A Mechanistic Link between<i>STM</i>and<i>CUC1</i>during Arabidopsis Development

Spinelli, Silvana Virginia; Martín, A.; Viola, Ivana L.; González, Daniel H.; Palatnik, Javier F.

doi:10.1104/pp.111.177709

Cited by 123 publications

(111 citation statements)

References 58 publications

Supporting

Mentioning

106

Contrasting

Unclassified

Order By: Relevance

“…The other class I KNOX genes are expressed and perform redundant, albeit weaker, functions compared with STM in the SAM, with BP/ KNAT1 and KNAT6 playing remarkable roles during flower gynoecium development (Arnaud and Pautot, 2014). Because the induction of class I KNOX genes can cause the up-regulation of CUC genes (Spinelli et al, 2011), future investigation is necessary to determine whether the elevated expression of CUC1, CUC2, and CUC3 is a secondary effect in the atzrf1a atzrf1b mutant. Within the SAM, WUS is expressed specifically in the stem cell organizing center and acts both independently and complementarily with STM in the maintenance of stem cell activity (Laux et al, 1996;Mayer et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

ZRF1 Chromatin Regulators Have Polycomb Silencing and Independent Roles in Development

Feng

Chen²,

Berr³

et al. 2016

Plant Physiol.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

ZRF1 Chromatin Regulators Have Polycomb Silencing and Independent Roles in Development

Feng

Chen²,

Berr³

et al. 2016

Plant Physiol.

View full text Add to dashboard Cite

“…19,49 Although functionally connected, the expression of CUC1, CUC2 and CUC3 genes is regulated through different pathways. This is also reflected by their expression profiles, which are not identical in the SAM, 25,34,45 Fig. 1B In addition to transcriptional control, CUC1 and CUC2 are subjected to post-transcriptional regulation through the micro-RNA pathway.…”

mentioning

confidence: 99%

“…They play an essential role in shoot meristem initiation through the regulation of STM expression. 1,9,19,29,34,45,49 Recently it has been reported that CUC1 and CUC2 are also required for formation and stable positioning of the carpel margin meristems. 23 Depletion of these genes leads to defects in cotyledon separation, organ fusions and cup-shaped cotyledons.…”

mentioning

confidence: 99%

Interplay between miRNA regulation and mechanical stress forCUCgene expression at the shoot apical meristem

Fal

Landrein

Hamant

2016

Plant Signaling & Behavior

View full text Add to dashboard Cite

The shoot apical meristem is the central organizer of plant aerial organogenesis. The molecular bases of its functions involve several cross-talks between transcription factors, hormones and microRNAs. We recently showed that the expression of the homeobox transcription factor STM is induced by mechanical perturbations, adding another layer of complexity to this regulation. Here we provide additional evidence that mechanical perturbations impact the promoter activity of CUC3, an important regulator of boundary formation at the shoot meristem. Interestingly, we did not detect such an effect for CUC1. This suggests that the robustness of expression patterns and developmental programs is controlled via a combined action of molecular factors as well as mechanical cues in the shoot apical meristem. KEYWORDSCUC genes; mechanical stress; Meristem; micro-RNAs Throughout their lifetime, plants are exposed to a number of external mechanical perturbations, such as wind or touch. This leads to changes in mRNA level of many genes, and long-term developmental responses such as stem thickening and flowering delay 5 In addition to these external factors, plants are also constantly affected by intrinsic tensile stresses, notably because plant cells are under high turgor pressure. There is now accumulating evidence that these internal stresses are affecting many aspects of the cells, thus channeling growth in the long term.14 The shoot apical meristem (SAM) is the central organizer of plant aerial organogenesis. Initiation of new organs and maintenance of SAM is achieved through the concomitant action of multiple regulatory pathways. Genetic screens have identified many key players, including transcription factors, hormones, and microRNAs. 38,48,53,55 In addition to these biochemical factors, mechanical forces are also present within the meristem. In particular, the boundary domain that separates newly emerging organ from the meristem is under highly anisotropic tensile stresses. 4,15,32 A key question for the future is the analysis of the interplay between mechanical forces and the molecular regulators of meristem function (Fig. 1A).In a recent article, 26 we showed that a master regulator of meristem maintenance, the homeodomain protein SHOOT MERISTEMLESS (STM) is expressed at a higher level in the boundary domain, and that this local increase in promoter activity can be related to mechanical stress: mechanical perturbations are sufficient to induce STM expression in the meristem. Interestingly, mechanical perturbations do not affect all boundary-expressed genes in the same way. For instance, the promoter activity of the PINOID gene, which is also increased in the boundary domain, is not affected by mechanical perturbations. 26Here we focus on the most canonical genetic markers of boundary identity, the CUP SHAPED COTYLEDON (CUC) genes. CUC1, CUC2 and CUC3, belong to a group of NAC domain transcription factors and show a high level of functional redundancy. They play an essential role in shoot meristem initiation throug...

show abstract

“…A recent report shows that SHOOT MERISTEMLESS (STM) acts as a direct activator of CUC1 through CTGTCA elements found in the CUC1 promoter (Spinelli et al, 2011). Prolonged ectopic expression of STM also leads to activation of CUC2 and MIR164A, but this effect is indirect (Spinelli et al, 2011).…”

Section: Pcg-mediated Repression At Several Levels Of Leaf Margin Devmentioning

confidence: 99%

DEVELOPMENT-RELATED PcG TARGET IN THE APEX 4 controls leaf margin architecture in Arabidopsis thaliana

et al. 2012

View full text Add to dashboard Cite

In a reverse genetics screen based on a group of genes enriched for development-related Polycomb group targets in the apex (DPAs), we isolated DPA4 as a novel regulator of leaf margin shape. T-DNA insertion lines in the DPA4 locus display enhanced leaf margin serrations and enlarged petals, whereas overexpression of DPA4 results in smooth margins. DPA4 encodes a putative RAV (Related to ABI3/VP1) transcriptional repressor and is expressed in the lateral organ boundary region and in the sinus of leaf serrations. DPA4 expression domains overlap with those of the known leaf shape regulator CUP-SHAPED COTYLEDON 2 (CUC2) and we provide evidence that DPA4 negatively regulates CUC2 expression independently of MIR164A, an established regulator of CUC2. Taken together, the data suggest DPA4 as a newly identified player in the signalling network that controls leaf serrations in Arabidopsis thaliana.

show abstract

A Mechanistic Link betweenSTMandCUC1during Arabidopsis Development

Cited by 123 publications

References 58 publications

ZRF1 Chromatin Regulators Have Polycomb Silencing and Independent Roles in Development

ZRF1 Chromatin Regulators Have Polycomb Silencing and Independent Roles in Development

Interplay between miRNA regulation and mechanical stress forCUCgene expression at the shoot apical meristem

DEVELOPMENT-RELATED PcG TARGET IN THE APEX 4 controls leaf margin architecture in Arabidopsis thaliana

Contact Info

Product

Resources

About