A megakaryocyte analysis of the bone marrow in patients with myelodysplastic syndrome, myeloproliferative disorder and allied disorders

Abstract

A morphometric analysis was performed on aspirate clots of bone marrow to identify the presence of atypical megakaryocytes after immunohistological staining with a monoclonal antibody against Factor VIII. This study included cases of myelodysplastic syndrome (MDS), myeloproliferative disorder (MPD), aplastic anaemia (AA), idiopathic thrombocytopenic purpura (ITP), chronic myelogenous leukaemia (CML), and control cases free from any haematological disease. Quantitative and qualitative abnormalities of megakaryocytes were assessed using an image analyser and a personal computer to perform a morphometric analysis of the number (per mm2), arrangement (microns), nuclear size (microns2), cell size (microns2), nuclear size/cell size (N/C) ratio, and nuclear contour index (NCI). Micromononuclear megakaryocytes were detected in MDS, while in MPD, large over-mature magakaryocytes were observed to increase in number. In AA, the megakaryocytes decreased dramatically in number without showing any morphological abnormality. In CML, the megakaryocytes also increased without any remarkable morphological abnormality. In ITP, only the NCI increased. The above results show that MDS is characterized by the presence of atypical micromegakaryocytes, while MPD is characterized by atypical large over-mature megakaryocytes.

Classical Swine Fever: Pathology of Bone Marrow

“…Such changes include an increase in microMK numbers, the predominance of MKs with cloud-shaped nuclei, and a decrease in both cytoplasmic area and the ratio of nuclear area to total area. These changes are characteristic of the state of dysmegakaryocytopoiesis reported in myelodysplastic syndromes 19 and in HIV infection. 2,28 Proliferation of reticulin fibers is also a common finding in this type of pathology.…”

“…2,28 Proliferation of reticulin fibers is also a common finding in this type of pathology. 19,30 Impaired megakaryocytopoiesis in CSF may be related to various events taking place during the course of the disease. Chief among these are 1) the infection of stromal cells, mainly macrophages and reticular cells, both of which play a major role in regulating hematopoiesis in general and megakaryocytopoiesis in particular, through soluble chemical factors 1,13,32,33 and 2) depletion of T lymphocytes, which are also involved in modulating hematopoiesis.…”

Megakaryocyte Size and Concentration in the Bone Marrow of Thrombocytopenic and Nonthrombocytopenic Neonates

“…However, these features are not present in all cases. Patients with chronic ITP, in particular, often do not have increased megakaryocyte concentrations (27,28) and exhibit decreased concentrations of megakaryocyte colonyforming units (CFU-MK) in megakaryocyte progenitor assays (29). Many of these patients have been shown to have antiplatelet antibodies that also react with megakaryocytes in the bone marrow and can inhibit their proliferation (30).…”

Clonality investigation of morphologically dysplastic hematopoietic cells in myelodysplastic syndrome marrows

“…Some authors [14,19] ever reported decreased ploidy (population was the largest in the 8N, not as the 16N in normal controls) in ordinary morphological recognized megakaryocytes on MDS marrow smears by measuring nuclear DNA content. But for micro-megakaryocytes (defined by CD61 sorting and CD41 restaining, which usually be ignored under ordinary staining), the 2N ploidy were in the absolutely ascendant, which represented the morphological immature and functional dysplasia of MDS megakaryocytes responsible for clinical dependence on platelet transfusion for reduced ploidy of megakaryocytes are associated with decreased rate of platelet production [26].…”