A Mediated BOD Biosensor Based on Immobilized B. Subtilis on Three-Dimensional Porous Graphene-Polypyrrole Composite

Hu, Jingfang; Li, Yueqi; Gao, Guowei; Xia, Shanhong

doi:10.3390/s17112594

Cited by 42 publications

(19 citation statements)

References 36 publications

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“…In general, the peaks at around 21° were attributed to the polar van der Waals force between pyrrole groups and interaction scattering from pyrrole-counterion . Moreover, a stronger peak at around 2θ = 25° was attributed to GO crystalline structure in the pattern of prepared GO/PPy composite. , The addition of GO or CFGO into PPy sheath induced the slight right deviation of the peak at 2θ = 21.4 or 22.2°, confirming the existence of stronger van der Waals’ force interaction between PPy and GO (or CFGO) sheets …”

Section: Results and Discussionmentioning

confidence: 70%

Preparation of Graphene Oxide-Doped Polypyrrole Composite Films with Stable Conductivity and Their Effect on the Elongation and Alignment of Neurite

Shang

Huang

et al. 2019

ACS Biomater. Sci. Eng.

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Conductive composite films (CCFs) of polypyrrole (PPy) doped with sodium dodecyl benzenesulfonate (DBS) and graphene oxide (GO) were prepared via electrochemical deposition of PPy nanoparticles on the aligned PLLA fibers. The doping amount of GO in CCF sheaths reached to ∼3.8%, leading to ∼32 S cm–1 of the mean conductivity of DBS-GO-doped CCF. The electrical potential image of DBS-GO-doped CCFs showed that the charges were mainly distributed on the tops of GO sheets protruded from the PPy surface. The immersion results indicated that the more stable conductivity of DBS-GO-doped CCF resulted from the reservation of GO sheets in the PPy film because of the van der Waals’s force between GO and the pyrrole rings in CCF sheath. The results of PC12 cell test showed that, the rougher surface of DBS-GO-doped CCF could positively affect the neurite elongation due to the slight protrusion of GO sheets from the CCF surface. The electrical stimulation (ES) of 50 mV cm–1 through DBS-GO-doped CCF could significantly promote neurite elongation and alignment, as well ∼149 μm of the mean neurite length and 70% of alignment on DBS-GO-doped CCF were significantly higher than those without ES, respectively. These results indicated that the more stable conductivity of DBS-GO-doped CCF could enhance the ES efficiency on the elongation and alignment of neurites, due to the more charges accumulation on the tops of the protruded GO sheets in the CCF surface. A mechanism was proposed to explain the positive effect of the GO-nanosheets protrusion, rougher PPy surface and stable conductivity on the elongation and alignment of neurites. These results suggested the possibilities for the long-term peripheral nerve repair in vivo via ES through DBS-GO-doped CCF.

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Section: Results and Discussionmentioning

confidence: 70%

Preparation of Graphene Oxide-Doped Polypyrrole Composite Films with Stable Conductivity and Their Effect on the Elongation and Alignment of Neurite

Shang

Huang

et al. 2019

ACS Biomater. Sci. Eng.

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“…In this sense, Hu and collaborators [85] modified graphene oxide prior to the electrochemical step (Figure 8). A rGO-Polypyrrole set was obtained by adding 500 µL of pyrrole monomer to 10 mL of GO solution.…”

Section: Multiple-step Strategymentioning

confidence: 99%

Nanocomposite Materials Based on Electrochemically Synthesized Graphene Polymers: Molecular Architecture Strategies for Sensor Applications

et al. 2021

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The use of graphene and its derivatives in the development of electrochemical sensors has been growing in recent decades. Part of this success is due to the excellent characteristics of such materials, such as good electrical and mechanical properties and a large specific surface area. The formation of composites and nanocomposites with these two materials leads to better sensing performance compared to pure graphene and conductive polymers. The increased large specific surface area of the nanocomposites and the synergistic effect between graphene and conducting polymers is responsible for this interesting result. The most widely used methodologies for the synthesis of these materials are still based on chemical routes. However, electrochemical routes have emerged and are gaining space, affording advantages such as low cost and the promising possibility of modulation of the structural characteristics of composites. As a result, application in sensor devices can lead to increased sensitivity and decreased analysis cost. Thus, this review presents the main aspects for the construction of nanomaterials based on graphene oxide and conducting polymers, as well as the recent efforts made to apply this methodology in the development of sensors and biosensors.

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“…The method technically realizes the rapid determination of BOD (measurement time varies from several minutes to several hours), but it has some deficiencies in practical application, mainly including low efficiency and high selectivity due to single species of immobilized microorganisms, and complex immobilization method. Thus, considerable effort has been devoted to developing biofilm constructed with mixed microorganisms [5,6] or activated sludge [7], and various immobilization methods [8][9][10] to circumvent the limitations. It has made great progress in these studies and many optimized BOD biosensors have been structured.…”

Section: Introductionmentioning

confidence: 99%

Integrated Determination of Chemical Oxygen Demand and Biochemical Oxygen Demand

Qi¹,

Han²,

Zhao³

et al. 2021

Pol. J. Environ. Stud.

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Chemical oxygen demand (COD) and biochemical oxygen demand (BOD) with irreplaceable significance are comprehensive indicators for evaluating water quality. Effective control of water pollution depends on the rapid determination of these indicators. In the study, the rapid determination of BOD was carried out by activated sludge aeration degradation method. The BOD quantification was achieved by measuring the COD change before and after microbial degradation (BOD ΔCOD). Singlefactor experiment was employed to investigate the effect of operating parameters on the determination of BOD. In addition, the interactions of main variables were evaluated by Box-Behnken Design, and the results showed that the interaction term of reaction time and initial pH value had a significant effect. The optimum conditions for determination of BOD were reaction time of 273.68 min, dosage of activated sludge of 8.72 mL, and pH of 7.18. Under the optimal conditions, the correlation experiment showed that BOD ΔCOD and BOD 5 exhibited a good linear relationship in the concentration range of 0-500 mg/L, and the correlation coefficient R 2 was 0.996. It indicated that the method realized not only the rapid determination of BOD, but also the integrated determination of COD and BOD.

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A Mediated BOD Biosensor Based on Immobilized B. Subtilis on Three-Dimensional Porous Graphene-Polypyrrole Composite

Cited by 42 publications

References 36 publications

Preparation of Graphene Oxide-Doped Polypyrrole Composite Films with Stable Conductivity and Their Effect on the Elongation and Alignment of Neurite

Preparation of Graphene Oxide-Doped Polypyrrole Composite Films with Stable Conductivity and Their Effect on the Elongation and Alignment of Neurite

Nanocomposite Materials Based on Electrochemically Synthesized Graphene Polymers: Molecular Architecture Strategies for Sensor Applications

Integrated Determination of Chemical Oxygen Demand and Biochemical Oxygen Demand

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