A mediatorless and label-free amperometric immunosensor for detection of h-IgG

Zhang, Lingyan; Liu, Yan; Chen, Tao

doi:10.1016/j.ijbiomac.2008.04.010

Cited by 28 publications

(5 citation statements)

References 29 publications

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“…The sensor also offers low limit of detection of 0.15 ng mL À1 , limit of quantitation of 0.50 ng mL À1 and high sensitivity (11.297 lA cm À2 ), which are sufficient for the detection of immunoglobulin G at the physiological level. Table 1 shows the detection performances for detecting immunoglobulin G using our proposed immunosensor as compared to other immunosensors reported in the literatures [32][33][34][35][36][37][38][39]. Entries 1-4 are of sandwich structured immunosensors whilst entries 5-8 are of label-free immunosensors.…”

Section: Linear Response and Limit Detection Of The Immunosensormentioning

confidence: 99%

A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G

Putnin

Jumpathong

Laocharoensuk

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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This work focuses on fabricating poly(2-aminobenzylamine)-modified screen-printed carbon electrode as an electrochemical immunosensor for the label-free detection of human immunoglobulin G. To selectively detect immunoglobulin G, the anti-immunoglobulin G antibody with high affinity to immunoglobulin G was covalently linked with the amine group of poly(2-aminobenzylamine) film-deposited screen-printed carbon electrode. The selectivity for immunoglobulin G was subsequently assured by being challenged with redox-active interferences and adventitious adsorption did not significantly interfere the analyte signal. To obviate the use of costly secondary antibody, the [Fe(CN)] redox probe was instead applied to measure the number of human immunoglobulin G through the immunocomplex formation that is quantitatively related to the level of the differential pulse voltammetric current. The resulting immunosensor exhibited good sensitivity with the detection limit of 0.15 ng mL, limit of quantitation of 0.50 ng mL and the linear range from 1.0 to 50 ng mL. Given those striking analytical performances and the affordability arising from using cheap screen-printed carbon electrode with label-free detection, the immunosensor serves as a promising model for the next-step development of a diagnostic tool.

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Section: Linear Response and Limit Detection Of The Immunosensormentioning

confidence: 99%

A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G

Putnin

Jumpathong

Laocharoensuk

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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show abstract

“…This immunosensors are based in the changes of the electrical properties by recognition of antigen/antibody layer immobilized on electrodes. The major drawback of impedance methods to biosensors is the need of an interfacial engineering to reduce non-specific adsorptions (166)(167)(168).…”

Section: Impedimetric Immunosensor Applied For Diseasesmentioning

confidence: 99%

Recent advances in nano-based electrochemical biosensors application in diagnosis and monitoring of diseases

Wc²,

Jm³

et al. 2011

Front Biosci

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Based on biological molecules combined with nanostructured components, the news generations of electrochemical biosensors can employ different transducers (potentiometric, amperometric and impedimetric) converting the chemical information into a measurable amperometric signal. Following this contemporary theme, our main focus in this review is to discuss different methodologies for application in biosensing, whose signal transduction is based on electrochemical principles. We apply a discussion on recent trends involving different nanostructured materials, but without daring to contemplate all nanomaterials incessantly cited in literature, which leads us to believe that this moment is an unprecedented revolution in the preparation of electrochemical biodevices. Besides, some structures of bio-nano interface and different electrochemical biosensors involved in diagnosis systems are also discussed. We outline in several parts of the report how nanoscience technologies are emerging in diagnostic medicine, as well as convergence of electrochemistry and bio-nanoscience. Our hopes for this review are that it can help different categories of researchers to understand the broad application area of electrochemistry and bioelectrochemistry, in order to detecting several types of diseases and biological phenomena.

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“…In recent years, the electrochemical immunosensors have been employed in detecting the many kinds of biomarker proteins for the disease and virus-infection diagnoses (Rama and Costa-García, 2016 ; Norfun et al, 2017 ). With some exceptional advantages such as cost-effective instrumentation, less complexity in operation, and fast detection, label-free immunosensors have attracted research development in the diagnoses (Zhang et al, 2008 , 2017 ; Qiu et al, 2010 ; Jumpathong et al, 2016 ; Li et al, 2016 ; Tabrizi et al, 2016 ; Wang and Ma, 2018 ; Chanarsa et al, 2020 ; Kuntamung et al, 2021 ). The immunosensors are also constructed with no complex fabrication process, leading to convenience of use (Norfun et al, 2016 , 2017 ; Putnin et al, 2018 ; Wang and Ma, 2018 ; Zhao et al, 2018 ; Kuntamung et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

A Redox Cu(II)-Graphene Oxide Modified Screen Printed Carbon Electrode as a Cost-Effective and Versatile Sensing Platform for Electrochemical Label-Free Immunosensor and Non-enzymatic Glucose Sensor

et al. 2021

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A novel copper (II) ions [Cu(II)]-graphene oxide (GO) nanocomplex-modified screen-printed carbon electrode (SPCE) is successfully developed as a versatile electrochemical platform for construction of sensors without an additionally external redox probe. A simple strategy to prepare the redox GO-modified SPCE is described. Such redox GO based on adsorbed Cu(II) is prepared by incubation of GO-modified SPCE in the Cu(II) solution. This work demonstrates the fabrications of two kinds of electrochemical sensors, i.e., a new label-free electrochemical immunosensor and non-enzymatic sensor for detections of immunoglobulin G (IgG) and glucose, respectively. Our immunosensor based on square-wave voltammetry (SWV) of the redox GO-modified electrode shows the linearity in a dynamic range of 1.0–500 pg.mL−1 with a limit of detection (LOD) of 0.20 pg.mL−1 for the detection of IgG while non-enzymatic sensor reveals two dynamic ranges of 0.10–1.00 mM (sensitivity = 36.31 μA.mM−1.cm−2) and 1.00–12.50 mM (sensitivity = 3.85 μA.mM−1.cm−2) with a LOD value of 0.12 mM. The novel redox Cu(II)-GO composite electrode is a promising candidate for clinical research and diagnosis.

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A mediatorless and label-free amperometric immunosensor for detection of h-IgG

Cited by 28 publications

References 29 publications

A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G

A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G

Recent advances in nano-based electrochemical biosensors application in diagnosis and monitoring of diseases

A Redox Cu(II)-Graphene Oxide Modified Screen Printed Carbon Electrode as a Cost-Effective and Versatile Sensing Platform for Electrochemical Label-Free Immunosensor and Non-enzymatic Glucose Sensor

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