A mediated polyphenol oxidase biosensor immobilized by electropolymerization of 1,2-diamino benzene

Akyılmaz, Erol; Kozgus, Ozge; Türkmen, Hayati; Çetınkaya, Bekır

doi:10.1016/j.bioelechem.2009.09.003

Cited by 24 publications

(10 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For that reason, it was found that neutral PB is the most convenient for following experiments. It was experimentally confirmed that the highest current response of Trolox at the developed CPE/CNTs/TYR/Nafion ® biosensor was obtained at pH 7.0, which coincides with the previously obtained results [15,23].…”

Section: Selection Of Optimal Ph Value Of Working Mediumsupporting

confidence: 90%

Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes

et al. 2020

View full text Add to dashboard Cite

In this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion® layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied with respect to the sensitive amperometric detection of total content of phenolic compounds in berries, expressed as concentration equivalent of Trolox. After optimization of key instrumental and electroanalytical parameters, the biosensor was used for determination of TEAC in blackberries, blueberries, cranberries, raspberries and strawberries by method of multiple standard additions. Electrochemical TEAC assays corresponded well with results obtained by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl radical method, known as DPPH assay. Obtained values were compared with those listed in the National Nutrient Database for additional antioxidant capacity assays as well.

show abstract

Section: Selection Of Optimal Ph Value Of Working Mediumsupporting

confidence: 90%

Determination of Trolox Equivalent Antioxidant Capacity in Berries Using Amperometric Tyrosinase Biosensor Based on Multi-Walled Carbon Nanotubes

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Redox mediators have a fast electrontransfer rate between the active site of the enzyme and electrode surface [34]. These mediators could be bound to the redox proteins (oxidoreductases) by histidine residues [34]. Mediator modified enzyme showed improved electron-transfer rates and rates of catalytic reactions compared with native protein because the center is closely located to the protein globule surface (less than 1 nm).…”

Section: Enzyme Biosensor Characteristicmentioning

confidence: 98%

“…HBTC) have been described and combined with a variety of different redox enzymes in biosensors. Redox mediators have a fast electrontransfer rate between the active site of the enzyme and electrode surface [34]. These mediators could be bound to the redox proteins (oxidoreductases) by histidine residues [34].…”

Section: Enzyme Biosensor Characteristicmentioning

confidence: 99%

Active Langmuir–Schaefer films of tyrosinase—Characteristic

Cabaj

Sołoducho

Świst

2010

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

“…24 Other research reported a biosensor for the detection of 1,2-diaminobenzene (DAB) by electropolymerization of PPO, DAB, and a mediator complex on a screen-printed Pt electrode. 25 The analytical results for DAB demonstrated that the mediator-modified enzymes showed improved electron-transfer rates compared to that of the native enzyme. Recently, other efficient immobilization techniques have been developed.…”

Section: Electrochemical Detection Of Polyphenolsmentioning

confidence: 98%