A Membrane-Anchored Protein Kinase Involved in
            <i>Brassica</i>
            Self-Incompatibility Signaling

Murase, Kohji; Shiba, Hiroshi; Iwano, Megumi; Che, Fang‐Sik; Watanabe, Masao; Isogai, Akira; Takayama, Seiji

doi:10.1126/science.1093586

Cited by 226 publications

(239 citation statements)

References 24 publications

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“…Although receptor-like cytoplasmic kinases (RLCKs) account for at least 125 of the 610 annotated RLKs in Arabidopsis (Arabidopsis thaliana), much remains to be learned about their functions within cell signaling complexes (Shiu and Bleecker, 2001;Goring and Walker, 2004;Shiu et al, 2004;Jurca et al, 2008). Several of the 46 RLCKs assigned to the class VII subfamily have been found to function in pathogen response and developmental signaling pathways (Swiderski and Innes, 2001;Shao et al, 2003;Murase et al, 2004;Muto et al, 2004;Veronese et al, 2006;Ade et al, 2007;Lu et al, 2010;Zhang et al, 2010;Liu et al, 2011).…”

mentioning

confidence: 99%

“…The M-Locus Protein Kinase (MLPK) RLCK functions as a coreceptor of a ligand-binding RLK to transduce signaling. During the self-incompatibility response in Brassica rapa flowers, MLPK has been found to interact with the ligandactivated S-locus receptor kinase and is essential for the cell signaling leading to rejection of self pollen (Murase et al, 2004;Kakita et al, 2007). The BOTRYTIS-INDUCED KINASE1 (BIK1) RLCK functions in a signaling relay with an activated ligand-binding RLK and its coreceptor.…”

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confidence: 99%

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CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

et al. 2011

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Receptor-like kinase-mediated cell signaling pathways play fundamental roles in many aspects of plant growth and development. A pair of Arabidopsis (Arabidopsis thaliana) leucine-rich repeat receptor-like kinases (LRR-RLKs), HAESA (HAE) and HAESA-LIKE2 (HSL2), have been shown to activate the cell separation process that leads to organ abscission. Another pair of LRR-RLKs, EVERSHED (EVR) and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1, act as inhibitors of abscission, potentially by modulating HAE/HSL2 activity. Cycling of these RLKs to and from the cell surface may be regulated by NEVERSHED (NEV), a membrane trafficking regulator that is essential for organ abscission. We report here the characterization of CAST AWAY (CST), a receptor-like cytoplasmic kinase that acts as a spatial inhibitor of cell separation. Disruption of CST suppresses the abscission defects of nev mutant flowers and restores the discrete identity of the trans-Golgi network in nev abscission zones. After organ shedding, enlarged abscission zones with obscured boundaries are found in nev cst flowers. We show that CST is a dual-specificity kinase in vitro and that myristoylation at its amino terminus promotes association with the plasma membrane. Using the bimolecular fluorescence complementation assay, we have detected interactions of CST with HAE and EVR at the plasma membrane of Arabidopsis protoplasts and hypothesize that CST negatively regulates cell separation signaling directly and indirectly. A model integrating the potential roles of receptor-like kinase signaling and membrane trafficking during organ separation is presented.

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confidence: 99%

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CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

et al. 2011

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“…SRK exists as a homodimer and is proposed to interact as a transient complex with the M locus protein kinase (MLPK ) (Murase et al 2004 ;Kakita et al 2007a , b ). B. rapa MLPK is a receptor-like cytoplasmic kinase that is also localized to the plasma membrane in the stigma (Murase et al 2004 ). MLPK exists as two splice variants with different N-terminal ends.…”

Section: Self-incompatibility In the Genus Arabidopsismentioning

confidence: 99%

Signaling Events in Pollen Acceptance or Rejection in the Arabidopsis Species

Indriolo

Safavian

Goring

2014

Sexual Reproduction in Animals and Plants

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The initial events of pollen-pistil interactions are fundamentally important in fl owering plants because they infl uence successful fertilization. These early events include the recognition of pollen grains through signaling events in the pistil that will lead to the acceptance of a compatible pollen grain or the rejection of an incompatible pollen grain. There has been much research into this fi eld in the Brassicaceae, as this family includes many agriculturally important crops such as canola, radish, turnip, and cabbage. However, this review focuses on what is known about the early pollen-pistil interactions in the experimentally tractable Arabidopsis genus, including Arabidopsis thaliana (a self-compatible species) and Arabidopsis lyrata (a self-incompatible species). Compatible pollinations are driven by the ability of the pistil to provide the resources for an acceptable pollen grain to hydrate, germinate, and fertilize the ovule. Self-incompatible species have a receptorligand signaling pathway that rejects self-pollen grains, preventing inbreeding and encouraging genetic diversity within the species. There is some overlap between these two pathways, and current research is looking for unknown elements and downstream events following the initial recognition of a pollen grain in Arabidopsis .

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“…To date, the only candidates for signaling molecules acting downstream of SP11/ SRK have been MLPK, the membrane-anchored M -locus protein kinase (Murase et al 2004 ), and ARC1, an arm repeat-containing protein with E3 ubiquitin-ligase (Stone et al 1999 ) (Fig. 21.3 ).…”

Section: Si Signaling Cascade Leading To Rejection Of Self-pollenmentioning

confidence: 99%

“…MLPK was identifi ed as a positive mediator of SI signaling in a genetic analysis of a self-compatible mutant of B. rapa var. Yellow Sarson (Murase et al 2004 ). Upon self-pollination, MLPK is thought to interact directly with SRK to form an SRK-MLPK receptor complex on the plasma membrane and enhance SI signaling (Kakita et al 2007 ).…”

Section: Si Signaling Cascade Leading To Rejection Of Self-pollenmentioning

confidence: 99%

Self-Incompatibility in the Brassicaceae

Iwano

Itô

Shimosato-Asano

et al. 2014

Sexual Reproduction in Animals and Plants

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Self-incompatibility (SI) in angiosperms prevents inbreeding and promotes outcrossing to generate genetic diversity. SI in the Brassicaceae is controlled by the S -haplotype-specifi c interaction between pollen ligand ( S -locus protein 11, SP11 or SCR) and its stigmatic receptor ( S -receptor kinase, SRK ). SP11/ SCR binding to cognate SRK induces autophosphorylation of SRK, which triggers a signaling cascade leading to the rejection of self-pollen. However, the mechanism of self-pollen rejection downstream of this ligand-receptor interaction is unknown. Here, we generated self-incompatible Arabidopsis thaliana accession C24 for the forward-genetic approach and live-cell imaging of SI in the Brassicaceae. Furthermore, for reverse-genetic analysis, we extended the Arabidopsis Targeting Induced Local Lesions IN Genomes (TILLING) resources by developing a new population of ethyl methanesulfonate (EMS)-induced mutant lines in A. thaliana accession C24. We believe that the reverse-genetic approach is a useful tool for identifying genes that function in the SI signaling pathway of the Brassicaceae.

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A Membrane-Anchored Protein Kinase Involved in Brassica Self-Incompatibility Signaling

Cited by 226 publications

References 24 publications

CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

CAST AWAY, a Membrane-Associated Receptor-Like Kinase, Inhibits Organ Abscission in Arabidopsis

Signaling Events in Pollen Acceptance or Rejection in the Arabidopsis Species

Self-Incompatibility in the Brassicaceae

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