. Moreover, the changes in the superoxide dismutase activity and the level of glutathione and malondialdehyde raised the likelihood of lipid peroxidation. The outcomes indicated the involvement of oxidative stress, ERS, lipid metabolism, and autophagy in borneol-induced lipid metabolic disorder and hepatic injury. This study will provide a more comprehensive understanding of borneol hepatoxicity and the theoretical basis for the safe use of this compound.

The enhanced hepatotoxicity of isobavachalcone in depigmented zebrafish due to calcium signaling dysregulation and lipid metabolism disorder

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Proteomics Reveals the Role of Paederia scandens in ameliorating Non-alcoholic Fatty Liver Disease in a Rat Model

“…Profiles of 14 differentially abundant keystone proteins (up, upregulated; down, downregulated)(Altınok-Yipel et al 2019;Mazani et al 2022;Ola & Sofolahan 2021). Moreover, accumulating evidence indicates that borneol, one of the main ingredients of Paederia scandens, is responsible for mitigating inflammation in the liver(Liu et al 2023;Madhuri & Naik …”